Magnesium in PDB 5ez6: Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa

The structure of Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa, PDB code: 5ez6 was solved by Z.Yan, S.Ma, Y.Zhang, L.Ma, F.Wang, J.Li, L.Miao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.70 / 1.80
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	91.407, 91.407, 56.202, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / 21.3

The binding sites of Magnesium atom in the Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa (pdb code 5ez6). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa, PDB code: 5ez6:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:29.2 occ:1.00 O B:VAL38 2.8 16.6 1.0 N B:GLU40 3.0 15.5 1.0 CA B:PHE39 3.2 15.2 1.0 C B:VAL38 3.4 17.6 1.0 CB B:TYR42 3.5 16.1 1.0 C B:PHE39 3.6 15.5 1.0 N B:PHE39 3.6 15.3 1.0 O B:GLU40 3.8 14.4 1.0 CD2 B:TYR42 3.8 17.8 1.0 CA B:TYR42 4.1 15.5 1.0 CA B:GLU40 4.1 16.6 1.0 CG B:TYR42 4.2 16.4 1.0 C B:GLU40 4.2 16.4 1.0 N B:TYR42 4.3 14.9 1.0 CB B:PHE39 4.4 16.1 1.0 CG2 B:VAL38 4.5 22.2 1.0 CG B:PRO36 4.6 22.1 1.0 CB B:GLU40 4.7 18.6 1.0 O B:PHE39 4.8 15.5 1.0 CA B:VAL38 4.8 19.4 1.0 CG B:PHE39 4.9 16.4 1.0 CD2 B:PHE39 4.9 16.2 1.0

Magnesium binding site 2 out of 3 in the Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:30.5 occ:1.00 O B:CYS83 2.8 15.8 1.0 O B:SER91 2.9 18.2 1.0 N B:ASP13 3.0 15.4 1.0 CG B:ASP13 3.5 18.9 1.0 OD1 B:ASP13 3.6 16.4 1.0 NE1 B:TRP99 3.6 14.7 1.0 CB B:ASP13 3.6 18.1 1.0 CA B:GLY12 3.7 14.8 1.0 C B:SER91 3.7 18.9 1.0 CD1 B:TRP99 3.7 13.9 1.0 CA B:PHE84 3.8 17.8 1.0 C B:GLY12 3.8 14.8 1.0 C B:CYS83 3.8 16.7 1.0 CB B:SER91 3.8 19.2 1.0 OD2 B:ASP13 3.9 17.2 1.0 CB B:PHE84 3.9 18.6 1.0 CA B:ASP13 3.9 16.0 1.0 CA B:SER91 4.0 19.1 1.0 CD1 B:ILE95 4.0 17.6 1.0 N B:PHE84 4.2 16.0 1.0 CG1 B:ILE95 4.5 17.5 1.0 OG B:SER91 4.6 17.9 1.0 CB B:ASN94 4.6 18.5 1.0 SG B:CYS16 4.6 22.3 1.0 CE2 B:TRP99 4.8 14.5 1.0 N B:LEU92 4.9 18.8 1.0 CG B:TRP99 4.9 13.5 1.0 N B:ILE95 5.0 17.7 1.0

Magnesium binding site 3 out of 3 in the Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg203 b:32.4 occ:1.00 O B:HOH359 2.7 17.4 1.0 O B:GLY17 2.8 14.2 1.0 N7 B:GDP204 2.8 22.3 1.0 CB B:ALA161 3.4 19.4 1.0 CA B:GLY17 3.4 16.3 1.0 C B:GLY17 3.5 14.6 1.0 C5 B:GDP204 3.7 21.7 1.0 C8 B:GDP204 3.8 22.8 1.0 O6 B:GDP204 3.8 22.6 1.0 CG B:ASN117 3.8 18.5 1.0 CB B:ASN117 3.9 17.6 1.0 N B:LEU21 4.0 16.1 1.0 ND2 B:ASN117 4.0 17.7 1.0 OD1 B:ASN117 4.1 20.4 1.0 N B:ALA161 4.1 19.3 1.0 CB B:LEU21 4.2 16.2 1.0 C6 B:GDP204 4.2 23.7 1.0 CB B:CYS20 4.2 19.0 1.0 CA B:ALA161 4.4 19.4 1.0 CA B:LEU21 4.4 16.1 1.0 C B:CYS20 4.6 17.2 1.0 O B:CYS16 4.6 17.0 1.0 N B:LYS118 4.6 20.7 1.0 N B:GLY17 4.7 15.6 1.0 CG B:LYS118 4.7 23.4 1.0 N B:LYS18 4.8 15.3 1.0 CA B:CYS20 4.9 18.0 1.0 CA B:ASN117 4.9 18.2 1.0 N9 B:GDP204 4.9 24.8 1.0 C4 B:GDP204 5.0 23.4 1.0

Z.Yan, S.Ma, Y.Zhang, L.Ma, F.Wang, J.Li, L.Miao. Crystallization and Preliminary X-Ray Crystallographic Analysis of A Small Gtpase Rhoa To Be Published.