Magnesium in PDB 5v33: R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip

The structure of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip, PDB code: 5v33 was solved by J.M.Schieferstein, A.S.Pawate, C.Sun, F.Wan, J.Broecker, O.P.Ernst, R.B.Gennis, P.J.A.Kenis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.08 / 3.49
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	102.332, 102.332, 240.333, 90.00, 90.00, 90.00
R / Rfree (%)	22.1 / 26.2

The structure of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip (pdb code 5v33). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip, PDB code: 5v33:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg302 b:31.4 occ:1.00 MG L:BCL302 0.0 31.4 1.0 ND L:BCL302 2.0 31.2 1.0 NB L:BCL302 2.1 31.3 1.0 NC L:BCL302 2.1 31.7 1.0 NA L:BCL302 2.2 30.9 1.0 NE2 L:HIS153 2.9 33.5 1.0 C4D L:BCL302 3.0 32.9 1.0 C4B L:BCL302 3.1 31.5 1.0 C1B L:BCL302 3.1 31.4 1.0 C1C L:BCL302 3.1 32.0 1.0 C4A L:BCL302 3.1 30.8 1.0 C1D L:BCL302 3.1 31.6 1.0 C4C L:BCL302 3.2 32.0 1.0 C1A L:BCL302 3.2 32.6 1.0 CHC L:BCL302 3.4 31.9 1.0 CHB L:BCL302 3.4 30.9 1.0 CHA L:BCL302 3.5 32.7 1.0 CHD L:BCL302 3.5 32.2 1.0 CD2 L:HIS153 3.6 34.1 1.0 CE1 L:HIS153 3.8 34.0 1.0 C3D L:BCL302 4.2 33.1 1.0 C2D L:BCL302 4.3 31.7 1.0 C2B L:BCL302 4.3 31.4 1.0 C3B L:BCL302 4.3 31.4 1.0 OBB L:BPH301 4.4 29.2 1.0 C2C L:BCL302 4.4 32.6 1.0 C3A L:BCL302 4.5 30.8 1.0 C3C L:BCL302 4.5 32.4 1.0 C2A L:BCL302 4.5 30.5 1.0 CG L:HIS153 4.7 43.5 1.0 ND1 L:HIS153 4.8 42.7 1.0 CBD L:BCL302 4.9 31.1 1.0

Magnesium binding site 2 out of 4 in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg303 b:50.1 occ:1.00 MG L:BCL303 0.0 50.1 1.0 ND L:BCL303 2.0 47.5 1.0 NB L:BCL303 2.1 45.2 1.0 NC L:BCL303 2.1 46.9 1.0 NA L:BCL303 2.2 47.5 1.0 CE1 L:HIS173 2.9 38.7 1.0 NE2 L:HIS173 3.0 38.8 1.0 C4D L:BCL303 3.0 48.3 1.0 C4B L:BCL303 3.1 45.5 1.0 C1B L:BCL303 3.1 44.1 1.0 C1C L:BCL303 3.1 47.1 1.0 C4A L:BCL303 3.1 45.7 1.0 C1D L:BCL303 3.1 48.0 1.0 C4C L:BCL303 3.2 47.4 1.0 C1A L:BCL303 3.2 47.5 1.0 CHC L:BCL303 3.4 47.2 1.0 CHB L:BCL303 3.4 44.4 1.0 CHA L:BCL303 3.5 48.2 1.0 CHD L:BCL303 3.5 47.7 1.0 OBB M:BCL402 3.5 34.0 1.0 CAB M:BCL402 3.6 33.2 1.0 CBB M:BCL402 3.7 32.5 1.0 C3D L:BCL303 4.2 49.7 1.0 ND1 L:HIS173 4.2 39.9 1.0 C2B L:BCL303 4.3 42.5 1.0 C2D L:BCL303 4.3 50.0 1.0 C3B L:BCL303 4.3 43.8 1.0 CD2 L:HIS173 4.3 40.2 1.0 C3B M:BCL402 4.3 33.3 1.0 C3A L:BCL303 4.4 44.1 1.0 C2C L:BCL303 4.4 47.0 1.0 C3C L:BCL303 4.5 47.8 1.0 C2A L:BCL303 4.5 45.0 1.0 CD2 L:PHE167 4.9 37.5 1.0 CMA L:BCL303 4.9 44.8 1.0 CBD L:BCL303 4.9 48.2 1.0 CG L:HIS173 5.0 41.4 1.0 C4B M:BCL402 5.0 34.3 1.0

Magnesium binding site 3 out of 4 in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg304 b:34.4 occ:1.00 MG L:BCL304 0.0 34.4 1.0 ND L:BCL304 2.0 36.6 1.0 NB L:BCL304 2.1 36.8 1.0 NC L:BCL304 2.1 36.5 1.0 NA L:BCL304 2.2 36.7 1.0 C4D L:BCL304 3.0 36.5 1.0 C1B L:BCL304 3.1 37.0 1.0 C4B L:BCL304 3.1 36.8 1.0 C1C L:BCL304 3.1 36.5 1.0 C1D L:BCL304 3.1 36.5 1.0 C4A L:BCL304 3.1 36.9 1.0 C4C L:BCL304 3.2 36.5 1.0 C1A L:BCL304 3.2 36.5 1.0 CE1 M:HIS182 3.4 47.2 1.0 CHB L:BCL304 3.4 37.1 1.0 CHC L:BCL304 3.4 36.6 1.0 CHA L:BCL304 3.5 36.7 1.0 CHD L:BCL304 3.5 36.6 1.0 NE2 M:HIS182 3.9 48.0 1.0 ND1 M:HIS182 4.1 47.7 1.0 C3D L:BCL304 4.2 36.5 1.0 C2B L:BCL304 4.3 37.1 1.0 C2D L:BCL304 4.3 36.4 1.0 C3B L:BCL304 4.3 37.1 1.0 C2C L:BCL304 4.4 36.6 1.0 C3A L:BCL304 4.4 37.0 1.0 C3C L:BCL304 4.5 36.4 1.0 C2A L:BCL304 4.5 36.4 1.0 CBB M:BPH403 4.6 40.1 1.0 CD2 M:HIS182 4.9 49.0 1.0 CBD L:BCL304 4.9 37.1 1.0

Magnesium binding site 4 out of 4 in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg402 b:66.2 occ:1.00 MG M:BCL402 0.0 66.2 1.0 ND M:BCL402 2.0 35.5 1.0 NB M:BCL402 2.1 34.0 1.0 NC M:BCL402 2.1 36.3 1.0 NA M:BCL402 2.2 34.0 1.0 CE1 M:HIS202 2.9 34.3 1.0 C4D M:BCL402 3.0 35.3 1.0 C4B M:BCL402 3.1 34.3 1.0 C1B M:BCL402 3.1 33.1 1.0 C1C M:BCL402 3.1 36.4 1.0 C1D M:BCL402 3.1 36.6 1.0 C4A M:BCL402 3.1 33.0 1.0 C4C M:BCL402 3.2 37.3 1.0 C1A M:BCL402 3.2 33.9 1.0 NE2 M:HIS202 3.3 34.2 1.0 CHC M:BCL402 3.4 35.5 1.0 CBB L:BCL303 3.4 41.7 1.0 CHB M:BCL402 3.4 32.6 1.0 CHA M:BCL402 3.4 34.5 1.0 CHD M:BCL402 3.5 37.5 1.0 CAB L:BCL303 4.0 42.6 1.0 ND1 M:HIS202 4.1 34.7 1.0 C3D M:BCL402 4.2 36.1 1.0 C2B M:BCL402 4.3 32.8 1.0 C2D M:BCL402 4.3 36.9 1.0 C3B M:BCL402 4.3 33.3 1.0 C2C M:BCL402 4.4 37.5 1.0 C3A M:BCL402 4.4 32.4 1.0 C3C M:BCL402 4.5 38.1 1.0 C3B L:BCL303 4.5 43.8 1.0 C2A M:BCL402 4.5 33.0 1.0 OBB L:BCL303 4.5 43.2 1.0 CD2 M:HIS202 4.6 34.5 1.0 CBD M:BCL402 4.9 34.6 1.0 CD1 M:ILE206 5.0 31.1 1.0 CMC M:BCL402 5.0 37.8 1.0

J.M.Schieferstein, A.S.Pawate, C.Sun, F.Wan, P.N.Sheraden, J.Broecker, O.P.Ernst, R.B.Gennis, P.J.A.Kenis. X-Ray Transparent Microfluidic Chips For High-Throughput Screening and Optimization of in Meso Membrane Protein Crystallization. Biomicrofluidics V. 11 24118 2017.
ISSN: ESSN 1932-1058
PubMed: 28469762
DOI: 10.1063/1.4981818