Magnesium in PDB 5tjg: Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp

Enzymatic activity of Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp

All present enzymatic activity of Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp:
2.7.7.6;

Protein crystallography data

The structure of Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp, PDB code: 5tjg was solved by S.A.Darst, B.Bae, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 38.63 / 2.60
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 104.098, 111.423, 143.177, 104.12, 99.66, 109.43
R / Rfree (%) 22.4 / 26.2

Other elements in 5tjg:

The structure of Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp (pdb code 5tjg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp, PDB code: 5tjg:

Magnesium binding site 1 out of 1 in 5tjg

Go back to Magnesium Binding Sites List in 5tjg
Magnesium binding site 1 out of 1 in the Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermus Aquaticus DELTA1.1-Sigmaa Holoenzyme/Downstream-Fork Promoter Complex with An Open Clamp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg2003

b:75.0
occ:1.00
OD1 D:ASP741 3.2 69.7 1.0
OD1 D:ASP743 3.3 75.9 1.0
OD2 D:ASP743 3.3 72.2 1.0
CG D:ASP743 3.6 53.1 1.0
OD2 D:ASP741 3.6 82.5 1.0
CB D:ASP739 3.8 35.6 1.0
CG D:ASP741 3.8 71.0 1.0
OD2 D:ASP739 4.2 59.2 1.0
NH2 D:ARG704 4.5 67.9 1.0
CG D:ASP739 4.5 49.4 1.0
O D:ASP739 4.7 51.4 1.0
CA D:ASP739 4.9 55.4 1.0

Reference:

A.Feklistov, B.Bae, J.Hauver, A.Lass-Napiorkowska, M.Kalesse, F.Glaus, K.H.Altmann, T.Heyduk, R.Landick, S.A.Darst. Rna Polymerase Motions During Promoter Melting. Science V. 356 863 2017.
ISSN: ESSN 1095-9203
PubMed: 28546214
DOI: 10.1126/SCIENCE.AAM7858
Page generated: Mon Dec 14 21:12:24 2020

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