Magnesium in PDB 5l0q: Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7.

All present enzymatic activity of Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7.:
3.4.24.81;

The structure of Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7., PDB code: 5l0q was solved by K.Xu, N.Saha, D.B.Nikolov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	125.26 / 2.76
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.326, 141.679, 268.080, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 25.1

The binding sites of Magnesium atom in the Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7. (pdb code 5l0q). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7., PDB code: 5l0q:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg705 b:28.5 occ:1.00 O A:ILE459 2.5 41.5 1.0 OE2 A:GLU469 2.7 38.0 1.0 OD2 A:ASP472 2.7 37.6 1.0 O A:MET464 2.7 41.0 1.0 OE2 A:GLU466 2.7 37.1 1.0 OD1 A:ASN462 2.7 38.7 1.0 OE1 A:GLU469 2.7 45.8 1.0 CD A:GLU469 3.0 44.7 1.0 C A:ILE459 3.4 38.7 1.0 CA A:CYS460 3.6 38.8 1.0 CD A:GLU466 3.7 42.9 1.0 CG A:ASP472 3.8 37.7 1.0 CG A:ASN462 3.8 37.1 1.0 C A:MET464 3.9 36.5 1.0 N A:CYS460 3.9 38.5 1.0 O A:ASN462 4.0 38.7 1.0 N A:ILE459 4.1 40.0 1.0 ND2 A:ASN462 4.2 38.1 1.0 CB A:GLU466 4.2 44.0 1.0 N A:GLY461 4.3 32.7 1.0 CB A:ASP472 4.3 33.0 1.0 CG A:GLU469 4.3 48.1 1.0 O A:HOH817 4.4 36.7 1.0 OE1 A:GLU466 4.4 44.5 1.0 N A:GLU466 4.4 38.8 1.0 CA A:ILE459 4.5 35.1 1.0 CG A:GLU466 4.5 42.9 1.0 C A:CYS460 4.5 34.8 1.0 N A:ASN462 4.6 40.5 1.0 CB A:CYS460 4.6 42.2 1.0 CA A:VAL465 4.6 36.0 1.0 OD1 A:ASP472 4.7 39.0 1.0 N A:VAL465 4.7 31.4 1.0 C A:VAL465 4.7 38.8 1.0 N A:MET464 4.7 30.8 1.0 CA A:MET464 4.8 34.1 1.0 C A:ASN462 4.9 39.5 1.0 CA A:GLU466 5.0 42.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Complex Between ADAM10 D+C Domain and A Conformation Specific Mab 8C7. within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg704 b:33.9 occ:1.00 OE2 D:GLU469 1.9 38.1 1.0 OD2 D:ASP472 2.0 39.5 1.0 OE1 D:GLU466 2.3 50.1 1.0 O D:MET464 2.4 45.2 1.0 O D:ILE459 2.4 41.3 1.0 OD1 D:ASN462 2.6 47.2 1.0 CD D:GLU469 2.9 43.4 1.0 CG D:ASP472 3.1 40.3 1.0 OE1 D:GLU469 3.4 49.2 1.0 CD D:GLU466 3.4 51.0 1.0 CG D:ASN462 3.5 48.9 1.0 C D:ILE459 3.6 41.3 1.0 C D:MET464 3.6 48.1 1.0 CB D:ASP472 3.8 39.0 1.0 ND2 D:ASN462 3.8 47.8 1.0 CA D:GLY461 4.0 39.4 1.0 N D:ASN462 4.1 36.2 1.0 OD1 D:ASP472 4.1 40.1 1.0 OE2 D:GLU466 4.2 49.9 1.0 CG D:GLU469 4.2 45.6 1.0 N D:MET464 4.2 43.4 1.0 N D:ILE459 4.2 45.9 1.0 CA D:CYS460 4.3 42.9 1.0 CG D:GLU466 4.3 47.6 1.0 N D:CYS460 4.4 44.4 1.0 CB D:GLU466 4.4 46.5 1.0 N D:GLU466 4.4 40.1 1.0 CA D:MET464 4.5 47.0 1.0 N D:VAL465 4.5 44.6 1.0 CA D:VAL465 4.5 41.1 1.0 CA D:ILE459 4.6 41.6 1.0 C D:GLY461 4.7 43.4 1.0 N D:GLY463 4.7 45.5 1.0 C D:VAL465 4.8 41.0 1.0 CB D:ASN462 4.8 47.3 1.0 CB D:MET464 4.9 48.2 1.0 CA D:ASN462 4.9 38.0 1.0

L.Atapattu, N.Saha, C.Chheang, M.F.Eissman, K.Xu, M.E.Vail, L.Hii, C.Llerena, Z.Liu, K.Horvay, H.E.Abud, U.Kusebauch, R.L.Moritz, B.S.Ding, Z.Cao, S.Rafii, M.Ernst, A.M.Scott, D.B.Nikolov, M.Lackmann, P.W.Janes. An Activated Form of ADAM10 Is Tumor Selective and Regulates Cancer Stem-Like Cells and Tumor Growth. J.Exp.Med. V. 213 1741 2016.
ISSN: ESSN 1540-9538
PubMed: 27503072
DOI: 10.1084/JEM.20151095