Magnesium in PDB 6bc1: A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog

The structure of A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog, PDB code: 6bc1 was solved by Z.Chen, P.C.Sternweis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.17 / 2.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	45.332, 96.333, 189.361, 90.00, 90.00, 90.00
R / Rfree (%)	27.8 / 32.3

The binding sites of Magnesium atom in the A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog (pdb code 6bc1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog, PDB code: 6bc1:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:39.2 occ:1.00 HOG2 A:GSP201 1.7 57.0 1.0 OG1 A:THR17 1.9 35.3 1.0 O A:HOH303 2.1 24.1 1.0 HG23 A:THR35 2.1 56.9 1.0 OG1 A:THR35 2.2 29.9 1.0 O A:HOH314 2.2 32.9 1.0 O2G A:GSP201 2.2 47.5 1.0 O2B A:GSP201 2.3 28.9 1.0 HG21 A:THR35 2.5 56.9 1.0 CG2 A:THR35 2.6 47.4 1.0 CB A:THR35 2.9 32.8 1.0 H A:THR35 2.9 50.3 1.0 CB A:THR17 3.2 27.4 1.0 HB A:THR17 3.3 32.9 1.0 N A:THR35 3.4 41.9 1.0 PG A:GSP201 3.5 53.0 1.0 HG22 A:THR35 3.5 56.9 1.0 H A:THR17 3.5 36.7 1.0 OD2 A:ASP57 3.5 26.7 1.0 PB A:GSP201 3.6 34.8 1.0 HB A:THR35 3.6 39.3 1.0 CA A:THR35 3.7 42.3 1.0 HG21 A:THR17 3.8 32.6 1.0 O3B A:GSP201 3.9 27.9 1.0 CG2 A:THR17 4.0 27.1 1.0 O3G A:GSP201 4.0 32.0 1.0 HA A:PRO34 4.0 52.5 1.0 OD1 A:ASP57 4.1 38.9 1.0 N A:THR17 4.1 30.6 1.0 CG A:ASP57 4.2 26.7 1.0 CA A:THR17 4.2 25.6 1.0 HG23 A:THR17 4.2 32.6 1.0 HA A:THR35 4.3 50.8 1.0 O1A A:GSP201 4.4 43.3 1.0 O1B A:GSP201 4.4 45.2 1.0 HOG3 A:GSP201 4.4 38.4 1.0 HB2 A:LYS16 4.4 34.8 1.0 C A:PRO34 4.5 33.3 1.0 HA A:THR17 4.5 30.7 1.0 O A:ILE33 4.6 47.8 1.0 O A:THR58 4.6 22.4 1.0 HE2 A:LYS16 4.6 32.0 1.0 CA A:PRO34 4.7 43.7 1.0 O3A A:GSP201 4.7 38.7 1.0 C A:THR35 4.8 33.5 1.0 HG22 A:THR17 4.9 32.6 1.0 S1G A:GSP201 4.9 61.8 1.0 PA A:GSP201 4.9 33.2 1.0 O2A A:GSP201 4.9 25.6 1.0

Magnesium binding site 2 out of 2 in the A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of A Complex Between pH Domain of P190RHOGEF and Activated RAC1 Bound to A Gtp Analog within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg202 b:72.0 occ:1.00 OG1 B:THR17 1.9 70.2 1.0 O2G B:GSP201 2.3 0.7 1.0 O1B B:GSP201 2.3 74.1 1.0 O3G B:GSP201 2.3 92.8 1.0 OG1 B:THR35 2.3 78.3 1.0 HG23 B:THR35 2.4 78.6 1.0 HOG2 B:GSP201 2.5 0.9 1.0 HOG3 B:GSP201 2.6 0.4 1.0 HG21 B:THR35 2.6 78.6 1.0 PG B:GSP201 2.7 0.7 1.0 CG2 B:THR35 2.8 65.5 1.0 H B:THR35 3.0 0.4 1.0 CB B:THR35 3.1 75.3 1.0 OD2 B:ASP57 3.3 76.2 1.0 CB B:THR17 3.3 43.9 1.0 PB B:GSP201 3.3 96.8 1.0 O3B B:GSP201 3.4 0.6 1.0 H B:THR17 3.4 80.8 1.0 OD1 B:ASP57 3.5 36.2 1.0 HB B:THR17 3.6 52.7 1.0 CG B:ASP57 3.7 47.0 1.0 HG22 B:THR35 3.7 78.6 1.0 N B:THR35 3.8 93.7 1.0 HB B:THR35 3.8 90.4 1.0 HG21 B:THR17 4.0 56.5 1.0 N B:THR17 4.0 67.3 1.0 HB2 B:LYS16 4.0 71.7 1.0 CA B:THR35 4.0 92.5 1.0 HE2 B:LYS16 4.0 66.7 1.0 CG2 B:THR17 4.1 47.1 1.0 HG23 B:THR17 4.1 56.5 1.0 CA B:THR17 4.2 45.8 1.0 O B:THR58 4.2 49.5 1.0 O2B B:GSP201 4.2 69.9 1.0 O2A B:GSP201 4.2 92.5 1.0 S1G B:GSP201 4.2 53.5 1.0 HA B:THR17 4.3 54.9 1.0 HA B:PRO34 4.5 0.0 1.0 O3A B:GSP201 4.5 1.0 1.0 HA B:THR35 4.7 1.0 1.0 HZ1 B:LYS16 4.7 68.5 1.0 PA B:GSP201 4.7 76.8 1.0 O1A B:GSP201 4.8 68.6 1.0 C B:PRO34 4.9 91.2 1.0 CE B:LYS16 4.9 55.6 1.0 HZ3 B:LYS16 4.9 68.5 1.0 CB B:LYS16 5.0 59.8 1.0 HG22 B:THR17 5.0 56.5 1.0 HA B:ALA59 5.0 80.3 1.0 C B:THR35 5.0 66.0 1.0

O.Dada, S.Gutowski, C.A.Brautigam, Z.Chen, P.C.Sternweis. Direct Regulation of P190RHOGEF By Activated Rho and Rac Gtpases. J. Struct. Biol. V. 202 13 2018.
ISSN: ESSN 1095-8657
PubMed: 29196061
DOI: 10.1016/J.JSB.2017.11.014