Magnesium in PDB 6ca0: Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex

All present enzymatic activity of Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex:
2.7.7.6;

The structure of Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex (pdb code 6ca0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex, PDB code: 6ca0:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of E. Coli Rnap SIGMA70 Open Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2001 b:0.5 occ:1.00 O D:ASP460 2.1 0.9 1.0 OD1 D:ASP462 2.2 0.8 1.0 OD2 D:ASP464 2.2 0.3 1.0 C D:ASP460 2.6 0.9 1.0 CB D:ASP460 2.6 0.9 1.0 CA D:ASP460 2.7 0.9 1.0 N D:ASP460 2.7 0.9 1.0 CG D:ASP464 3.0 0.3 1.0 CG D:ASP462 3.1 0.8 1.0 OD2 D:ASP462 3.3 0.8 1.0 OD1 D:ASP464 3.3 0.3 1.0 N D:PHE461 3.7 0.6 1.0 CG D:ASP460 3.8 0.9 1.0 N D:ASP462 3.9 0.8 1.0 C D:ALA459 3.9 0.1 1.0 CB D:ASP464 4.2 0.3 1.0 OD2 D:ASP460 4.2 0.9 1.0 CB D:ASP462 4.4 0.8 1.0 CA D:PHE461 4.6 0.6 1.0 C D:PHE461 4.6 0.6 1.0 CA D:ALA459 4.6 0.1 1.0 CA D:ASP462 4.6 0.8 1.0 NH2 D:ARG425 4.7 0.2 1.0 OD1 D:ASP460 4.7 0.9 1.0 O D:ALA459 4.8 0.1 1.0 C D:ASP462 5.0 0.8 1.0 N D:ASP464 5.0 0.3 1.0

A.Narayanan, F.S.Vago, K.Li, M.Z.Qayyum, D.Yernool, W.Jiang, K.S.Murakami. Cryo-Em Structure Ofescherichia COLISIGMA70RNA Polymerase and Promoter Dna Complex Revealed A Role of Sigma Non-Conserved Region During the Open Complex Formation. J. Biol. Chem. V. 293 7367 2018.
ISSN: ESSN 1083-351X
PubMed: 29581236
DOI: 10.1074/JBC.RA118.002161