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Magnesium in PDB 5xin: Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

Enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites

All present enzymatic activity of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites:
5.3.1.5;

Protein crystallography data

The structure of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 5xin was solved by J.Janin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.30
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 143.450, 143.450, 231.500, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites (pdb code 5xin). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites, PDB code: 5xin:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in 5xin

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Magnesium binding site 1 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg395

b:30.9
occ:1.00
 OD2 A:ASP292 2.0 13.2 1.0
OD2 A:ASP245 2.1 18.3 1.0
OE2 A:GLU181 2.1 16.9 1.0
OE1 A:GLU217 2.1 17.4 1.0
O2 A:XLS397 2.3 31.4 1.0
O4 A:XLS397 2.4 32.1 1.0
CD A:GLU181 3.0 15.8 1.0
CG A:ASP292 3.1 12.2 1.0
OE1 A:GLU181 3.2 18.1 1.0
CG A:ASP245 3.2 15.0 1.0
CD A:GLU217 3.3 16.8 1.0
C4 A:XLS397 3.5 33.0 1.0
C2 A:XLS397 3.5 32.6 1.0
C3 A:XLS397 3.8 33.0 1.0
CB A:ASP292 3.8 11.2 1.0
CB A:ASP245 3.8 11.8 1.0
O3 A:XLS397 3.8 33.1 1.0
O A:HOH454 3.9 17.2 1.0
OE2 A:GLU217 4.0 19.3 1.0
OD1 A:ASP292 4.1 12.3 1.0
CG A:GLU217 4.2 15.2 1.0
CE1 A:HIS220 4.2 11.4 1.0
OD1 A:ASP245 4.2 16.1 1.0
CB A:GLU217 4.2 12.7 1.0
O A:HOH539 4.2 26.2 1.0
CG A:GLU181 4.4 14.2 1.0
NE2 A:HIS220 4.6 10.5 1.0
ND2 A:ASN215 4.7 6.1 1.0
C1 A:XLS397 4.7 32.5 1.0
C5 A:XLS397 4.7 31.8 1.0

Magnesium binding site 2 out of 4 in 5xin

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Magnesium binding site 2 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg395

b:26.8
occ:1.00
 OE1 B:GLU217 2.0 15.1 1.0
OD2 B:ASP245 2.0 18.4 1.0
OD2 B:ASP292 2.1 15.3 1.0
OE2 B:GLU181 2.2 21.1 1.0
O2 B:XLS397 2.3 38.3 1.0
O4 B:XLS397 2.3 35.2 1.0
CD B:GLU181 3.0 19.4 1.0
OE1 B:GLU181 3.0 20.0 1.0
CD B:GLU217 3.2 13.8 1.0
CG B:ASP245 3.2 15.1 1.0
CG B:ASP292 3.2 13.5 1.0
C4 B:XLS397 3.4 37.5 1.0
C2 B:XLS397 3.5 38.7 1.0
O B:HOH458 3.7 20.5 1.0
C3 B:XLS397 3.7 38.0 1.0
O3 B:XLS397 3.8 37.9 1.0
CB B:ASP292 3.9 12.2 1.0
CB B:ASP245 3.9 12.5 1.0
OE2 B:GLU217 3.9 18.4 1.0
CG B:GLU217 4.1 13.3 1.0
OD1 B:ASP245 4.2 13.3 1.0
O B:HOH538 4.2 22.5 1.0
CB B:GLU217 4.2 11.8 1.0
CE1 B:HIS220 4.2 11.2 1.0
OD1 B:ASP292 4.3 13.7 1.0
CG B:GLU181 4.4 18.5 1.0
C5 B:XLS397 4.6 36.1 1.0
C1 B:XLS397 4.7 39.4 1.0
ND2 B:ASN215 4.7 13.8 1.0
NE2 B:HIS220 4.7 12.3 1.0
O1 B:XLS397 5.0 41.1 1.0

Magnesium binding site 3 out of 4 in 5xin

Go back to Magnesium Binding Sites List in 5xin
Magnesium binding site 3 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg395

b:22.7
occ:1.00
 OE1 C:GLU217 2.0 15.2 1.0
OD2 C:ASP292 2.1 11.7 1.0
OD2 C:ASP245 2.1 18.0 1.0
OE2 C:GLU181 2.2 19.7 1.0
O2 C:XLS397 2.3 32.8 1.0
O4 C:XLS397 2.5 27.4 1.0
CD C:GLU181 3.1 17.4 1.0
CG C:ASP292 3.2 10.6 1.0
CD C:GLU217 3.2 14.2 1.0
OE1 C:GLU181 3.2 19.4 1.0
CG C:ASP245 3.2 14.4 1.0
C4 C:XLS397 3.6 28.9 1.0
C2 C:XLS397 3.6 33.1 1.0
CB C:ASP292 3.8 9.0 1.0
CB C:ASP245 3.8 11.8 1.0
C3 C:XLS397 3.9 31.3 1.0
OE2 C:GLU217 3.9 17.8 1.0
O3 C:XLS397 4.0 32.1 1.0
O C:HOH467 4.0 22.2 1.0
CG C:GLU217 4.1 12.1 1.0
O C:HOH551 4.2 25.3 1.0
CB C:GLU217 4.2 9.5 1.0
OD1 C:ASP245 4.2 15.0 1.0
OD1 C:ASP292 4.2 9.8 1.0
CE1 C:HIS220 4.2 9.3 1.0
CG C:GLU181 4.4 16.0 1.0
ND2 C:ASN215 4.6 6.0 1.0
C1 C:XLS397 4.6 33.8 1.0
NE2 C:HIS220 4.6 8.6 1.0
C5 C:XLS397 4.8 26.1 1.0
O1 C:XLS397 4.9 36.5 1.0

Magnesium binding site 4 out of 4 in 5xin

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Magnesium binding site 4 out of 4 in the Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site- Directed Mutagenesis of Metal Binding Sites within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg395

b:26.0
occ:1.00
 OD2 D:ASP292 2.0 11.5 1.0
OE1 D:GLU217 2.0 12.8 1.0
OD2 D:ASP245 2.0 19.0 1.0
OE2 D:GLU181 2.2 19.8 1.0
O4 D:XLS397 2.4 36.0 1.0
O2 D:XLS397 2.4 35.0 1.0
CG D:ASP292 3.1 10.1 1.0
CD D:GLU181 3.1 16.9 1.0
CG D:ASP245 3.2 16.3 1.0
CD D:GLU217 3.2 14.0 1.0
OE1 D:GLU181 3.4 18.8 1.0
C4 D:XLS397 3.5 36.2 1.0
C2 D:XLS397 3.6 36.7 1.0
O3 D:XLS397 3.7 37.8 1.0
CB D:ASP292 3.7 9.9 1.0
CB D:ASP245 3.8 14.8 1.0
C3 D:XLS397 3.8 36.5 1.0
O D:HOH469 3.9 23.8 1.0
OE2 D:GLU217 4.0 19.2 1.0
O D:HOH549 4.0 26.9 1.0
CG D:GLU217 4.1 12.0 1.0
OD1 D:ASP292 4.1 9.4 1.0
CB D:GLU217 4.2 10.6 1.0
OD1 D:ASP245 4.2 13.4 1.0
CE1 D:HIS220 4.3 7.7 1.0
CG D:GLU181 4.4 15.3 1.0
ND2 D:ASN215 4.6 8.2 1.0
NE2 D:HIS220 4.7 8.2 1.0
C1 D:XLS397 4.7 36.8 1.0
C5 D:XLS397 4.7 34.6 1.0

Reference:

J.Jenkins, J.Janin, F.Rey, M.Chiadmi, H.Van Tilbeurgh, I.Lasters, M.De Maeyer, D.Van Belle, S.J.Wodak, M.Lauwereys, P.Stanssens, G.Matthyssens, A.M.Lambeir. Protein Engineering of Xylose (Glucose) Isomerase From Actinoplanes Missouriensis. 1. Crystallography and Site-Directed Mutagenesis of Metal Binding Sites. Biochemistry V. 31 5449 1992.
ISSN: ISSN 0006-2960
PubMed: 1610791
DOI: 10.1021/BI00139A005
Page generated: Mon Sep 30 09:28:16 2024

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