Magnesium in PDB 5tyb: Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min)

All present enzymatic activity of Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min):
2.7.7.7;

The structure of Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min), PDB code: 5tyb was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.39 / 1.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	60.047, 68.488, 110.450, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 19.9

The structure of Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min) also contains other interesting chemical elements:

Calcium	(Ca)	1 atom
Sodium	(Na)	1 atom

The binding sites of Magnesium atom in the Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min) (pdb code 5tyb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min), PDB code: 5tyb:

Magnesium binding site 1 out of 1 in the Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dna Polymerase Mu Reactant Complex, 10MM MG2+ (7.5 Min) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:12.8 occ:1.00 OD1 A:ASP330 2.0 18.7 1.0 O32 A:PPV508 2.0 17.9 0.1 O A:HOH647 2.1 18.1 1.0 O1B A:TTP502 2.1 15.1 0.7 O1G A:TTP502 2.1 17.7 0.7 O1A A:TTP502 2.1 12.7 0.7 OD2 A:ASP332 2.1 12.8 1.0 O21 A:PPV508 2.2 15.1 0.3 OP1 P:DT5 2.5 14.2 0.3 CG A:ASP330 3.1 20.8 1.0 PB A:TTP502 3.2 17.4 0.7 CG A:ASP332 3.2 14.6 1.0 P1 A:PPV508 3.2 17.2 0.3 PG A:TTP502 3.3 22.7 0.7 P2 A:PPV508 3.3 22.4 0.1 PA A:TTP502 3.3 13.8 0.7 O31 A:PPV508 3.3 18.2 0.3 O3A A:TTP502 3.5 18.3 0.7 OD2 A:ASP330 3.6 25.9 1.0 OD1 A:ASP332 3.6 11.8 1.0 CA A:CA504 3.6 18.2 1.0 O3B A:TTP502 3.7 21.4 0.7 OPP A:PPV508 3.7 20.7 0.1 O A:ASP330 3.8 14.7 1.0 P P:DT5 3.9 17.7 0.3 O2G A:TTP502 4.0 22.6 0.7 O22 A:PPV508 4.1 22.1 0.1 C A:ASP330 4.2 14.7 1.0 O A:HOH623 4.2 19.4 1.0 C5' A:TTP502 4.2 13.8 0.7 O A:HOH620 4.3 14.2 1.0 O5' A:TTP502 4.3 15.9 0.7 ND1 A:HIS329 4.3 26.5 0.7 N A:GLY320 4.3 10.1 1.0 O12 A:PPV508 4.4 22.9 0.1 CB A:ASP330 4.4 14.5 1.0 O2A A:TTP502 4.4 17.7 0.7 O5' P:DT5 4.4 15.8 0.3 C5' P:DT5 4.4 14.2 0.3 CE1 A:HIS329 4.4 24.4 0.7 O3G A:TTP502 4.5 23.2 0.7 O2B A:TTP502 4.5 17.7 0.7 CB A:ASP332 4.5 10.2 1.0 O11 A:PPV508 4.6 17.6 0.3 N A:ASP330 4.6 15.4 1.0 CA A:ASP330 4.6 17.1 1.0 CA A:GLY319 4.6 10.2 1.0 N A:VAL331 4.8 11.5 1.0 N A:ASP332 4.8 10.7 1.0 O3' P:DA4 4.8 19.3 0.3 OP2 P:DT5 4.9 18.7 0.3 O P:HOH202 5.0 24.5 1.0

J.A.Jamsen, W.A.Beard, L.C.Pedersen, D.D.Shock, A.F.Moon, J.M.Krahn, K.Bebenek, T.A.Kunkel, S.H.Wilson. Time-Lapse Crystallography Snapshots of A Double-Strand Break Repair Polymerase in Action. Nat Commun V. 8 253 2017.
ISSN: ESSN 2041-1723
PubMed: 28811466
DOI: 10.1038/S41467-017-00271-7