Magnesium in PDB 5jb2: Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution.

The structure of Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution., PDB code: 5jb2 was solved by S.Cusack, E.Uchikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	75.93 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.140, 96.580, 122.860, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 24.5

The structure of Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution. also contains other interesting chemical elements:

Fluorine	(F)	4 atoms
Aluminium	(Al)	1 atom
Zinc	(Zn)	1 atom

The binding sites of Magnesium atom in the Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution. (pdb code 5jb2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution., PDB code: 5jb2:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg704 b:17.7 occ:1.00 F1 A:ALF703 1.9 19.1 1.0 O A:HOH934 2.0 17.2 1.0 O3B A:ADP702 2.1 16.1 1.0 O A:HOH819 2.2 15.7 1.0 O A:HOH828 2.2 16.9 1.0 F4 A:ALF703 2.3 19.6 1.0 AL A:ALF703 2.9 18.5 1.0 PB A:ADP702 3.2 17.1 1.0 O2B A:ADP702 3.3 15.6 1.0 O A:HOH988 3.7 30.2 1.0 O A:HOH817 3.8 17.5 1.0 OE2 A:GLU132 3.8 17.0 1.0 OG1 A:THR31 4.0 18.7 1.0 CA A:GLY442 4.0 18.7 1.0 O3A A:ADP702 4.1 17.1 1.0 OD1 A:ASP131 4.1 17.5 1.0 O2A A:ADP702 4.2 17.3 1.0 F3 A:ALF703 4.2 18.1 1.0 O A:GLY442 4.2 18.3 1.0 O1A A:ADP702 4.3 17.4 1.0 PA A:ADP702 4.4 17.3 1.0 O1B A:ADP702 4.4 16.4 1.0 OD2 A:ASP131 4.4 16.5 1.0 F2 A:ALF703 4.4 17.2 1.0 CE1 A:HIS63 4.5 21.1 1.0 C A:GLY442 4.6 18.3 1.0 CG A:ASP131 4.7 17.4 1.0 CD A:GLU132 4.8 17.2 1.0 NE2 A:HIS63 4.8 20.4 1.0 CE A:LYS30 4.9 18.0 1.0 N A:GLY442 4.9 18.8 1.0 NH2 A:ARG471 4.9 20.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Chicken LGP2 with 5'Ppp 10-Mer Dsrna and Adp- ALF4-MG2+ at 2.2 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ X:Mg101 b:30.8 occ:1.00 OP2 X:G2 2.0 27.6 1.0 O2A X:GTP1 2.0 38.9 1.0 O1B X:GTP1 2.1 39.6 1.0 O X:HOH215 2.2 34.7 1.0 O A:HOH812 2.2 26.1 1.0 O X:HOH224 2.2 36.1 1.0 PB X:GTP1 3.4 43.2 1.0 PA X:GTP1 3.4 35.0 1.0 P X:G2 3.4 26.1 1.0 O3A X:GTP1 3.7 39.8 1.0 NZ A:LYS634 3.8 37.6 1.0 O1G X:GTP1 4.0 53.9 1.0 O3' X:GTP1 4.1 27.7 1.0 C3' X:GTP1 4.2 28.8 1.0 O3B X:GTP1 4.2 48.6 1.0 OP1 X:G2 4.2 28.4 1.0 CE1 A:HIS406 4.2 32.4 1.0 O X:HOH213 4.3 28.5 1.0 O1A X:GTP1 4.3 37.2 1.0 O5' X:G2 4.4 24.3 1.0 O5' X:GTP1 4.4 36.0 1.0 O A:HIS406 4.5 31.5 1.0 O2B X:GTP1 4.6 46.3 1.0 ND1 A:HIS406 4.6 32.4 1.0 C5' X:GTP1 4.7 32.8 1.0 PG X:GTP1 4.7 61.7 1.0 N7 X:G2 4.8 23.4 1.0 C8 X:G2 5.0 22.2 1.0

E.Uchikawa, M.Lethier, H.Malet, J.Brunel, D.Gerlier, S.Cusack. Structural Analysis of Dsrna Binding to Anti-Viral Pattern Recognition Receptors LGP2 and MDA5. Mol.Cell V. 62 586 2016.
ISSN: ISSN 1097-2765
PubMed: 27203181
DOI: 10.1016/J.MOLCEL.2016.04.021