Magnesium in PDB 5imn: Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Enzymatic activity of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
All present enzymatic activity of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium:
4.2.3.9;
Protein crystallography data
The structure of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium, PDB code: 5imn
was solved by
M.Chen,
D.W.Christianson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
47.53 /
2.53
|
Space group
|
P 31 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
124.299,
124.299,
202.631,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
19.5 /
25.4
|
Magnesium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
12;
Binding sites:
The binding sites of Magnesium atom in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
(pdb code 5imn). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the
Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium, PDB code: 5imn:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Magnesium binding site 1 out
of 12 in 5imn
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Magnesium Binding Sites List in 5imn
Magnesium binding site 1 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg402
b:41.7
occ:1.00
|
O2
|
A:POP401
|
2.0
|
37.3
|
1.0
|
O
|
A:HOH509
|
2.1
|
37.3
|
1.0
|
O
|
A:HOH546
|
2.1
|
33.1
|
1.0
|
OD1
|
A:ASP84
|
2.2
|
40.5
|
1.0
|
O
|
A:HOH519
|
2.2
|
41.1
|
1.0
|
O
|
A:HOH533
|
2.3
|
42.1
|
1.0
|
CG
|
A:ASP84
|
3.0
|
40.0
|
1.0
|
OD2
|
A:ASP84
|
3.0
|
32.9
|
1.0
|
MG
|
A:MG403
|
3.0
|
39.3
|
1.0
|
P1
|
A:POP401
|
3.2
|
38.5
|
1.0
|
O1
|
A:POP401
|
3.5
|
40.0
|
1.0
|
O
|
A:HOH551
|
3.8
|
43.2
|
1.0
|
OD1
|
A:ASP172
|
4.0
|
38.2
|
1.0
|
O3
|
A:POP401
|
4.1
|
31.3
|
1.0
|
OD2
|
A:ASP172
|
4.2
|
42.5
|
1.0
|
NH2
|
A:ARG169
|
4.3
|
40.5
|
1.0
|
O
|
A:HOH508
|
4.4
|
48.8
|
1.0
|
NE2
|
A:GLN151
|
4.4
|
38.0
|
1.0
|
O
|
A:POP401
|
4.4
|
44.4
|
1.0
|
O
|
A:HOH531
|
4.4
|
38.5
|
1.0
|
CB
|
A:ASP84
|
4.5
|
36.8
|
1.0
|
CG
|
A:ASP172
|
4.5
|
40.8
|
1.0
|
O
|
A:HOH581
|
4.6
|
43.5
|
1.0
|
O
|
A:HOH502
|
4.7
|
37.5
|
1.0
|
O6
|
A:POP401
|
4.8
|
36.8
|
1.0
|
O
|
A:ASP172
|
4.8
|
41.0
|
1.0
|
CG
|
A:GLN151
|
4.9
|
40.1
|
1.0
|
|
Magnesium binding site 2 out
of 12 in 5imn
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Magnesium Binding Sites List in 5imn
Magnesium binding site 2 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg403
b:39.3
occ:1.00
|
O
|
A:HOH502
|
2.0
|
37.5
|
1.0
|
O
|
A:HOH531
|
2.0
|
38.5
|
1.0
|
OD2
|
A:ASP84
|
2.1
|
32.9
|
1.0
|
O2
|
A:POP401
|
2.1
|
37.3
|
1.0
|
O
|
A:HOH519
|
2.1
|
41.1
|
1.0
|
O6
|
A:POP401
|
2.3
|
36.8
|
1.0
|
MG
|
A:MG402
|
3.0
|
41.7
|
1.0
|
P1
|
A:POP401
|
3.2
|
38.5
|
1.0
|
CG
|
A:ASP84
|
3.2
|
40.0
|
1.0
|
P2
|
A:POP401
|
3.5
|
41.7
|
1.0
|
O
|
A:POP401
|
3.6
|
44.4
|
1.0
|
OE2
|
A:GLU88
|
3.7
|
56.2
|
1.0
|
OD1
|
A:ASP84
|
3.7
|
40.5
|
1.0
|
O3
|
A:POP401
|
3.7
|
31.3
|
1.0
|
NZ
|
A:LYS220
|
4.0
|
33.1
|
1.0
|
O5
|
A:POP401
|
4.2
|
36.2
|
1.0
|
O
|
A:HOH533
|
4.2
|
42.1
|
1.0
|
O
|
A:HOH551
|
4.3
|
43.2
|
1.0
|
OE2
|
A:GLU221
|
4.4
|
44.6
|
1.0
|
NH2
|
A:ARG308
|
4.4
|
41.9
|
1.0
|
O1
|
A:POP401
|
4.5
|
40.0
|
1.0
|
CB
|
A:ASP84
|
4.5
|
36.8
|
1.0
|
O
|
A:HOH509
|
4.6
|
37.3
|
1.0
|
O4
|
A:POP401
|
4.6
|
39.5
|
1.0
|
CD
|
A:GLU88
|
4.8
|
55.7
|
1.0
|
O
|
A:HOH517
|
4.8
|
38.9
|
1.0
|
O
|
A:ASP84
|
4.8
|
40.7
|
1.0
|
O
|
A:HOH581
|
4.9
|
43.5
|
1.0
|
O
|
A:HOH546
|
4.9
|
33.1
|
1.0
|
MG
|
A:MG404
|
4.9
|
38.4
|
1.0
|
|
Magnesium binding site 3 out
of 12 in 5imn
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Magnesium Binding Sites List in 5imn
Magnesium binding site 3 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg404
b:38.4
occ:1.00
|
OE2
|
A:GLU221
|
1.9
|
44.6
|
1.0
|
O5
|
A:POP401
|
2.0
|
36.2
|
1.0
|
O
|
A:HOH505
|
2.1
|
39.0
|
1.0
|
OG
|
A:SER217
|
2.1
|
36.5
|
1.0
|
OD1
|
A:ASN213
|
2.2
|
40.9
|
1.0
|
O3
|
A:POP401
|
2.3
|
31.3
|
1.0
|
CD
|
A:GLU221
|
3.0
|
48.8
|
1.0
|
CB
|
A:SER217
|
3.1
|
36.1
|
1.0
|
P2
|
A:POP401
|
3.3
|
41.7
|
1.0
|
CG
|
A:ASN213
|
3.3
|
40.5
|
1.0
|
P1
|
A:POP401
|
3.4
|
38.5
|
1.0
|
O
|
A:POP401
|
3.5
|
44.4
|
1.0
|
OE1
|
A:GLU221
|
3.6
|
42.6
|
1.0
|
ND2
|
A:ASN213
|
3.7
|
41.1
|
1.0
|
O
|
A:HOH531
|
3.8
|
38.5
|
1.0
|
O6
|
A:POP401
|
4.0
|
36.8
|
1.0
|
O
|
A:ASN213
|
4.1
|
36.2
|
1.0
|
O
|
A:HOH581
|
4.1
|
43.5
|
1.0
|
CG
|
A:GLU221
|
4.1
|
38.3
|
1.0
|
NH1
|
A:ARG169
|
4.3
|
40.0
|
1.0
|
C
|
A:ASN213
|
4.4
|
38.9
|
1.0
|
O4
|
A:POP401
|
4.4
|
39.5
|
1.0
|
O2
|
A:POP401
|
4.4
|
37.3
|
1.0
|
CA
|
A:SER217
|
4.4
|
32.0
|
1.0
|
OD1
|
A:ASP214
|
4.4
|
37.8
|
1.0
|
O1
|
A:POP401
|
4.5
|
40.0
|
1.0
|
CB
|
A:ASN213
|
4.6
|
35.8
|
1.0
|
N
|
A:ASP214
|
4.6
|
35.3
|
1.0
|
CA
|
A:ASP214
|
4.7
|
36.4
|
1.0
|
O
|
A:SER217
|
4.8
|
41.7
|
1.0
|
C
|
A:SER217
|
4.8
|
39.8
|
1.0
|
O
|
A:HOH510
|
4.9
|
36.5
|
1.0
|
MG
|
A:MG403
|
4.9
|
39.3
|
1.0
|
NH2
|
A:ARG169
|
5.0
|
40.5
|
1.0
|
|
Magnesium binding site 4 out
of 12 in 5imn
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Magnesium Binding Sites List in 5imn
Magnesium binding site 4 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg402
b:53.6
occ:1.00
|
OD1
|
B:ASP84
|
2.2
|
46.0
|
1.0
|
O3
|
B:POP401
|
2.2
|
43.2
|
1.0
|
O
|
B:HOH540
|
2.2
|
46.2
|
1.0
|
O
|
B:HOH539
|
2.3
|
46.4
|
1.0
|
O
|
B:HOH554
|
2.3
|
57.3
|
1.0
|
O
|
B:HOH529
|
2.4
|
54.3
|
1.0
|
MG
|
B:MG403
|
3.2
|
45.6
|
1.0
|
CG
|
B:ASP84
|
3.3
|
50.1
|
1.0
|
P1
|
B:POP401
|
3.4
|
41.6
|
1.0
|
O2
|
B:POP401
|
3.6
|
47.1
|
1.0
|
OD2
|
B:ASP84
|
3.7
|
49.1
|
1.0
|
OD2
|
B:ASP172
|
3.8
|
47.7
|
1.0
|
OD1
|
B:ASP172
|
3.8
|
48.7
|
1.0
|
O
|
B:HOH515
|
4.0
|
47.3
|
1.0
|
O1
|
B:POP401
|
4.2
|
42.2
|
1.0
|
NH2
|
B:ARG169
|
4.2
|
44.3
|
1.0
|
O
|
B:HOH555
|
4.2
|
50.9
|
1.0
|
CG
|
B:ASP172
|
4.2
|
54.7
|
1.0
|
O
|
B:HOH511
|
4.5
|
39.7
|
1.0
|
NE2
|
B:GLN151
|
4.5
|
42.1
|
1.0
|
CB
|
B:ASP84
|
4.6
|
47.9
|
1.0
|
O
|
B:ASP172
|
4.6
|
42.1
|
1.0
|
O
|
B:POP401
|
4.6
|
47.0
|
1.0
|
NH2
|
B:ARG156
|
4.8
|
56.2
|
1.0
|
O
|
B:HOH510
|
4.8
|
44.3
|
1.0
|
CG
|
B:GLN151
|
4.9
|
45.8
|
1.0
|
O5
|
B:POP401
|
5.0
|
44.6
|
1.0
|
|
Magnesium binding site 5 out
of 12 in 5imn
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Magnesium Binding Sites List in 5imn
Magnesium binding site 5 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg403
b:45.6
occ:1.00
|
O
|
B:HOH511
|
2.0
|
39.7
|
1.0
|
O
|
B:HOH510
|
2.0
|
44.3
|
1.0
|
O3
|
B:POP401
|
2.1
|
43.2
|
1.0
|
O
|
B:HOH529
|
2.3
|
54.3
|
1.0
|
O5
|
B:POP401
|
2.3
|
44.6
|
1.0
|
OD2
|
B:ASP84
|
2.4
|
49.1
|
1.0
|
CG
|
B:ASP84
|
3.2
|
50.1
|
1.0
|
MG
|
B:MG402
|
3.2
|
53.6
|
1.0
|
OD1
|
B:ASP84
|
3.3
|
46.0
|
1.0
|
P1
|
B:POP401
|
3.4
|
41.6
|
1.0
|
P2
|
B:POP401
|
3.5
|
46.3
|
1.0
|
NZ
|
B:LYS220
|
3.7
|
56.1
|
1.0
|
OE2
|
B:GLU88
|
3.7
|
69.4
|
1.0
|
O
|
B:POP401
|
3.7
|
47.0
|
1.0
|
O1
|
B:POP401
|
3.9
|
42.2
|
1.0
|
O
|
B:HOH515
|
4.0
|
47.3
|
1.0
|
OE2
|
B:GLU221
|
4.1
|
52.6
|
1.0
|
O
|
B:HOH539
|
4.2
|
46.4
|
1.0
|
O4
|
B:POP401
|
4.3
|
43.2
|
1.0
|
O
|
B:ASP84
|
4.5
|
51.1
|
1.0
|
O2
|
B:POP401
|
4.6
|
47.1
|
1.0
|
NH2
|
B:ARG308
|
4.6
|
46.2
|
1.0
|
CD
|
B:GLU88
|
4.6
|
78.0
|
1.0
|
CB
|
B:ASP84
|
4.7
|
47.9
|
1.0
|
O
|
B:HOH555
|
4.7
|
50.9
|
1.0
|
O6
|
B:POP401
|
4.7
|
38.8
|
1.0
|
MG
|
B:MG404
|
4.8
|
43.5
|
1.0
|
CE
|
B:LYS220
|
4.8
|
53.2
|
1.0
|
OD1
|
B:ASP85
|
4.9
|
61.5
|
1.0
|
O
|
B:HOH540
|
4.9
|
46.2
|
1.0
|
C
|
B:ASP84
|
5.0
|
53.5
|
1.0
|
CD
|
B:GLU221
|
5.0
|
48.5
|
1.0
|
O
|
B:HOH554
|
5.0
|
57.3
|
1.0
|
|
Magnesium binding site 6 out
of 12 in 5imn
Go back to
Magnesium Binding Sites List in 5imn
Magnesium binding site 6 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg404
b:43.5
occ:1.00
|
OE2
|
B:GLU221
|
2.1
|
52.6
|
1.0
|
O
|
B:HOH536
|
2.1
|
39.9
|
1.0
|
OD1
|
B:ASN213
|
2.2
|
39.6
|
1.0
|
O1
|
B:POP401
|
2.2
|
42.2
|
1.0
|
O4
|
B:POP401
|
2.3
|
43.2
|
1.0
|
OG
|
B:SER217
|
2.3
|
40.8
|
1.0
|
CD
|
B:GLU221
|
3.0
|
48.5
|
1.0
|
CG
|
B:ASN213
|
3.3
|
40.4
|
1.0
|
P1
|
B:POP401
|
3.3
|
41.6
|
1.0
|
CB
|
B:SER217
|
3.3
|
43.9
|
1.0
|
P2
|
B:POP401
|
3.4
|
46.3
|
1.0
|
O
|
B:POP401
|
3.4
|
47.0
|
1.0
|
OE1
|
B:GLU221
|
3.5
|
45.3
|
1.0
|
O
|
B:HOH515
|
3.5
|
47.3
|
1.0
|
ND2
|
B:ASN213
|
3.7
|
37.3
|
1.0
|
O
|
B:HOH511
|
3.9
|
39.7
|
1.0
|
O5
|
B:POP401
|
4.0
|
44.6
|
1.0
|
O
|
B:ASN213
|
4.0
|
40.9
|
1.0
|
O3
|
B:POP401
|
4.1
|
43.2
|
1.0
|
NH1
|
B:ARG169
|
4.2
|
36.9
|
1.0
|
CG
|
B:GLU221
|
4.3
|
39.6
|
1.0
|
C
|
B:ASN213
|
4.4
|
39.4
|
1.0
|
O2
|
B:POP401
|
4.4
|
47.1
|
1.0
|
CB
|
B:ASN213
|
4.6
|
34.9
|
1.0
|
O6
|
B:POP401
|
4.6
|
38.8
|
1.0
|
OD1
|
B:ASP214
|
4.6
|
43.8
|
1.0
|
NZ
|
B:LYS220
|
4.7
|
56.1
|
1.0
|
CA
|
B:SER217
|
4.7
|
43.7
|
1.0
|
NH2
|
B:ARG169
|
4.8
|
44.3
|
1.0
|
CA
|
B:ASP214
|
4.8
|
34.4
|
1.0
|
MG
|
B:MG403
|
4.8
|
45.6
|
1.0
|
N
|
B:ASP214
|
4.8
|
35.9
|
1.0
|
CZ
|
B:ARG169
|
4.9
|
41.0
|
1.0
|
|
Magnesium binding site 7 out
of 12 in 5imn
Go back to
Magnesium Binding Sites List in 5imn
Magnesium binding site 7 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 7 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg402
b:68.5
occ:1.00
|
O
|
C:HOH520
|
1.8
|
67.3
|
1.0
|
O
|
C:HOH514
|
2.0
|
55.8
|
1.0
|
O3
|
C:POP401
|
2.1
|
59.8
|
1.0
|
OD1
|
C:ASP84
|
2.2
|
73.5
|
1.0
|
O
|
C:HOH510
|
2.4
|
62.2
|
1.0
|
O
|
C:HOH533
|
2.6
|
58.1
|
1.0
|
CG
|
C:ASP84
|
3.1
|
64.7
|
1.0
|
P1
|
C:POP401
|
3.2
|
57.5
|
1.0
|
MG
|
C:MG403
|
3.3
|
53.8
|
1.0
|
OD2
|
C:ASP84
|
3.4
|
71.5
|
1.0
|
O2
|
C:POP401
|
3.4
|
64.8
|
1.0
|
O1
|
C:POP401
|
3.7
|
69.8
|
1.0
|
O
|
C:HOH516
|
4.1
|
61.6
|
1.0
|
OD2
|
C:ASP172
|
4.2
|
54.2
|
1.0
|
CB
|
C:ASP84
|
4.4
|
63.1
|
1.0
|
OD1
|
C:ASP172
|
4.4
|
52.4
|
1.0
|
O5
|
C:POP401
|
4.5
|
53.2
|
1.0
|
NE2
|
C:GLN151
|
4.6
|
58.1
|
1.0
|
O
|
C:POP401
|
4.6
|
65.8
|
1.0
|
CG
|
C:ASP172
|
4.7
|
58.2
|
1.0
|
O
|
C:ASP172
|
4.7
|
55.5
|
1.0
|
NH2
|
C:ARG169
|
4.8
|
85.3
|
1.0
|
CAH
|
C:JF1405
|
4.9
|
69.3
|
1.0
|
|
Magnesium binding site 8 out
of 12 in 5imn
Go back to
Magnesium Binding Sites List in 5imn
Magnesium binding site 8 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 8 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg403
b:53.8
occ:1.00
|
O
|
C:HOH516
|
1.9
|
61.6
|
1.0
|
O5
|
C:POP401
|
2.1
|
53.2
|
1.0
|
O3
|
C:POP401
|
2.3
|
59.8
|
1.0
|
OD2
|
C:ASP84
|
2.3
|
71.5
|
1.0
|
O
|
C:HOH510
|
2.4
|
62.2
|
1.0
|
NZ
|
C:LYS220
|
3.0
|
60.0
|
1.0
|
CG
|
C:ASP84
|
3.1
|
64.7
|
1.0
|
OD1
|
C:ASP84
|
3.2
|
73.5
|
1.0
|
OE2
|
C:GLU88
|
3.3
|
67.7
|
1.0
|
MG
|
C:MG402
|
3.3
|
68.5
|
1.0
|
P2
|
C:POP401
|
3.5
|
61.4
|
1.0
|
P1
|
C:POP401
|
3.6
|
57.5
|
1.0
|
NH2
|
C:ARG308
|
3.9
|
59.2
|
1.0
|
O
|
C:POP401
|
4.0
|
65.8
|
1.0
|
O1
|
C:POP401
|
4.0
|
69.8
|
1.0
|
O4
|
C:POP401
|
4.2
|
73.6
|
1.0
|
OE2
|
C:GLU221
|
4.2
|
67.0
|
1.0
|
OD1
|
C:ASP85
|
4.2
|
71.0
|
1.0
|
CE
|
C:LYS220
|
4.4
|
63.2
|
1.0
|
CD
|
C:GLU88
|
4.4
|
71.7
|
1.0
|
CB
|
C:ASP84
|
4.4
|
63.1
|
1.0
|
O6
|
C:POP401
|
4.6
|
52.7
|
1.0
|
O
|
C:ASP84
|
4.6
|
63.3
|
1.0
|
O2
|
C:POP401
|
4.8
|
64.8
|
1.0
|
MG
|
C:MG404
|
4.8
|
55.9
|
1.0
|
O
|
C:HOH520
|
4.8
|
67.3
|
1.0
|
C
|
C:ASP84
|
4.8
|
61.6
|
1.0
|
O
|
C:HOH514
|
4.9
|
55.8
|
1.0
|
CG
|
C:GLU88
|
4.9
|
61.5
|
1.0
|
|
Magnesium binding site 9 out
of 12 in 5imn
Go back to
Magnesium Binding Sites List in 5imn
Magnesium binding site 9 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 9 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg404
b:55.9
occ:1.00
|
OE2
|
C:GLU221
|
1.9
|
67.0
|
1.0
|
O4
|
C:POP401
|
2.1
|
73.6
|
1.0
|
OD1
|
C:ASN213
|
2.2
|
56.2
|
1.0
|
OG
|
C:SER217
|
2.5
|
62.0
|
1.0
|
O
|
C:HOH504
|
2.5
|
63.6
|
1.0
|
O1
|
C:POP401
|
2.5
|
69.8
|
1.0
|
O
|
C:POP401
|
2.8
|
65.8
|
1.0
|
CD
|
C:GLU221
|
2.9
|
71.4
|
1.0
|
P2
|
C:POP401
|
3.0
|
61.4
|
1.0
|
CG
|
C:ASN213
|
3.2
|
57.8
|
1.0
|
P1
|
C:POP401
|
3.2
|
57.5
|
1.0
|
CB
|
C:SER217
|
3.3
|
63.3
|
1.0
|
OE1
|
C:GLU221
|
3.4
|
70.6
|
1.0
|
ND2
|
C:ASN213
|
3.7
|
59.4
|
1.0
|
O5
|
C:POP401
|
3.7
|
53.2
|
1.0
|
NH2
|
C:ARG169
|
3.8
|
85.3
|
1.0
|
CG
|
C:GLU221
|
4.0
|
75.9
|
1.0
|
O
|
C:HOH516
|
4.0
|
61.6
|
1.0
|
O3
|
C:POP401
|
4.1
|
59.8
|
1.0
|
O6
|
C:POP401
|
4.2
|
52.7
|
1.0
|
O
|
C:ASN213
|
4.2
|
58.8
|
1.0
|
O2
|
C:POP401
|
4.4
|
64.8
|
1.0
|
NZ
|
C:LYS220
|
4.6
|
60.0
|
1.0
|
C
|
C:ASN213
|
4.6
|
60.1
|
1.0
|
CB
|
C:ASN213
|
4.6
|
57.1
|
1.0
|
CA
|
C:SER217
|
4.7
|
55.9
|
1.0
|
CZ
|
C:ARG169
|
4.8
|
68.6
|
1.0
|
MG
|
C:MG403
|
4.8
|
53.8
|
1.0
|
NH1
|
C:ARG169
|
4.8
|
61.8
|
1.0
|
OD1
|
C:ASP214
|
4.8
|
68.0
|
1.0
|
CE1
|
C:TYR309
|
4.9
|
59.4
|
1.0
|
O
|
C:SER217
|
5.0
|
67.2
|
1.0
|
OH
|
C:TYR309
|
5.0
|
62.5
|
1.0
|
N
|
C:ASP214
|
5.0
|
60.2
|
1.0
|
|
Magnesium binding site 10 out
of 12 in 5imn
Go back to
Magnesium Binding Sites List in 5imn
Magnesium binding site 10 out
of 12 in the Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 10 of Crystal Structure of N299A/S303A Aspergillus Terreus Aristolochene Synthase Complexed with (1S,8S,9AR)-1,9A-Dimethyl-8-(Prop-1-En-2-Yl) Decahydroquinolizin-5-Ium within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg402
b:47.4
occ:1.00
|
O
|
D:HOH515
|
2.0
|
54.5
|
1.0
|
O3
|
D:POP401
|
2.1
|
66.4
|
1.0
|
OD1
|
D:ASP84
|
2.1
|
61.8
|
1.0
|
O
|
D:HOH501
|
2.2
|
48.6
|
1.0
|
O
|
D:HOH523
|
2.3
|
59.7
|
1.0
|
O
|
D:HOH511
|
2.3
|
52.8
|
1.0
|
CG
|
D:ASP84
|
2.9
|
57.5
|
1.0
|
OD2
|
D:ASP84
|
2.9
|
54.9
|
1.0
|
MG
|
D:MG403
|
2.9
|
51.3
|
1.0
|
O2
|
D:POP401
|
3.0
|
50.8
|
1.0
|
P1
|
D:POP401
|
3.1
|
54.6
|
1.0
|
O
|
D:HOH527
|
4.0
|
58.1
|
1.0
|
O1
|
D:POP401
|
4.0
|
60.9
|
1.0
|
O
|
D:HOH512
|
4.0
|
60.4
|
1.0
|
O
|
D:HOH520
|
4.1
|
51.1
|
1.0
|
OD2
|
D:ASP172
|
4.2
|
58.8
|
1.0
|
CB
|
D:ASP84
|
4.3
|
56.3
|
1.0
|
O5
|
D:POP401
|
4.4
|
53.6
|
1.0
|
O
|
D:POP401
|
4.4
|
52.0
|
1.0
|
O
|
D:HOH508
|
4.5
|
55.8
|
1.0
|
NH2
|
D:ARG169
|
4.6
|
58.4
|
1.0
|
OD1
|
D:ASP172
|
4.6
|
60.4
|
1.0
|
NE2
|
D:GLN151
|
4.7
|
62.7
|
1.0
|
CG
|
D:ASP172
|
4.8
|
61.8
|
1.0
|
|
Reference:
M.Chen,
W.K.Chou,
N.Al-Lami,
J.A.Faraldos,
R.K.Allemann,
D.E.Cane,
D.W.Christianson.
Probing the Role of Active Site Water in the Sesquiterpene Cyclization Reaction Catalyzed By Aristolochene Synthase. Biochemistry V. 55 2864 2016.
ISSN: ISSN 0006-2960
PubMed: 27172425
DOI: 10.1021/ACS.BIOCHEM.6B00343
Page generated: Sun Sep 29 16:45:05 2024
|