Magnesium in PDB 5hc9: Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA

All present enzymatic activity of Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA:
2.7.7.72;

The structure of Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA, PDB code: 5hc9 was solved by S.Yamashita, K.Tomita, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	98.180, 96.190, 108.580, 90.00, 110.30, 90.00
R / Rfree (%)	21.5 / 26.4

The binding sites of Magnesium atom in the Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA (pdb code 5hc9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA, PDB code: 5hc9:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1001 b:62.5 occ:1.00 OD2 A:ASP99 2.2 0.3 1.0 OD1 A:ASP57 2.3 69.7 1.0 CG A:ASP57 3.3 84.0 1.0 CG A:ASP99 3.4 94.6 1.0 OD1 A:ASP55 3.5 80.2 1.0 OD2 A:ASP57 3.5 74.0 1.0 CB A:ASP99 3.9 88.1 1.0 CB A:ASP55 4.3 0.5 1.0 CG A:ASP55 4.4 94.0 1.0 OD1 A:ASP99 4.4 62.0 1.0 O A:ILE56 4.7 81.5 1.0 CB A:ASP57 4.7 69.1 1.0 C A:ILE56 4.9 72.7 1.0

Magnesium binding site 2 out of 2 in the Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Thermotoga Maritima Cca-Adding Enzyme Complexed with TRNA_CCA within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1001 b:71.7 occ:1.00 OD2 B:ASP99 2.2 0.7 1.0 OD2 B:ASP57 2.6 1.0 1.0 OD1 B:ASP57 3.0 93.6 1.0 CG B:ASP57 3.1 88.3 1.0 CG B:ASP99 3.3 87.1 1.0 NH2 B:ARG97 3.5 0.3 1.0 OD1 B:ASP55 3.6 69.8 1.0 CB B:ASP99 3.9 79.9 1.0 OD1 B:ASP99 4.3 50.1 1.0 CZ B:ARG97 4.5 0.1 1.0 CG B:ASP55 4.6 71.0 1.0 CB B:ASP57 4.6 79.8 1.0 CB B:ASP55 4.7 0.4 1.0 NE B:ARG97 4.9 84.9 1.0

S.Yamashita, K.Tomita. Mechanism of 3'-Matured Trna Discrimination From 3'-Immature Trna By Class-II Cca-Adding Enzyme Structure V. 24 918 2016.
ISSN: ISSN 0969-2126
PubMed: 27133023
DOI: 10.1016/J.STR.2016.03.022