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Magnesium in PDB 5y34: Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition

Enzymatic activity of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition

All present enzymatic activity of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition:
1.12.5.1;

Protein crystallography data

The structure of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition, PDB code: 5y34 was solved by Y.Shomura, K.S.Yoon, H.Nishihara, Y.Higuchi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.89 / 1.32
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 75.628, 116.943, 113.337, 90.00, 91.42, 90.00
R / Rfree (%) 13.1 / 15.8

Other elements in 5y34:

The structure of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition also contains other interesting chemical elements:

Nickel (Ni) 2 atoms
Iron (Fe) 26 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition (pdb code 5y34). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition, PDB code: 5y34:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 5y34

Go back to Magnesium Binding Sites List in 5y34
Magnesium binding site 1 out of 2 in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg606

b:10.3
occ:1.00
O A:HOH729 2.0 11.4 1.0
O A:HOH752 2.1 10.5 1.0
O A:CYS542 2.1 10.0 1.0
OE2 A:GLU57 2.1 10.8 1.0
O A:HOH746 2.1 10.8 1.0
NE2 A:HIS596 2.2 9.6 1.0
CE1 A:HIS596 3.1 9.2 1.0
CD A:GLU57 3.1 10.1 1.0
CD2 A:HIS596 3.2 10.3 1.0
C A:CYS542 3.3 9.8 1.0
OE1 A:GLU57 3.4 11.0 1.0
N A:CYS542 3.7 9.9 1.0
CA A:CYS542 4.0 9.8 1.0
OE1 A:GLN541 4.1 13.2 1.0
OE2 A:GLU346 4.1 11.7 1.0
OE1 A:GLU346 4.2 11.7 1.0
ND1 A:HIS596 4.2 9.3 1.0
NZ A:LYS399 4.3 12.3 1.0
O A:HOH784 4.3 10.7 1.0
CB A:CYS542 4.3 10.3 1.0
O A:HOH1068 4.3 10.6 1.0
CG A:HIS596 4.3 8.9 1.0
N A:VAL543 4.4 9.6 1.0
CG A:GLU57 4.5 10.7 1.0
CD A:LYS399 4.5 11.3 1.0
CD A:GLU346 4.6 10.7 1.0
CE A:LYS399 4.7 11.6 1.0
CA A:VAL543 4.7 9.5 1.0
C A:GLN541 4.8 9.8 1.0
CG2 A:VAL543 5.0 12.1 1.0

Magnesium binding site 2 out of 2 in 5y34

Go back to Magnesium Binding Sites List in 5y34
Magnesium binding site 2 out of 2 in the Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Membrane-Bound Respiratory [Nife]-Hydrogenase From Hydrogenovibrio Marinus in A Ferricyanide-Oxidized Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg701

b:12.8
occ:1.00
O C:HOH817 2.1 13.6 1.0
OE2 C:GLU57 2.1 12.8 1.0
O C:HOH831 2.1 13.2 1.0
O C:HOH829 2.1 13.3 1.0
O C:CYS542 2.1 12.1 1.0
NE2 C:HIS596 2.2 12.7 1.0
CD C:GLU57 3.1 12.4 1.0
CE1 C:HIS596 3.1 11.1 1.0
CD2 C:HIS596 3.2 13.7 1.0
C C:CYS542 3.3 11.8 1.0
OE1 C:GLU57 3.4 13.3 1.0
N C:CYS542 3.7 12.1 1.0
CA C:CYS542 4.0 11.6 1.0
OE1 C:GLN541 4.1 14.4 1.0
OE2 C:GLU346 4.1 13.8 1.0
OE1 C:GLU346 4.1 13.4 1.0
ND1 C:HIS596 4.2 11.6 1.0
NZ C:LYS399 4.3 14.3 1.0
O C:HOH840 4.3 12.8 1.0
CB C:CYS542 4.3 11.5 1.0
O C:HOH1119 4.3 12.9 1.0
CG C:HIS596 4.3 11.7 1.0
N C:VAL543 4.4 11.9 1.0
CG C:GLU57 4.4 12.4 1.0
CD C:LYS399 4.5 12.7 1.0
CD C:GLU346 4.6 13.7 1.0
CE C:LYS399 4.7 12.9 1.0
CA C:VAL543 4.7 12.1 1.0
C C:GLN541 4.8 11.5 1.0
CG2 C:VAL543 5.0 13.3 1.0

Reference:

Y.Shomura, K.S.Yoon, H.Nishihara, Y.Higuchi. Structural Basis For A [4FE-3S] Cluster in the Oxygen-Tolerant Membrane-Bound [Nife]-Hydrogenase Nature V. 479 253 2011.
ISSN: ESSN 1476-4687
PubMed: 22002607
DOI: 10.1038/NATURE10504
Page generated: Mon Sep 30 10:44:34 2024

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