Magnesium in PDB 5usa: 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant

All present enzymatic activity of 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant:
3.1.26.4;

The structure of 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant, PDB code: 5usa was solved by Z.Fang, L.Qin, Z.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.66 / 1.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	81.201, 37.731, 62.238, 90.00, 96.38, 90.00
R / Rfree (%)	19 / 21.6

The binding sites of Magnesium atom in the 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant (pdb code 5usa). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant, PDB code: 5usa:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:12.9 occ:1.00 OD1 A:ASP71 2.1 18.7 1.0 OD1 A:ASN132 2.2 21.0 1.0 OE2 A:GLU109 2.3 18.3 1.0 O3' B:A6 2.4 18.9 1.0 ND2 A:ASN132 2.5 20.0 1.0 CG A:ASN132 2.7 19.3 1.0 MG A:MG201 2.9 48.0 1.0 O A:HOH309 3.1 16.5 1.0 CG A:ASP71 3.1 18.5 1.0 CD A:GLU109 3.2 16.1 1.0 OE1 A:GLU109 3.4 15.6 1.0 OD2 A:ASP71 3.6 19.4 1.0 C3' B:A6 3.7 18.4 1.0 O2' B:A6 4.0 16.6 1.0 C4' B:A6 4.0 19.1 1.0 CB A:ASN132 4.1 17.5 1.0 CB A:ASP71 4.4 16.7 1.0 C2' B:A6 4.4 18.5 1.0 CG A:GLU109 4.6 14.5 1.0 C5' B:A6 4.7 18.3 1.0 CG1 A:VAL72 4.7 18.4 1.0 CA A:ASP71 4.7 17.2 1.0 N A:VAL72 4.8 16.9 1.0 CG2 A:ILE189 4.8 18.1 1.0 CA A:ASN132 4.8 16.6 1.0 N A:ASN132 4.8 14.2 1.0 C A:ASN132 4.9 15.7 1.0

Magnesium binding site 2 out of 2 in the 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 5-Se-T2-Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:48.0 occ:1.00 OD2 A:ASP71 2.3 19.4 1.0 OD1 A:ASP192 2.8 38.4 1.0 MG B:MG201 2.9 12.9 1.0 OD1 A:ASP71 3.0 18.7 1.0 CG A:ASP71 3.0 18.5 1.0 O3' B:A6 3.3 18.9 1.0 ND2 A:ASN132 3.4 20.0 1.0 OE1 A:GLU188 3.8 62.4 1.0 CG A:ASP192 3.9 35.3 1.0 CG2 A:ILE189 4.4 18.1 1.0 CG A:ASN132 4.4 19.3 1.0 CB A:ASP71 4.4 16.7 1.0 CB A:ASP192 4.5 31.1 1.0 O A:HOH306 4.5 20.9 1.0 C3' B:A6 4.6 18.4 1.0 O A:VAL72 4.6 15.5 1.0 OD1 A:ASN132 4.6 21.0 1.0 CA A:ASP192 4.7 26.8 1.0 OE2 A:GLU109 4.8 18.3 1.0 OD2 A:ASP192 4.9 40.3 1.0 CD A:GLU188 5.0 51.2 1.0

Z.Fang, L.Qin, Z.Huang. 5-Se-T Dna and Native Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant To Be Published.