Magnesium in PDB 5tij: Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer)

All present enzymatic activity of Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer):
4.2.1.11;

The structure of Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer), PDB code: 5tij was solved by P.G.Leonard, F.L.Muller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.01 / 2.63
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	68.250, 108.380, 117.480, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 26.7

The binding sites of Magnesium atom in the Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer) (pdb code 5tij). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer), PDB code: 5tij:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:31.4 occ:1.00 OE2 A:GLU293 2.1 29.0 1.0 O11 A:5TX501 2.1 26.9 1.0 O A:HOH622 2.1 31.2 1.0 OD1 A:ASP245 2.1 24.6 1.0 OD1 A:ASP318 2.1 22.6 1.0 O10 A:5TX501 2.3 30.3 1.0 CG A:ASP245 2.7 23.3 1.0 OD2 A:ASP245 2.8 34.7 1.0 N12 A:5TX501 2.8 30.1 1.0 C04 A:5TX501 3.0 30.0 1.0 CG A:ASP318 3.2 28.2 1.0 CD A:GLU293 3.2 20.5 1.0 NZ A:LYS394 3.7 13.7 1.0 CB A:ASP318 3.8 36.1 1.0 OE1 A:GLN166 4.0 14.3 1.0 OE1 A:GLU293 4.0 31.3 1.0 CB A:ASP245 4.1 20.9 1.0 CG A:GLU293 4.2 13.3 1.0 NZ A:LYS343 4.2 15.1 1.0 C01 A:5TX501 4.2 23.5 1.0 OD2 A:ASP318 4.2 21.0 1.0 CD2 A:LEU341 4.3 13.6 1.0 C03 A:5TX501 4.4 31.8 1.0 O13 A:5TX501 4.4 33.5 1.0 OD2 A:ASP294 4.5 22.8 1.0 OE2 A:GLU167 4.7 12.9 1.0 CE A:LYS394 4.8 12.9 1.0 C02 A:5TX501 4.9 18.5 1.0

Magnesium binding site 2 out of 2 in the Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Human Enolase 2 with ((3S,5S)-1,5-Dihydroxy-3-Methyl-2- Oxopyrrolidin-3-Yl)Phosphonate (Purified Enantiomer) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:36.9 occ:1.00 OE2 B:GLU293 2.1 36.3 1.0 OD1 B:ASP318 2.2 37.3 1.0 OD2 B:ASP245 2.2 34.3 1.0 O B:HOH609 2.2 24.8 1.0 O10 B:5TX501 2.3 20.0 1.0 O11 B:5TX501 2.3 36.9 1.0 CG B:ASP245 2.7 33.6 1.0 OD1 B:ASP245 2.8 35.5 1.0 N12 B:5TX501 3.0 40.5 1.0 C04 B:5TX501 3.1 33.4 1.0 CD B:GLU293 3.2 23.3 1.0 CG B:ASP318 3.3 36.3 1.0 CB B:ASP318 3.9 38.5 1.0 NZ B:LYS394 4.0 16.4 1.0 OE1 B:GLU293 4.0 25.8 1.0 CG B:GLU293 4.1 22.5 1.0 CB B:ASP245 4.1 29.2 1.0 NE2 B:GLN166 4.2 29.7 1.0 NZ B:LYS343 4.2 17.6 1.0 OD2 B:ASP318 4.2 31.7 1.0 OD2 B:ASP294 4.3 36.4 1.0 C01 B:5TX501 4.4 38.4 1.0 C03 B:5TX501 4.4 36.6 1.0 CD2 B:LEU341 4.5 17.2 1.0 OE1 B:GLU167 4.5 30.3 1.0 O13 B:5TX501 4.7 29.3 1.0 OG B:SER40 4.8 56.3 1.0 CB B:ALA247 4.8 25.9 1.0

Y.H.Lin, N.Satani, N.Hammoudi, J.J.Ackroyd, S.Khadka, Y.C.Yan, D.K.Georgiou, Y.Sun, R.Zielinski, T.Tran, S.Castro Pando, X.Wang, D.Maxwell, Z.Peng, F.Pisaneschi, P.J.Mandal, P.G.Leonard, Q.Xu, Q.Wu, Y.Jiang, B.Czako, Z.Kang, J.M.Asara, W.Priebe, W.Bornmann, J.R.Marszalek, R.A.Depinho, F.L.Muller. Eradication of ENO1-Deleted Glioblastoma Through Collateral Lethality Biorxiv 2019.
DOI: 10.1101/331538