Magnesium in PDB 5uac: Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type

All present enzymatic activity of Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type:
2.7.7.6;

The structure of Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type, PDB code: 5uac was solved by V.Molodtsov, N.T.Scharf, M.A.Stefan, G.A.Garcia, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.98 / 3.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	188.958, 204.430, 313.619, 90.00, 90.00, 90.00
R / Rfree (%)	24.8 / 29.6

The structure of Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type (pdb code 5uac). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type, PDB code: 5uac:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1501 b:0.4 occ:1.00 OD2 D:ASP462 1.8 0.4 1.0 OD1 D:ASP462 2.0 1.0 1.0 CG D:ASP462 2.1 0.4 1.0 OD2 D:ASP464 2.4 0.0 1.0 OD1 D:ASP460 2.5 0.7 1.0 CB D:ASP460 2.8 0.0 1.0 OD1 D:ASP464 2.9 0.4 1.0 CG D:ASP464 3.0 0.3 1.0 CG D:ASP460 3.1 0.7 1.0 CB D:ASP462 3.6 0.9 1.0 CA D:ASP460 4.0 0.6 1.0 N D:ASP462 4.1 0.8 1.0 N D:ASP460 4.3 0.2 1.0 CA D:ASP462 4.3 0.4 1.0 OD2 D:ASP460 4.3 0.5 1.0 C D:ASP460 4.4 0.6 1.0 CB D:ASP464 4.6 0.8 1.0 C D:ASP462 4.7 0.8 1.0 N D:ASP464 4.8 100.0 1.0 O D:ASP462 4.8 0.5 1.0 O D:ASP460 4.9 99.8 1.0 N D:PHE461 4.9 0.6 1.0

Magnesium binding site 2 out of 2 in the Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Escherichia Coli Rna Polymerase and Rifampin Complex, Wild-Type within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1501 b:0.6 occ:1.00 OD2 J:ASP462 1.8 0.6 1.0 OD2 J:ASP464 1.9 0.3 1.0 OD1 J:ASP462 2.1 0.5 1.0 CG J:ASP462 2.2 0.5 1.0 CB J:ASP460 2.6 0.9 1.0 OD1 J:ASP460 2.7 0.1 1.0 CG J:ASP464 2.7 0.8 1.0 OD1 J:ASP464 2.8 1.0 1.0 CG J:ASP460 3.1 0.6 1.0 CA J:ASP460 3.7 0.5 1.0 CB J:ASP462 3.7 0.0 1.0 N J:ASP460 3.9 0.2 1.0 N J:ASP462 4.0 0.7 1.0 C J:ASP460 4.0 0.2 1.0 CB J:ASP464 4.3 0.0 1.0 CA J:ASP462 4.3 0.6 1.0 OD2 J:ASP460 4.4 0.3 1.0 O J:ASP460 4.5 0.3 1.0 N J:PHE461 4.6 0.3 1.0 N J:ASP464 4.7 0.2 1.0 C J:ASP462 4.7 0.4 1.0 NH1 J:ARG425 4.9 0.6 1.0

V.Molodtsov, N.T.Scharf, M.A.Stefan, G.A.Garcia, K.S.Murakami. Structural Basis For Rifamycin Resistance of Bacterial Rna Polymerase By the Three Most Clinically Important Rpob Mutations Found in Mycobacterium Tuberculosis. Mol. Microbiol. V. 103 1034 2017.
ISSN: ESSN 1365-2958
PubMed: 28009073
DOI: 10.1111/MMI.13606