Magnesium in PDB 5jch: Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).

The structure of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned)., PDB code: 5jch was solved by S.Cusack, E.Uchikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.080, 139.730, 103.190, 90.00, 110.14, 90.00
R / Rfree (%)	25.6 / 27

The structure of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). (pdb code 5jch). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned)., PDB code: 5jch:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1003 b:53.5 occ:1.00 O3B A:ADP1002 1.9 0.8 1.0 O2A A:ADP1002 2.1 99.8 1.0 PA A:ADP1002 2.6 99.4 1.0 O1A A:ADP1002 2.8 98.5 1.0 OG1 A:THR329 2.8 81.8 1.0 PB A:ADP1002 3.0 0.2 1.0 O3A A:ADP1002 3.2 100.0 1.0 CG2 A:THR329 3.3 80.7 1.0 CB A:THR329 3.6 80.7 1.0 O1B A:ADP1002 3.8 0.8 1.0 O5' A:ADP1002 4.2 95.3 1.0 O2B A:ADP1002 4.3 0.7 1.0 N A:THR329 4.3 81.3 1.0 CA A:THR329 4.6 80.4 1.0 CG A:LYS328 4.9 84.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned).


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Chicken MDA5 with 5'P 10-Mer Dsrna and Adp-MG2+ at 2.95 A Resolution (Untwinned). within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1003 b:46.2 occ:1.00 O3B B:ADP1002 1.9 0.3 1.0 O1A B:ADP1002 2.4 0.5 1.0 PA B:ADP1002 2.9 0.4 1.0 O2A B:ADP1002 2.9 0.4 1.0 PB B:ADP1002 3.0 0.6 1.0 OG1 B:THR329 3.1 84.5 1.0 O3A B:ADP1002 3.3 0.8 1.0 CG2 B:THR329 3.4 83.6 1.0 O2B B:ADP1002 3.4 0.1 1.0 CB B:THR329 3.9 83.0 1.0 O1B B:ADP1002 4.3 0.3 1.0 O5' B:ADP1002 4.5 0.6 1.0 N B:THR329 4.6 81.3 1.0 CA B:THR329 4.9 82.0 1.0 CG B:LYS328 5.0 81.7 1.0

E.Uchikawa, M.Lethier, H.Malet, J.Brunel, D.Gerlier, S.Cusack. Structural Analysis of Dsrna Binding to Anti-Viral Pattern Recognition Receptors LGP2 and MDA5. Mol.Cell V. 62 586 2016.
ISSN: ISSN 1097-2765
PubMed: 27203181
DOI: 10.1016/J.MOLCEL.2016.04.021