Magnesium in PDB 5jc7: Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution.

The structure of Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution., PDB code: 5jc7 was solved by S.Cusack, E.Uchikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	99.750, 133.400, 138.440, 90.00, 90.00, 90.00
R / Rfree (%)	29.1 / 32.2

The structure of Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution. also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution. (pdb code 5jc7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution., PDB code: 5jc7:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1002 b:37.0 occ:1.00 O1B A:ADP1003 1.9 81.5 1.0 O1A A:ADP1003 2.6 72.7 1.0 PB A:ADP1003 2.8 80.8 1.0 O2B A:ADP1003 2.8 82.3 1.0 OG1 A:THR329 3.2 82.5 1.0 CG2 A:THR329 3.5 73.5 1.0 O3A A:ADP1003 3.6 78.2 1.0 PA A:ADP1003 3.7 77.0 1.0 CB A:THR329 3.9 74.1 1.0 NZ A:LYS328 4.1 71.8 1.0 O3B A:ADP1003 4.1 78.9 1.0 N A:THR329 4.3 74.7 1.0 CG A:LYS328 4.4 78.6 1.0 O5' A:ADP1003 4.6 82.8 1.0 C5' A:ADP1003 4.7 82.8 1.0 CE A:LYS328 4.7 75.0 1.0 CA A:THR329 4.7 72.5 1.0 NE2 A:HIS365 4.8 64.7 1.0 N A:LYS328 4.9 75.0 1.0 O2A A:ADP1003 4.9 75.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Chicken MDA5 with 5'P 24-Mer Dsrna and Adp-MG2+ at 2.75 A Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1002 b:33.1 occ:1.00 O2B B:ADP1003 1.9 75.3 1.0 O2A B:ADP1003 2.1 71.5 1.0 PB B:ADP1003 2.8 77.1 1.0 O3A B:ADP1003 3.1 76.8 1.0 OG1 B:THR329 3.1 75.8 1.0 O1B B:ADP1003 3.1 78.8 1.0 PA B:ADP1003 3.1 76.3 1.0 CG2 B:THR329 3.8 72.7 1.0 CB B:THR329 4.1 73.5 1.0 O5' B:ADP1003 4.2 81.0 1.0 O3B B:ADP1003 4.2 73.0 1.0 O1A B:ADP1003 4.3 74.9 1.0 C5' B:ADP1003 4.4 82.8 1.0 N B:THR329 4.5 75.6 1.0 NZ B:LYS328 4.8 75.3 1.0 CG B:LYS328 4.9 77.5 1.0 CA B:THR329 4.9 73.2 1.0 CA B:GLY327 5.0 75.7 1.0 N B:LYS328 5.0 73.7 1.0

E.Uchikawa, M.Lethier, H.Malet, J.Brunel, D.Gerlier, S.Cusack. Structural Analysis of Dsrna Binding to Anti-Viral Pattern Recognition Receptors LGP2 and MDA5. Mol.Cell V. 62 586 2016.
ISSN: ISSN 1097-2765
PubMed: 27203181
DOI: 10.1016/J.MOLCEL.2016.04.021