Magnesium in PDB 5xn9: Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample)

The structure of Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample), PDB code: 5xn9 was solved by Y.Fukuda, Y.Miura, E.Mizohata, T.Inoue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.26 / 1.45
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.866, 57.505, 111.723, 90.00, 90.00, 90.00
R / Rfree (%)	14.5 / 17.2

The structure of Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample) also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Zinc	(Zn)	4 atoms

The binding sites of Magnesium atom in the Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample) (pdb code 5xn9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample), PDB code: 5xn9:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:25.2 occ:0.70 OXT A:ACY210 1.9 46.6 1.0 NE2 A:HIS72 1.9 21.9 1.0 OH A:TYR163 2.0 27.2 1.0 C A:ACY210 2.3 48.1 1.0 O A:ACY210 2.7 46.8 1.0 CD2 A:HIS72 2.9 21.6 1.0 CE1 A:HIS72 2.9 21.8 1.0 CZ A:TYR163 3.0 23.5 1.0 OH A:TYR152 3.1 36.3 1.0 CH3 A:ACY210 3.2 48.9 1.0 CG2 A:THR70 3.6 21.5 1.0 NZ A:LYS150 3.7 42.2 1.0 CE2 A:TYR163 3.8 21.9 1.0 CE1 A:TYR163 3.8 22.7 1.0 CD A:LYS150 3.8 36.3 1.0 CG A:HIS72 4.0 19.3 1.0 ND1 A:HIS72 4.0 21.5 1.0 CZ A:TYR152 4.3 35.0 1.0 NH1 A:ARG161 4.3 34.1 1.0 CE A:LYS150 4.3 40.4 1.0 CE2 A:TYR152 4.6 33.8 1.0 CB A:THR70 4.6 21.1 1.0 CD A:ARG161 5.0 33.7 1.0 CD2 A:TYR163 5.0 21.6 1.0 CD1 A:TYR163 5.0 21.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of A Secretary Abundant Heat Soluble (Sahs) Protein From Ramazzottius Varieornatus (From Monomer Sample) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:33.4 occ:0.56 OH B:TYR163 1.8 27.4 1.0 O B:ACY213 2.0 49.1 1.0 NE2 B:HIS72 2.1 24.6 1.0 C B:ACY213 2.4 50.3 1.0 OXT B:ACY213 2.5 49.4 1.0 OH B:TYR152 2.5 30.0 1.0 CZ B:TYR163 2.9 22.8 1.0 CD2 B:HIS72 3.1 26.6 1.0 CE1 B:HIS72 3.1 25.7 1.0 CE1 B:TYR163 3.6 21.8 1.0 NZ B:LYS150 3.7 29.5 0.7 CH3 B:ACY213 3.7 51.1 1.0 CD B:LYS150 3.7 23.6 0.7 CE2 B:TYR163 3.7 21.4 1.0 CZ B:TYR152 3.8 25.5 1.0 NZ B:LYS150 3.8 20.8 0.3 CD B:LYS150 3.8 18.2 0.3 CG2 B:THR70 4.0 26.4 1.0 CE B:LYS150 4.2 26.8 0.7 CE2 B:TYR152 4.2 26.7 1.0 CG B:HIS72 4.2 22.7 1.0 ND1 B:HIS72 4.2 20.3 1.0 CE B:LYS150 4.2 20.2 0.3 NH1 B:ARG161 4.4 31.3 1.0 CD B:ARG161 4.7 28.3 1.0 CB B:THR70 4.8 25.7 1.0 CD1 B:TYR163 4.9 19.5 1.0 CE1 B:TYR152 4.9 22.8 1.0 CG B:LYS150 4.9 22.1 0.7 CD2 B:TYR163 4.9 19.3 1.0 CG B:LYS150 5.0 16.2 0.3

Y.Fukuda, Y.Miura, E.Mizohata, T.Inoue. Structural Insights Into A Secretory Abundant Heat-Soluble Protein From An Anhydrobiotic Tardigrade, Ramazzottius Varieornatus Febs Lett. V. 591 2458 2017.
ISSN: ISSN 1873-3468
PubMed: 28703282
DOI: 10.1002/1873-3468.12752