Magnesium in PDB 5ucm: Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa

All present enzymatic activity of Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa:
6.1.1.15;

The structure of Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa, PDB code: 5ucm was solved by Seattle Structural Genomics Center For Infectious Disease, Seattlestructural Genomics Center For Infectious Disease (Ssgcid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.00 / 2.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	85.830, 101.540, 187.240, 90.00, 90.00, 90.00
R / Rfree (%)	16.9 / 23.2

The binding sites of Magnesium atom in the Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa (pdb code 5ucm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa, PDB code: 5ucm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg600 b:45.0 occ:1.00 O A:HOH841 2.0 39.1 1.0 O A:HOH915 2.1 31.6 1.0 O A:HOH843 2.3 28.9 1.0 OE2 A:GLU209 3.1 32.4 1.0 OE1 A:GLU408 3.5 39.2 1.0 OE1 A:GLU234 3.6 32.9 1.0 O A:HOH714 3.7 39.5 1.0 OD1 A:ASP219 3.8 37.6 1.0 CD A:GLU209 4.1 28.8 1.0 OE2 A:GLU234 4.1 33.7 1.0 ND2 A:ASN232 4.2 28.0 1.0 CD A:GLU234 4.2 34.6 1.0 O A:HOH775 4.3 27.0 1.0 OE1 A:GLU209 4.5 30.1 1.0 CG A:ASP219 4.6 38.3 1.0 OD2 A:ASP219 4.7 40.6 1.0 CD A:GLU408 4.7 32.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg600 b:52.2 occ:1.00 O B:HOH930 2.1 53.0 1.0 O B:HOH872 2.1 48.4 1.0 O B:HOH922 2.2 51.2 1.0 O B:HOH830 2.2 37.9 1.0 OE1 B:GLU408 3.1 33.0 1.0 OE1 B:GLU209 3.5 33.1 1.0 OD1 B:ASP219 3.8 37.4 1.0 CD B:GLU408 4.1 28.3 1.0 CD B:GLU209 4.3 26.3 1.0 OE1 B:GLU234 4.4 38.1 1.0 OE2 B:GLU408 4.5 33.7 1.0 ND2 B:ASN232 4.6 29.0 1.0 OE2 B:GLU209 4.6 23.9 1.0 OE2 B:GLU234 4.7 34.4 1.0 CG B:ASP219 4.8 34.7 1.0 CD B:GLU234 4.9 31.6 1.0

N.Pena, D.M.Dranow, Y.Hu, Y.Escamilla, J.M.Bullard. Characterization and Structure Determination of Prolyl-Trna Synthetase From Pseudomonas Aeruginosa and Development As A Screening Platform. Protein Sci. 2019.
ISSN: ESSN 1469-896X
PubMed: 30666738
DOI: 10.1002/PRO.3579