Magnesium in PDB 5wjr: High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant

All present enzymatic activity of High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant:
3.1.26.4;

The structure of High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant, PDB code: 5wjr was solved by Z.Fang, Z.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	61.79 / 1.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	80.264, 37.644, 62.121, 90.00, 95.95, 90.00
R / Rfree (%)	18.9 / 23.4

The binding sites of Magnesium atom in the High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant (pdb code 5wjr). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant, PDB code: 5wjr:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:15.2 occ:1.00 OD1 A:ASN132 2.2 12.3 1.0 OD1 A:ASP71 2.2 9.9 1.0 OE2 A:GLU109 2.4 11.3 1.0 O3' B:A6 2.6 10.1 1.0 ND2 A:ASN132 2.7 11.4 1.0 CG A:ASN132 2.8 9.8 1.0 MG A:MG201 2.8 36.5 1.0 O A:HOH325 3.0 10.9 1.0 CG A:ASP71 3.2 10.9 1.0 CD A:GLU109 3.2 8.6 1.0 OE1 A:GLU109 3.4 9.4 1.0 OD2 A:ASP71 3.6 11.4 1.0 C3' B:A6 3.8 10.1 1.0 C4' B:A6 4.0 10.7 1.0 O2' B:A6 4.1 9.5 1.0 CB A:ASN132 4.2 8.5 1.0 CB A:ASP71 4.4 9.0 1.0 C2' B:A6 4.5 9.9 1.0 CG A:GLU109 4.6 7.3 1.0 C5' B:A6 4.6 11.0 1.0 N A:VAL72 4.6 9.4 1.0 CA A:ASP71 4.7 9.6 1.0 CG1 A:VAL72 4.7 13.6 1.0 N A:ASN132 4.8 6.9 1.0 CA A:ASN132 4.8 7.3 1.0 C A:ASN132 4.9 7.2 1.0 CG2 A:ILE189 5.0 11.2 1.0

Magnesium binding site 2 out of 2 in the High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of High Resolution Native Hexamer Dna and Rna Hybrid in Complex with Rnase H Catalytic Domain D132N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:36.5 occ:1.00 OD2 A:ASP71 2.7 11.4 1.0 ND2 A:ASN132 2.7 11.4 1.0 MG B:MG201 2.8 15.2 1.0 O3' B:A6 3.2 10.1 1.0 OD1 A:ASP71 3.2 9.9 1.0 CG A:ASP71 3.3 10.9 1.0 OD1 A:ASP192 3.3 30.5 1.0 OE1 A:GLU188 3.4 49.3 1.0 CG A:ASN132 3.8 9.8 1.0 O A:HOH313 3.8 23.7 1.0 O A:HOH324 4.1 12.1 1.0 OD1 A:ASN132 4.1 12.3 1.0 CG2 A:ILE189 4.2 11.2 1.0 CG A:ASP192 4.3 28.7 1.0 C3' B:A6 4.4 10.1 1.0 CD A:GLU188 4.5 41.7 1.0 CB A:ASP192 4.7 25.0 1.0 CB A:ASP71 4.7 9.0 1.0 OE2 A:GLU188 4.8 47.5 1.0 OE2 A:GLU109 4.9 11.3 1.0 C5' B:A6 4.9 11.0 1.0 C4' B:A6 4.9 10.7 1.0 CA A:ASP192 5.0 21.3 1.0 O A:HOH325 5.0 10.9 1.0

Z.Fang, Z.Huang. Rnase H Catalytic Domain D132N Mutant in Complex with Native Dna/Rna at 1.70 Angstroms To Be Published.