Magnesium in PDB 5lse: Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A)

The structure of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A), PDB code: 5lse was solved by P.K.Fyfe, M.R.Jones, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.90 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	138.993, 138.993, 184.711, 90.00, 90.00, 120.00
R / Rfree (%)	16.8 / 20.1

The structure of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) (pdb code 5lse). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A), PDB code: 5lse:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg301 b:41.3 occ:1.00 MG L:BCL301 0.0 41.3 1.0 ND L:BCL301 2.0 42.6 1.0 NC L:BCL301 2.1 44.2 1.0 NB L:BCL301 2.1 40.8 1.0 NA L:BCL301 2.2 41.4 1.0 NE2 L:HIS173 2.3 30.3 1.0 C4D L:BCL301 3.0 43.1 1.0 C1C L:BCL301 3.0 43.3 1.0 C4B L:BCL301 3.1 42.0 1.0 C1B L:BCL301 3.1 40.5 1.0 C4A L:BCL301 3.1 39.9 1.0 C1D L:BCL301 3.1 42.2 1.0 C4C L:BCL301 3.1 40.0 1.0 C1A L:BCL301 3.2 40.7 1.0 CE1 L:HIS173 3.3 30.5 1.0 CD2 L:HIS173 3.4 30.8 1.0 CHC L:BCL301 3.4 43.4 1.0 CHB L:BCL301 3.4 39.4 1.0 CHA L:BCL301 3.5 40.8 1.0 CHD L:BCL301 3.5 41.0 1.0 CBB M:BCL402 3.6 41.8 1.0 CAB M:BCL402 3.7 43.9 1.0 OBB M:BCL402 3.7 43.1 1.0 C3D L:BCL301 4.2 42.0 1.0 C2D L:BCL301 4.3 42.6 1.0 C3B L:BCL301 4.3 40.9 1.0 C2B L:BCL301 4.3 40.5 1.0 C2C L:BCL301 4.4 40.1 1.0 C3C L:BCL301 4.4 39.2 1.0 ND1 L:HIS173 4.4 31.2 1.0 C3A L:BCL301 4.4 41.1 1.0 C3B M:BCL402 4.5 42.5 1.0 CG L:HIS173 4.5 32.4 1.0 C2A L:BCL301 4.5 39.6 1.0 CD2 L:PHE167 4.7 38.5 1.0 CBD L:BCL301 4.9 39.1 1.0 CMA L:BCL301 5.0 37.6 1.0

Magnesium binding site 2 out of 4 in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg302 b:32.9 occ:1.00 MG L:BCL302 0.0 32.9 1.0 ND L:BCL302 2.0 28.9 1.0 NC L:BCL302 2.0 29.4 1.0 NB L:BCL302 2.1 30.9 1.0 NA L:BCL302 2.1 30.1 1.0 NE2 L:HIS153 2.4 38.8 1.0 C4D L:BCL302 2.9 30.6 1.0 C1C L:BCL302 3.0 32.1 1.0 C4C L:BCL302 3.0 31.3 1.0 C1D L:BCL302 3.0 30.4 1.0 C4A L:BCL302 3.1 31.2 1.0 C4B L:BCL302 3.1 32.9 1.0 C1B L:BCL302 3.1 31.0 1.0 C1A L:BCL302 3.1 32.3 1.0 CD2 L:HIS153 3.2 39.7 1.0 CHD L:BCL302 3.4 31.8 1.0 CHC L:BCL302 3.4 32.9 1.0 CE1 L:HIS153 3.4 41.6 1.0 CHB L:BCL302 3.4 30.8 1.0 CHA L:BCL302 3.4 32.7 1.0 C3D L:BCL302 4.2 30.1 1.0 C2D L:BCL302 4.2 31.4 1.0 C2B L:BCL302 4.3 32.6 1.0 C3B L:BCL302 4.3 34.4 1.0 C3C L:BCL302 4.3 33.3 1.0 C2C L:BCL302 4.3 34.4 1.0 C3A L:BCL302 4.4 31.5 1.0 C2A L:BCL302 4.4 33.1 1.0 CG L:HIS153 4.4 41.2 1.0 ND1 L:HIS153 4.5 42.3 1.0 OBB L:BPH303 4.5 48.9 1.0 CE2 M:TYR210 4.6 39.9 1.0 CBA L:BCL302 4.9 35.6 1.0 CBD L:BCL302 4.9 34.6 1.0 CD2 L:LEU154 4.9 41.2 1.0 C19 L:BPH303 4.9 37.9 1.0 CBC L:BCL302 4.9 33.6 1.0

Magnesium binding site 3 out of 4 in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg401 b:34.6 occ:1.00 MG M:BCL401 0.0 34.6 1.0 ND M:BCL401 2.0 33.2 1.0 NC M:BCL401 2.0 33.9 1.0 NB M:BCL401 2.1 34.0 1.0 NA M:BCL401 2.2 33.9 1.0 NE2 M:HIS182 2.3 41.0 1.0 C4D M:BCL401 3.0 33.0 1.0 C1C M:BCL401 3.0 34.4 1.0 C4C M:BCL401 3.0 34.5 1.0 C4B M:BCL401 3.0 33.8 1.0 C1B M:BCL401 3.1 35.2 1.0 C1D M:BCL401 3.1 32.9 1.0 C4A M:BCL401 3.1 35.0 1.0 CD2 M:HIS182 3.2 42.6 1.0 C1A M:BCL401 3.2 36.8 1.0 CHC M:BCL401 3.4 34.7 1.0 CE1 M:HIS182 3.4 41.7 1.0 CHB M:BCL401 3.4 34.1 1.0 CHD M:BCL401 3.4 32.6 1.0 CHA M:BCL401 3.5 33.8 1.0 C3D M:BCL401 4.2 32.7 1.0 C2D M:BCL401 4.2 32.8 1.0 C2B M:BCL401 4.3 35.9 1.0 C3B M:BCL401 4.3 34.8 1.0 C2C M:BCL401 4.3 34.9 1.0 C3C M:BCL401 4.3 34.4 1.0 CG M:HIS182 4.4 41.5 1.0 C3A M:BCL401 4.5 37.3 1.0 ND1 M:HIS182 4.5 43.4 1.0 C2A M:BCL401 4.6 38.3 1.0 OBB M:BPH403 4.8 51.3 1.0 CE2 L:PHE181 4.8 38.9 1.0 CBD M:BCL401 4.9 35.5 1.0 CM5 M:SPN405 5.0 42.9 1.0

Magnesium binding site 4 out of 4 in the Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Photosynthetic Reaction Center Mutant with Glu L212 Replaced with Ala (Chain L, EL212W), Asp L213 Replaced with Ala (Chain L, DL213A) and Leu M215 Replaced with Ala (Chain M, LM215A) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg402 b:39.4 occ:1.00 MG M:BCL402 0.0 39.4 1.0 ND M:BCL402 2.0 38.6 1.0 NC M:BCL402 2.1 42.1 1.0 NB M:BCL402 2.1 41.8 1.0 NA M:BCL402 2.2 39.3 1.0 NE2 M:HIS202 2.4 31.9 1.0 C4D M:BCL402 3.0 39.5 1.0 C1C M:BCL402 3.0 40.1 1.0 C4B M:BCL402 3.1 41.8 1.0 C1B M:BCL402 3.1 42.6 1.0 C1D M:BCL402 3.1 40.2 1.0 C4C M:BCL402 3.1 38.9 1.0 C4A M:BCL402 3.2 39.7 1.0 CD2 M:HIS202 3.3 33.0 1.0 C1A M:BCL402 3.3 38.5 1.0 CHC M:BCL402 3.4 41.6 1.0 CE1 M:HIS202 3.4 32.8 1.0 CHD M:BCL402 3.5 39.5 1.0 CHB M:BCL402 3.5 39.0 1.0 CHA M:BCL402 3.5 38.9 1.0 CBB L:BCL301 3.5 42.6 1.0 CAB L:BCL301 3.7 40.9 1.0 OBB L:BCL301 3.8 38.9 1.0 C3D M:BCL402 4.2 39.1 1.0 C2D M:BCL402 4.3 39.8 1.0 C2B M:BCL402 4.3 42.5 1.0 C3B M:BCL402 4.3 42.5 1.0 C2C M:BCL402 4.3 40.7 1.0 C3C M:BCL402 4.4 39.4 1.0 CG M:HIS202 4.4 33.3 1.0 C3B L:BCL301 4.4 40.9 1.0 ND1 M:HIS202 4.5 32.7 1.0 C3A M:BCL402 4.5 40.1 1.0 C2A M:BCL402 4.6 40.6 1.0 CBD M:BCL402 4.9 39.1 1.0

V.M.Friebe, D.J.Swainsbury, P.K.Fyfe, W.Van Der Heijden, M.R.Jones, R.N.Frese. On the Mechanism of Ubiquinone Mediated Photocurrent Generation By A Reaction Center Based Photocathode. Biochim.Biophys.Acta V.1857 1925 2016.
ISSN: ISSN 0006-3002
PubMed: 27687473
DOI: 10.1016/J.BBABIO.2016.09.011