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Magnesium in PDB 6dof: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C, PDB code: 6dof was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 31.06 / 1.43
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.839, 37.889, 62.494, 90.00, 96.26, 90.00
R / Rfree (%) 16.1 / 18.7

Other elements in 6dof:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C (pdb code 6dof). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C, PDB code: 6dof:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dof

Go back to Magnesium Binding Sites List in 6dof
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:20.3
occ:1.00
OD2 A:ASP71 2.0 17.7 1.0
OP3 b:C5 2.0 31.8 0.6
O A:HOH302 2.0 27.8 0.5
OD1 A:ASP192 2.1 26.8 1.0
O A:HOH305 2.1 24.5 1.0
O A:HOH318 2.1 20.2 1.0
OP1 b:C5 2.3 27.1 0.5
OP1 b:C5 2.4 25.1 0.6
P b:C5 2.7 22.7 0.6
CG A:ASP71 3.0 16.5 1.0
CG A:ASP192 3.2 22.4 1.0
HA A:ASP192 3.2 23.6 1.0
OD1 A:ASP71 3.3 16.2 1.0
P b:C5 3.4 19.3 0.5
HB2 A:ASP192 3.6 25.7 1.0
H A:TYR193 3.6 26.8 1.0
MG A:MG202 3.7 17.4 1.0
CB A:ASP192 3.8 21.4 1.0
OP2 b:C5 3.8 22.2 0.6
NZ A:LYS196 3.9 22.8 0.6
O5' b:C5 3.9 19.1 0.6
OP2 b:C5 3.9 22.3 0.5
K A:K203 3.9 27.5 0.1
O b:HOH101 3.9 28.0 0.9
O A:VAL72 3.9 18.3 1.0
OP2 b:G6 3.9 34.1 1.0
CA A:ASP192 4.0 19.7 1.0
OD2 A:ASP132 4.1 35.2 1.0
OE2 A:GLU188 4.1 43.3 1.0
HG22 A:ILE189 4.2 20.7 1.0
OD2 A:ASP192 4.2 23.9 1.0
C5' b:C5 4.3 22.3 1.0
O5' b:C5 4.3 22.1 0.5
O A:HOH316 4.3 26.3 0.5
CB A:ASP71 4.3 14.9 1.0
N A:TYR193 4.4 22.3 1.0
HB3 A:ASP71 4.4 17.9 1.0
HD1 A:TYR193 4.6 23.9 1.0
O3' B:U4 4.6 27.7 0.5
HB2 A:ASP71 4.7 17.9 1.0
HG21 A:ILE189 4.7 20.7 1.0
HB3 A:ASP192 4.7 25.7 1.0
C A:ASP192 4.8 21.3 1.0
CG2 A:ILE189 4.8 17.2 1.0
K A:K217 4.9 21.5 0.3
O A:HOH324 4.9 34.7 1.0
HB2 A:TYR193 4.9 37.6 1.0
HG23 A:ILE189 5.0 20.7 1.0
H A:VAL72 5.0 22.2 1.0

Magnesium binding site 2 out of 2 in 6dof

Go back to Magnesium Binding Sites List in 6dof
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 540 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:17.4
occ:1.00
OE1 A:GLU109 2.0 16.1 1.0
OD1 A:ASP71 2.0 16.2 1.0
OP1 b:C5 2.1 25.1 0.6
OD1 A:ASP132 2.1 18.3 1.0
OP1 b:C5 2.1 27.1 0.5
O3' B:U4 2.6 27.7 0.5
OD2 A:ASP132 2.7 35.2 1.0
CG A:ASP132 2.7 19.6 1.0
P b:C5 2.9 19.3 0.5
CD A:GLU109 3.0 14.4 1.0
CG A:ASP71 3.1 16.5 1.0
O A:HOH347 3.3 15.6 1.0
OE2 A:GLU109 3.3 15.3 1.0
P b:C5 3.4 22.7 0.6
C3' B:U4 3.5 25.9 0.6
HG12 A:VAL72 3.5 24.7 1.0
OD2 A:ASP71 3.6 17.7 1.0
O3' B:U4 3.6 23.2 0.6
O A:HOH318 3.7 20.2 1.0
MG A:MG201 3.7 20.3 1.0
H A:VAL72 3.7 22.2 1.0
OP2 b:C5 3.9 22.2 0.6
O5' b:C5 3.9 22.1 0.5
C3' B:U4 3.9 35.6 0.5
HA A:ASP71 4.0 16.2 1.0
C5' b:C5 4.0 22.3 1.0
OP2 b:C5 4.1 22.3 0.5
O5' b:C5 4.2 19.1 0.6
CB A:ASP132 4.2 15.6 1.0
H A:ASP132 4.3 14.3 1.0
HB2 A:SER133 4.3 16.5 1.0
O2' B:U4 4.3 21.2 0.5
OP3 b:C5 4.3 31.8 0.6
CG A:GLU109 4.4 12.1 1.0
CB A:ASP71 4.4 14.9 1.0
C4' B:U4 4.4 28.6 0.5
C4' B:U4 4.5 28.5 0.6
HG21 A:ILE189 4.5 20.7 1.0
HG3 A:GLU109 4.5 14.6 1.0
CG1 A:VAL72 4.5 20.6 1.0
N A:VAL72 4.5 18.5 1.0
C5' B:U4 4.6 18.2 0.6
HB3 A:ASP132 4.6 18.7 1.0
HB2 A:ASP132 4.6 18.7 1.0
C2' B:U4 4.7 24.4 0.6
HB2 A:ASP71 4.7 17.9 1.0
CA A:ASP71 4.7 13.5 1.0
C2' B:U4 4.7 24.4 0.5
HG2 A:GLU109 4.7 14.6 1.0
C5' B:U4 4.7 35.1 0.5
O A:HOH305 4.7 24.5 1.0
HG13 A:VAL72 4.8 24.7 1.0
K A:K217 4.8 21.5 0.3
O A:HOH302 4.8 27.8 0.5
CA A:ASP132 4.9 13.7 1.0
HG11 A:VAL72 4.9 24.7 1.0
C A:ASP132 4.9 13.6 1.0
O A:VAL72 4.9 18.3 1.0
N A:ASP132 4.9 11.9 1.0
O2' B:U4 5.0 28.4 0.6
HG22 A:ILE189 5.0 20.7 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:18:19 2024

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