Magnesium in PDB 5huh: Crystal Structure of Nade From Streptococcus Pyogenes

All present enzymatic activity of Crystal Structure of Nade From Streptococcus Pyogenes:
6.3.1.5;

The structure of Crystal Structure of Nade From Streptococcus Pyogenes, PDB code: 5huh was solved by W.T.Booth, M.Chruszcz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.72 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.521, 93.104, 128.569, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 22.6

The binding sites of Magnesium atom in the Crystal Structure of Nade From Streptococcus Pyogenes (pdb code 5huh). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Nade From Streptococcus Pyogenes, PDB code: 5huh:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Nade From Streptococcus Pyogenes


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Nade From Streptococcus Pyogenes within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg308 b:19.4 occ:1.00 OD2 A:ASP60 2.0 20.6 1.0 O1 A:SO4301 2.1 33.7 1.0 OE2 A:GLU173 2.1 25.7 1.0 O A:HOH412 2.2 21.3 1.0 O A:HOH463 2.2 13.7 1.0 O A:HOH461 2.2 26.1 1.0 CG A:ASP60 3.1 21.9 1.0 CD A:GLU173 3.2 25.2 1.0 S A:SO4301 3.4 33.5 1.0 OE1 A:GLU173 3.5 25.5 1.0 O4 A:SO4301 3.6 33.2 1.0 CB A:ASP60 3.7 21.2 1.0 O2 A:SO4306 3.9 64.2 1.0 O A:HOH413 4.1 26.7 1.0 OD1 A:ASP60 4.2 21.4 1.0 O2 A:SO4301 4.3 37.1 1.0 NE2 A:HIS170 4.3 23.0 1.0 O3 A:SO4301 4.3 33.5 1.0 NZ A:LYS197 4.5 23.5 1.0 CG A:GLU173 4.5 25.2 1.0 OG1 A:THR168 4.6 21.3 1.0 CD2 A:HIS170 4.8 23.2 1.0 O A:HOH416 5.0 32.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Nade From Streptococcus Pyogenes


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Nade From Streptococcus Pyogenes within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg307 b:32.6 occ:1.00 O4 B:SO4302 1.9 39.7 1.0 O B:HOH455 2.1 30.2 1.0 OD2 B:ASP60 2.2 22.2 1.0 O B:HOH415 2.2 26.7 1.0 OE2 B:GLU173 2.3 31.3 1.0 O B:HOH464 2.4 23.5 1.0 CG B:ASP60 3.3 23.9 1.0 S B:SO4302 3.3 39.8 1.0 CD B:GLU173 3.3 31.3 1.0 O2 B:SO4302 3.6 38.2 1.0 OE1 B:GLU173 3.7 32.1 1.0 CB B:ASP60 3.8 24.0 1.0 O2 B:SO4303 3.8 73.8 1.0 O3 B:SO4302 4.1 37.2 1.0 O1 B:SO4302 4.2 34.5 1.0 O B:HOH417 4.3 25.5 1.0 OD1 B:ASP60 4.4 24.5 1.0 NE2 B:HIS170 4.5 27.8 1.0 CG B:GLU173 4.6 29.5 1.0 NZ B:LYS197 4.6 31.0 1.0 O B:HOH428 4.6 37.0 1.0 OG1 B:THR168 4.7 25.0 1.0

W.T.Booth, T.L.Morris, D.P.Mysona, M.J.Shah, L.K.Taylor, T.W.Karlin, K.Clary, K.A.Majorek, L.R.Offermann, M.Chruszcz. Streptococcus Pyogenes Quinolinate-Salvage Pathway-Structural and Functional Studies of Quinolinate Phosphoribosyl Transferase and NH3 -Dependent Nad(+) Synthetase. Febs J. V. 284 2425 2017.
ISSN: ISSN 1742-4658
PubMed: 28618168
DOI: 10.1111/FEBS.14136