Magnesium in PDB 5tky: Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation

The structure of Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation, PDB code: 5tky was solved by A.Gumiero, G.V.Gese, F.A.Weyer, K.Lapouge, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.43 / 2.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.930, 128.240, 79.680, 90.00, 93.22, 90.00
R / Rfree (%)	22.8 / 26.4

The binding sites of Magnesium atom in the Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation (pdb code 5tky). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation, PDB code: 5tky:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg701 b:28.5 occ:1.00 O A:HOH808 2.0 33.9 1.0 O A:HOH812 2.0 30.5 1.0 O2G A:ATP700 2.0 38.0 1.0 O2B A:ATP700 2.1 35.9 1.0 O A:HOH803 2.2 37.6 1.0 O A:HOH817 2.3 34.2 1.0 PG A:ATP700 3.2 40.6 1.0 OE1 A:GLU178 3.4 38.0 1.0 PB A:ATP700 3.4 38.2 1.0 O3B A:ATP700 3.6 45.3 1.0 O1G A:ATP700 3.6 43.6 1.0 O2A A:ATP700 3.9 39.5 1.0 O A:HOH810 3.9 39.5 1.0 OD1 A:ASP13 4.1 41.7 1.0 OD2 A:ASP13 4.1 36.9 1.0 CD A:GLU178 4.1 39.1 1.0 O3A A:ATP700 4.4 35.3 1.0 OE2 A:GLU178 4.4 36.8 1.0 O1B A:ATP700 4.4 37.9 1.0 O3G A:ATP700 4.5 38.3 1.0 CG A:ASP13 4.5 39.8 1.0 CA A:GLY15 4.6 45.4 1.0 OD1 A:ASP203 4.6 47.0 1.0 NZ A:LYS74 4.6 44.0 1.0 OD2 A:ASP203 4.7 49.2 1.0 PA A:ATP700 4.7 36.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Co-Translational HSP70 Chaperone Ssb in the Atp-Bound, Open Conformation within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg701 b:33.7 occ:1.00 O1B B:ATP700 2.0 39.7 1.0 O B:HOH810 2.0 35.3 1.0 O B:HOH814 2.0 31.9 1.0 O B:HOH804 2.1 44.8 1.0 O B:HOH809 2.3 44.2 1.0 O2G B:ATP700 2.4 42.5 1.0 PB B:ATP700 3.4 47.5 1.0 OE1 B:GLU178 3.4 51.2 1.0 PG B:ATP700 3.5 40.2 1.0 O1A B:ATP700 3.8 48.1 1.0 O3B B:ATP700 3.8 43.0 1.0 O3G B:ATP700 3.9 41.5 1.0 O B:HOH824 4.2 40.3 1.0 CD B:GLU178 4.2 47.2 1.0 OD1 B:ASP13 4.2 51.9 1.0 OD2 B:ASP13 4.2 42.2 1.0 O3A B:ATP700 4.3 35.4 1.0 OD1 B:ASP203 4.3 38.4 1.0 OD2 B:ASP203 4.4 49.0 1.0 O2B B:ATP700 4.5 40.2 1.0 PA B:ATP700 4.5 34.9 1.0 OE2 B:GLU178 4.6 46.5 1.0 CG2 B:VAL373 4.6 29.0 1.0 CG B:ASP13 4.6 41.9 1.0 CG B:ASP203 4.8 50.3 1.0 O1G B:ATP700 4.8 40.0 1.0 CA B:GLY15 4.9 32.8 1.0 CA B:GLY205 5.0 38.4 1.0

A.Gumiero, C.Conz, G.V.Gese, Y.Zhang, F.A.Weyer, K.Lapouge, J.Kappes, U.Von Plehwe, G.Schermann, E.Fitzke, T.Wolfle, T.Fischer, S.Rospert, I.Sinning. Interaction of the Cotranslational HSP70 Ssb with Ribosomal Proteins and Rrna Depends on Its Lid Domain. Nat Commun V. 7 13563 2016.
ISSN: ESSN 2041-1723
PubMed: 27882919
DOI: 10.1038/NCOMMS13563