Magnesium in PDB 5ljw: Mamk Non-Polymerising A278D Mutant Bound to Amppnp

The structure of Mamk Non-Polymerising A278D Mutant Bound to Amppnp, PDB code: 5ljw was solved by J.Lowe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.86 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.572, 71.803, 79.761, 90.00, 98.41, 90.00
R / Rfree (%)	17.8 / 20.6

The binding sites of Magnesium atom in the Mamk Non-Polymerising A278D Mutant Bound to Amppnp (pdb code 5ljw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Mamk Non-Polymerising A278D Mutant Bound to Amppnp, PDB code: 5ljw:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Mamk Non-Polymerising A278D Mutant Bound to Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Mamk Non-Polymerising A278D Mutant Bound to Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:23.9 occ:1.00 O1B A:ANP402 1.9 25.2 1.0 O A:HOH576 2.0 25.5 1.0 O A:HOH607 2.1 24.3 1.0 O A:HOH554 2.1 26.5 1.0 O A:HOH558 2.1 24.0 1.0 O A:HOH538 2.2 28.6 1.0 PB A:ANP402 3.5 24.9 1.0 O3G A:ANP402 3.9 27.2 1.0 OD2 A:ASP161 3.9 26.6 1.0 O A:HOH620 3.9 31.9 1.0 O1G A:ANP402 4.0 31.4 1.0 O A:HOH636 4.0 25.7 1.0 O A:HOH654 4.0 31.2 1.0 OD2 A:ASP168 4.1 28.4 1.0 CA A:GLY163 4.2 20.3 1.0 O1A A:ANP402 4.2 21.8 1.0 O3A A:ANP402 4.2 26.2 1.0 O2B A:ANP402 4.3 26.0 1.0 OD1 A:ASP161 4.3 23.4 1.0 OE1 A:GLU143 4.3 27.7 1.0 PG A:ANP402 4.3 27.7 1.0 CG A:ASP161 4.4 25.4 1.0 N3B A:ANP402 4.4 28.6 1.0 N A:GLY163 4.7 20.8 1.0 O A:HOH569 4.7 25.1 1.0 O A:HOH584 4.7 31.5 1.0 PA A:ANP402 4.8 23.8 1.0 O A:HOH515 4.8 33.8 1.0 O A:THR166 4.9 19.6 1.0 OG1 A:THR166 4.9 27.0 1.0

Magnesium binding site 2 out of 2 in the Mamk Non-Polymerising A278D Mutant Bound to Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Mamk Non-Polymerising A278D Mutant Bound to Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:56.2 occ:1.00 O1B B:ANP402 1.8 51.4 1.0 O B:HOH716 2.7 49.3 1.0 PB B:ANP402 3.2 40.7 1.0 O B:HOH691 3.4 35.7 1.0 O B:HOH664 3.5 31.9 1.0 N3B B:ANP402 3.7 62.8 1.0 O B:HOH602 3.9 51.4 1.0 O2B B:ANP402 3.9 59.3 1.0 O B:HOH618 4.0 54.8 1.0 O2G B:ANP402 4.1 59.7 1.0 O B:HOH541 4.1 38.8 1.0 O B:HOH622 4.2 52.6 1.0 O3A B:ANP402 4.4 32.0 1.0 O B:HOH557 4.4 33.7 1.0 PG B:ANP402 4.6 76.3 1.0 O2A B:ANP402 4.7 36.6 1.0 PA B:ANP402 4.8 29.3 1.0 O1A B:ANP402 4.8 30.1 1.0 O B:HOH719 4.9 52.0 1.0

J.Lowe, S.He, S.H.Scheres, C.G.Savva. X-Ray and Cryo-Em Structures of Monomeric and Filamentous Actin-Like Protein Mamk Reveal Changes Associated with Polymerization. Proc. Natl. Acad. Sci. V. 113 13396 2016U.S.A..
ISSN: ESSN 1091-6490
PubMed: 27821762
DOI: 10.1073/PNAS.1612034113