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Magnesium in PDB 5mb9: Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair

Protein crystallography data

The structure of Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair, PDB code: 5mb9 was solved by A.Gumiero, F.A.Weyer, G.Valentin Gese, K.Lapouge, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.97 / 3.20
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 93.918, 179.359, 155.455, 90.00, 90.00, 90.00
R / Rfree (%) 22.7 / 28.5

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair (pdb code 5mb9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair, PDB code: 5mb9:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 5mb9

Go back to Magnesium Binding Sites List in 5mb9
Magnesium binding site 1 out of 2 in the Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg701

b:70.5
occ:1.00
O A:HOH802 2.0 87.8 1.0
O A:HOH804 2.0 79.2 1.0
O2B A:ATP700 2.2 70.4 1.0
O A:HOH801 2.6 82.0 1.0
O A:HOH803 2.6 54.6 1.0
O2G A:ATP700 3.1 88.5 1.0
OD2 A:ASP211 3.3 0.0 1.0
PB A:ATP700 3.7 67.9 1.0
OD1 A:ASP211 3.9 0.6 1.0
NE2 A:GLN390 4.0 98.1 1.0
CG A:ASP211 4.0 0.0 1.0
OE2 A:GLU186 4.0 0.9 1.0
O2A A:ATP700 4.1 69.8 1.0
PG A:ATP700 4.2 80.3 1.0
OE1 A:GLU186 4.2 0.7 1.0
CD A:GLU186 4.3 0.7 1.0
O3A A:ATP700 4.4 66.4 1.0
OE1 A:GLN390 4.4 0.2 1.0
O3B A:ATP700 4.4 73.5 1.0
O1G A:ATP700 4.5 94.6 1.0
NH2 A:ARG80 4.6 0.6 1.0
CD A:GLN390 4.7 97.2 1.0
O1B A:ATP700 4.8 72.6 1.0
PA A:ATP700 4.9 62.0 1.0

Magnesium binding site 2 out of 2 in 5mb9

Go back to Magnesium Binding Sites List in 5mb9
Magnesium binding site 2 out of 2 in the Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Eukaryotic Ribosome Associated Complex (Rac), A Unique HSP70/HSP40 Pair within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg602

b:67.7
occ:1.00
O B:HOH701 2.1 75.9 1.0
O1B B:ATP601 2.2 61.6 1.0
O B:HOH703 2.4 0.1 1.0
O B:HOH704 2.6 64.1 1.0
O B:HOH702 2.7 52.1 1.0
NE2 B:GLN390 2.9 93.1 1.0
OE1 B:GLN390 3.3 77.1 1.0
OD1 B:ASP211 3.4 94.2 1.0
CD B:GLN390 3.5 85.5 1.0
OD2 B:ASP211 3.6 96.9 1.0
O2G B:ATP601 3.7 61.9 1.0
PB B:ATP601 3.7 80.2 1.0
CG B:ASP211 3.9 90.1 1.0
OE2 B:GLU186 4.0 69.7 1.0
O1A B:ATP601 4.1 53.2 1.0
CD B:GLU186 4.2 71.0 1.0
OE1 B:GLU186 4.3 74.5 1.0
O3A B:ATP601 4.5 63.1 1.0
O2B B:ATP601 4.6 61.0 1.0
PG B:ATP601 4.7 74.8 1.0
O3B B:ATP601 4.7 72.6 1.0
CG B:GLN390 5.0 83.8 1.0
PA B:ATP601 5.0 54.3 1.0
O1G B:ATP601 5.0 91.1 1.0

Reference:

F.A.Weyer, A.Gumiero, G.V.Gese, K.Lapouge, I.Sinning. Structural Insights Into A Unique HSP70-HSP40 Interaction in the Eukaryotic Ribosome-Associated Complex. Nat. Struct. Mol. Biol. V. 24 144 2017.
ISSN: ESSN 1545-9985
PubMed: 28067917
DOI: 10.1038/NSMB.3349
Page generated: Mon Dec 14 20:51:02 2020

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