Atomistry » Magnesium » PDB 6asw-6b4k » 6at2
Atomistry »
  Magnesium »
    PDB 6asw-6b4k »
      6at2 »

Magnesium in PDB 6at2: E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate

Enzymatic activity of E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate

All present enzymatic activity of E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate:
4.1.1.49;

Protein crystallography data

The structure of E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate, PDB code: 6at2 was solved by H.Y.H.Tang, D.S.Shin, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.18 / 1.44
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 125.171, 94.360, 46.559, 90.00, 96.40, 90.00
R / Rfree (%) 17.1 / 19.1

Other elements in 6at2:

The structure of E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate also contains other interesting chemical elements:

Manganese (Mn) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate (pdb code 6at2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate, PDB code: 6at2:

Magnesium binding site 1 out of 1 in 6at2

Go back to Magnesium Binding Sites List in 6at2
Magnesium binding site 1 out of 1 in the E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of E. Coli Phosphoenolpyruvate Carboxykinase G209N Mutant Bound to Thiosulfate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg703

b:7.5
occ:1.00
 O2B A:ATP701 2.1 10.9 1.0
O2G A:ATP701 2.1 13.2 1.0
O A:HOH840 2.1 12.1 1.0
O A:HOH814 2.2 13.7 1.0
O A:HOH805 2.2 12.6 1.0
OG1 A:THR255 2.2 11.5 1.0
HB A:THR255 3.1 12.5 1.0
PG A:ATP701 3.2 11.9 1.0
CB A:THR255 3.2 10.4 1.0
PB A:ATP701 3.3 11.0 1.0
H A:THR255 3.4 12.0 1.0
HH12 A:ARG333 3.5 12.0 1.0
O3B A:ATP701 3.6 13.9 1.0
O3G A:ATP701 3.7 15.1 1.0
O A:TYR286 3.8 11.9 1.0
OD2 A:ASP268 3.8 16.1 1.0
O1A A:ATP701 4.0 16.0 1.0
HB2 A:LYS254 4.0 13.6 1.0
N A:THR255 4.0 10.0 1.0
HB2 A:ASP269 4.1 14.3 1.0
HE2 A:LYS254 4.1 13.4 1.0
HG21 A:THR255 4.1 16.4 1.0
OD1 A:ASP269 4.1 12.1 1.0
O A:ASP268 4.1 13.6 1.0
CA A:THR255 4.2 11.2 1.0
NH1 A:ARG333 4.2 10.0 1.0
O3A A:ATP701 4.2 11.2 1.0
O A:HOH1010 4.2 13.4 1.0
CG2 A:THR255 4.3 13.7 1.0
HH11 A:ARG333 4.3 12.0 1.0
O1B A:ATP701 4.5 11.3 1.0
O1G A:ATP701 4.5 10.8 1.0
HA A:THR255 4.5 13.5 1.0
PA A:ATP701 4.5 14.3 1.0
HA A:ALA287 4.6 13.7 1.0
HB2 A:ASP268 4.6 23.1 1.0
HG23 A:THR255 4.6 16.4 1.0
CG A:ASP268 4.7 14.6 1.0
HZ1 A:LYS254 4.7 14.6 1.0
C A:TYR286 4.8 13.4 1.0
CG A:ASP269 4.8 15.2 1.0
CB A:ASP269 4.8 11.9 1.0
H A:TYR286 4.9 13.7 1.0
O2A A:ATP701 4.9 14.5 1.0
HZ3 A:LYS254 4.9 14.6 1.0
CB A:LYS254 4.9 11.4 1.0
HB3 A:TYR286 4.9 13.0 1.0
MN A:MN702 5.0 12.7 1.0
CE A:LYS254 5.0 11.2 1.0

Reference:

H.Y.H.Tang, D.S.Shin, G.L.Hura, Y.Yang, X.Hu, F.C.Lightstone, M.D.Mcgee, H.S.Padgett, S.M.Yannone, J.A.Tainer. Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase. Biochemistry V. 57 6688 2018.
ISSN: ISSN 1520-4995
PubMed: 30376300
DOI: 10.1021/ACS.BIOCHEM.8B00963
Page generated: Mon Sep 30 19:22:06 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy