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Magnesium in PDB 5xim: Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

Enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

All present enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites:
5.3.1.5;

Protein crystallography data

The structure of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 5xim was solved by J.Janin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.60
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 143.450, 143.450, 231.500, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites (pdb code 5xim). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 5xim:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in 5xim

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Magnesium binding site 1 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg395

b:16.3
occ:1.00
 OE2 A:GLU181 2.1 17.1 1.0
OD2 A:ASP245 2.1 19.5 1.0
OE1 A:GLU217 2.1 21.3 1.0
OD2 A:ASP292 2.1 13.6 1.0
O4 A:SOR397 2.2 20.1 1.0
O2 A:SOR397 2.3 14.4 1.0
CD A:GLU181 3.0 15.7 1.0
CG A:ASP292 3.1 13.0 1.0
OE1 A:GLU181 3.2 16.1 1.0
CG A:ASP245 3.2 16.8 1.0
CD A:GLU217 3.3 21.4 1.0
C4 A:SOR397 3.4 20.7 1.0
C2 A:SOR397 3.5 16.0 1.0
CB A:ASP245 3.8 14.4 1.0
C3 A:SOR397 3.8 18.8 1.0
CB A:ASP292 3.8 11.1 1.0
O3 A:SOR397 3.8 19.5 1.0
OE2 A:GLU217 4.1 24.3 1.0
CG A:GLU217 4.2 19.3 1.0
OD1 A:ASP292 4.2 14.3 1.0
CE1 A:HIS220 4.2 13.8 1.0
CB A:GLU217 4.2 16.3 1.0
OD1 A:ASP245 4.2 17.6 1.0
O A:HOH536 4.2 30.2 1.0
CG A:GLU181 4.4 12.6 1.0
NE2 A:HIS220 4.5 13.5 1.0
O6 A:SOR397 4.6 29.1 1.0
C5 A:SOR397 4.7 21.3 1.0
ND2 A:ASN215 4.7 6.6 1.0
C1 A:SOR397 4.7 14.8 1.0
O1 A:SOR397 5.0 16.6 1.0

Magnesium binding site 2 out of 4 in 5xim

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Magnesium binding site 2 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg395

b:17.6
occ:1.00
 OE1 B:GLU217 2.0 18.6 1.0
OD2 B:ASP245 2.1 19.9 1.0
O4 B:SOR397 2.2 25.1 1.0
OD2 B:ASP292 2.2 17.5 1.0
O2 B:SOR397 2.2 23.9 1.0
OE2 B:GLU181 2.3 15.5 1.0
CD B:GLU181 3.0 16.3 1.0
OE1 B:GLU181 3.1 15.8 1.0
CD B:GLU217 3.2 18.4 1.0
CG B:ASP245 3.2 18.1 1.0
CG B:ASP292 3.2 17.3 1.0
C4 B:SOR397 3.3 24.8 1.0
C2 B:SOR397 3.5 24.8 1.0
O3 B:SOR397 3.7 23.4 1.0
C3 B:SOR397 3.7 24.6 1.0
OE2 B:GLU217 3.8 22.3 1.0
CB B:ASP292 3.8 13.6 1.0
CB B:ASP245 3.9 15.4 1.0
OD1 B:ASP245 4.1 19.3 1.0
CE1 B:HIS220 4.2 17.1 1.0
CG B:GLU217 4.2 17.3 1.0
CB B:GLU217 4.2 15.0 1.0
OD1 B:ASP292 4.3 21.1 1.0
O B:HOH532 4.4 43.7 1.0
CG B:GLU181 4.4 16.0 1.0
O6 B:SOR397 4.5 29.6 1.0
NE2 B:HIS220 4.6 17.6 1.0
C5 B:SOR397 4.6 24.6 1.0
C1 B:SOR397 4.6 24.7 1.0
ND2 B:ASN215 4.8 13.7 1.0
C6 B:SOR397 4.9 25.6 1.0
ND1 B:HIS220 5.0 17.3 1.0

Magnesium binding site 3 out of 4 in 5xim

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Magnesium binding site 3 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg395

b:11.0
occ:1.00
 OD2 C:ASP245 2.1 20.9 1.0
OE1 C:GLU217 2.1 18.6 1.0
O2 C:SOR397 2.2 20.7 1.0
OE2 C:GLU181 2.2 18.4 1.0
O4 C:SOR397 2.2 17.9 1.0
OD2 C:ASP292 2.2 17.4 1.0
CD C:GLU181 3.0 17.7 1.0
OE1 C:GLU181 3.2 18.9 1.0
CG C:ASP245 3.3 17.2 1.0
CG C:ASP292 3.3 16.3 1.0
CD C:GLU217 3.4 18.7 1.0
C4 C:SOR397 3.4 19.0 1.0
C2 C:SOR397 3.4 19.9 1.0
C3 C:SOR397 3.8 19.7 1.0
CB C:ASP245 3.8 12.8 1.0
CB C:ASP292 3.8 13.2 1.0
O3 C:SOR397 3.9 20.8 1.0
CE1 C:HIS220 4.1 12.9 1.0
OD1 C:ASP245 4.2 17.2 1.0
CG C:GLU217 4.2 14.1 1.0
OE2 C:GLU217 4.2 22.6 1.0
O C:HOH553 4.2 27.0 1.0
CB C:GLU217 4.3 11.1 1.0
CG C:GLU181 4.4 16.6 1.0
OD1 C:ASP292 4.4 16.7 1.0
NE2 C:HIS220 4.5 12.6 1.0
O6 C:SOR397 4.5 27.4 1.0
ND2 C:ASN215 4.6 10.1 1.0
C1 C:SOR397 4.6 19.4 1.0
C5 C:SOR397 4.7 19.7 1.0
C6 C:SOR397 4.9 21.4 1.0
ND1 C:HIS220 5.0 12.2 1.0

Magnesium binding site 4 out of 4 in 5xim

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Magnesium binding site 4 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg395

b:16.0
occ:1.00
 OE1 D:GLU217 2.1 15.8 1.0
O4 D:SOR397 2.1 20.5 1.0
OD2 D:ASP245 2.1 21.9 1.0
OE2 D:GLU181 2.1 16.9 1.0
OD2 D:ASP292 2.1 13.4 1.0
O2 D:SOR397 2.3 17.6 1.0
CD D:GLU181 3.1 16.5 1.0
CG D:ASP292 3.2 13.6 1.0
CG D:ASP245 3.2 20.2 1.0
CD D:GLU217 3.3 16.2 1.0
C4 D:SOR397 3.3 20.2 1.0
C2 D:SOR397 3.4 17.9 1.0
OE1 D:GLU181 3.4 16.5 1.0
C3 D:SOR397 3.7 19.6 1.0
CB D:ASP292 3.7 12.7 1.0
O3 D:SOR397 3.7 21.2 1.0
CB D:ASP245 3.7 17.9 1.0
OE2 D:GLU217 4.0 20.4 1.0
CG D:GLU217 4.1 14.2 1.0
CB D:GLU217 4.2 13.2 1.0
CE1 D:HIS220 4.2 10.1 1.0
OD1 D:ASP245 4.2 20.3 1.0
OD1 D:ASP292 4.3 14.8 1.0
O D:HOH546 4.3 29.6 1.0
CG D:GLU181 4.4 15.6 1.0
NE2 D:HIS220 4.5 11.3 1.0
C5 D:SOR397 4.6 20.5 1.0
ND2 D:ASN215 4.7 10.8 1.0
C1 D:SOR397 4.7 16.9 1.0
O6 D:SOR397 4.7 26.2 1.0

Reference:

J.Jenkins, J.Janin, F.Rey, M.Chiadmi, H.Van Tilbeurgh, I.Lasters, M.De Maeyer, D.Van Belle, S.J.Wodak, M.Lauwereys, P.Stanssens, G.Matthyssens, A.M.Lambeir. Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site-Directed Mutagenesis of Metal Binding Sites. Biochemistry V. 31 5449 1992.
ISSN: ISSN 0006-2960
PubMed: 1610791
DOI: 10.1021/BI00139A005
Page generated: Mon Sep 30 09:28:12 2024

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