Magnesium in PDB 5hxt: Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp

All present enzymatic activity of Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp:
2.5.1.92;

The structure of Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp, PDB code: 5hxt was solved by C.C.Lee, Y.T.Chan, A.H.J.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.15
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.981, 66.321, 128.939, 90.00, 90.00, 90.00
R / Rfree (%)	19.7 / 24.9

The binding sites of Magnesium atom in the Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp (pdb code 5hxt). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp, PDB code: 5hxt:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:33.6 occ:1.00 O5 A:DST402 2.0 34.6 1.0 O7 A:DST402 2.1 35.8 1.0 O A:HOH579 2.2 29.4 1.0 OD1 A:ASP86 2.3 18.9 1.0 O A:HOH548 2.3 27.7 1.0 O9 A:IPR403 2.6 38.8 1.0 P1 A:DST402 3.1 35.0 1.0 CG A:ASP86 3.2 17.3 1.0 P3 A:DST402 3.4 36.0 1.0 OD2 A:ASP86 3.4 17.7 1.0 O4 A:DST402 3.4 32.6 1.0 O2 A:DST402 3.7 35.3 1.0 NH2 A:ARG137 3.8 36.0 1.0 P7 A:IPR403 4.0 44.5 1.0 O A:HOH572 4.2 32.7 1.0 O8 A:IPR403 4.3 46.6 1.0 S9 A:DST402 4.3 36.7 1.0 O12 A:IPR403 4.3 32.4 0.6 C10 A:DST402 4.4 39.0 1.0 O6 A:DST402 4.4 31.2 1.0 O A:HOH529 4.4 37.5 1.0 O8 A:DST402 4.5 36.0 1.0 CB A:ASP86 4.6 16.4 1.0 O A:HOH508 4.6 40.0 1.0 O14 A:IPR403 4.7 34.2 0.6 N A:GLY87 4.7 15.3 1.0 O6 A:IPR403 4.8 45.3 1.0 C11 A:DST402 4.8 40.3 1.0 C5 A:IPR403 4.9 41.6 1.0 P11 A:IPR403 5.0 34.0 0.6 OD2 A:ASP253 5.0 27.5 1.0 NH2 A:ARG249 5.0 11.8 1.0 CZ A:ARG137 5.0 35.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M, E75A and H103Y Mutants) Complexed with Ipp and Dmspp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:42.6 occ:1.00 O4 B:DST402 2.1 47.7 1.0 O8 B:DST402 2.3 46.9 1.0 OD1 B:ASP86 2.4 29.8 1.0 O13 B:IPR403 2.8 64.7 1.0 CG B:ASP86 3.1 28.4 1.0 OD2 B:ASP86 3.1 28.1 1.0 P1 B:DST402 3.4 45.2 1.0 P3 B:DST402 3.6 46.7 1.0 O6 B:IPR403 3.7 61.6 1.0 O9 B:IPR403 3.7 73.6 1.0 O6 B:DST402 3.9 46.8 1.0 NH2 B:ARG137 3.9 49.0 1.0 O2 B:DST402 4.0 47.5 1.0 P11 B:IPR403 4.1 63.8 1.0 NH2 B:ARG90 4.1 48.6 1.0 P7 B:IPR403 4.2 69.4 1.0 C5 B:IPR403 4.2 55.3 1.0 C10 B:DST402 4.5 47.2 1.0 CB B:ASP86 4.5 27.0 1.0 NH2 B:ARG249 4.5 21.4 1.0 O10 B:IPR403 4.6 67.2 1.0 O5 B:DST402 4.6 46.0 1.0 S9 B:DST402 4.7 44.7 1.0 O14 B:IPR403 4.7 62.1 1.0 O7 B:DST402 4.7 45.8 1.0 N B:GLY87 4.9 28.9 1.0

Y.T.Chan, T.P.Ko, S.H.Yao, Y.W.Chen, C.C.Lee, A.H.J.Wang. Crystal Structure and Potential Head-to-Middle Condensation Function of A Z,Z-Farnesyl Diphosphate Synthase Acs Omega V. 2 930 2017.
ISSN: ESSN 2470-1343
DOI: 10.1021/ACSOMEGA.6B00562