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Magnesium in PDB 6dor: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1)

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1)

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1):
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1), PDB code: 6dor was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.94 / 1.50
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.544, 37.711, 62.779, 90.00, 96.56, 90.00
R / Rfree (%) 15.5 / 19.4

Other elements in 6dor:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1) also contains other interesting chemical elements:

Potassium (K) 3 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1) (pdb code 6dor). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1), PDB code: 6dor:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dor

Go back to Magnesium Binding Sites List in 6dor
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:22.9
occ:1.00
OD1 A:ASP71 2.0 22.5 1.0
OE1 A:GLU109 2.1 24.0 1.0
OD1 A:ASP132 2.2 23.4 1.0
OP1 b:C5 2.2 26.5 0.5
OP1 b:C5 2.2 33.9 0.4
O3' B:U4 2.6 21.1 0.4
O3' B:U4 2.7 30.8 0.1
OP1 b:C5 2.8 40.2 0.1
CG A:ASP132 2.9 24.6 1.0
OD2 A:ASP132 2.9 33.4 1.0
P b:C5 3.0 22.2 0.4
CD A:GLU109 3.1 22.9 1.0
CG A:ASP71 3.1 20.8 1.0
O A:HOH334 3.3 22.8 1.0
P b:C5 3.3 22.4 0.1
OE2 A:GLU109 3.3 23.3 1.0
C3' B:U4 3.5 38.2 0.5
OD2 A:ASP71 3.5 24.3 1.0
HG12 A:VAL72 3.5 27.7 1.0
P b:C5 3.6 65.1 0.5
O3' B:U4 3.6 24.8 0.5
H A:VAL72 3.7 27.5 1.0
O b:HOH301 3.7 23.1 1.0
MG b:MG201 3.8 24.0 1.0
C3' B:U4 3.9 33.9 0.4
C3' B:U4 4.0 28.1 0.1
C5' b:C5 4.0 28.1 1.0
O5' b:C5 4.0 22.2 0.4
OP2 b:C5 4.2 24.2 0.4
OP2 b:C5 4.2 46.4 0.5
O5' b:C5 4.2 22.4 0.1
O2' B:U4 4.2 23.1 0.1
HB2 A:SER133 4.3 22.3 1.0
O5' b:C5 4.3 60.6 0.5
C4' B:U4 4.3 23.8 0.1
C4' B:U4 4.3 24.4 0.4
O2' B:U4 4.3 19.9 0.4
CB A:ASP132 4.3 18.6 1.0
CB A:ASP71 4.4 18.2 1.0
CG A:GLU109 4.4 18.1 1.0
HG21 A:ILE189 4.5 25.6 1.0
CG1 A:VAL72 4.5 23.1 1.0
C4' B:U4 4.5 27.5 0.5
N A:VAL72 4.5 22.9 1.0
C2' B:U4 4.5 33.6 0.5
OP2 b:C5 4.6 22.8 0.1
HG3 A:GLU109 4.6 21.7 1.0
C5' B:U4 4.6 31.4 0.5
OP3 b:C5 4.6 44.9 0.5
CA A:ASP71 4.6 16.7 1.0
C2' B:U4 4.7 21.4 0.4
C2' B:U4 4.7 21.9 0.1
HG2 A:GLU109 4.7 21.7 1.0
HG13 A:VAL72 4.8 27.7 1.0
O A:HOH315 4.8 25.8 1.0
C5' B:U4 4.8 21.0 0.4
O2' B:U4 4.8 38.9 0.5
C5' B:U4 4.8 21.2 0.1
HG11 A:VAL72 4.8 27.7 1.0
K A:K203 4.9 25.0 0.2
O A:VAL72 4.9 21.7 1.0
N A:ASP132 4.9 16.2 1.0
CA A:ASP132 4.9 16.4 1.0
C A:ASP132 5.0 17.7 1.0
HG22 A:ILE189 5.0 25.6 1.0

Magnesium binding site 2 out of 2 in 6dor

Go back to Magnesium Binding Sites List in 6dor
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 300 Mm K+ For 120 S at 21 C (Dataset 1) within 5.0Å range:
probe atom residue distance (Å) B Occ
b:Mg201

b:24.0
occ:1.00
OP1 b:C5 1.8 40.2 0.1
OP3 b:C5 2.0 44.9 0.5
OD2 A:ASP71 2.0 24.3 1.0
OD1 A:ASP192 2.0 29.7 1.0
O A:HOH305 2.0 29.2 0.5
O A:HOH315 2.1 25.8 1.0
O b:HOH301 2.1 23.1 1.0
OP1 b:C5 2.2 33.9 0.4
OP1 b:C5 2.2 26.5 0.5
P b:C5 2.6 65.1 0.5
CG A:ASP71 3.0 20.8 1.0
CG A:ASP192 3.2 26.0 1.0
HA A:ASP192 3.2 28.8 1.0
P b:C5 3.2 22.4 0.1
P b:C5 3.3 22.2 0.4
OD1 A:ASP71 3.4 22.5 1.0
H A:TYR193 3.6 32.0 1.0
OP2 b:C5 3.6 46.4 0.5
HB2 A:ASP192 3.7 32.6 1.0
MG A:MG201 3.8 22.9 1.0
OP2 b:C5 3.8 24.2 0.4
K A:K202 3.8 29.6 0.2
O5' b:C5 3.8 60.6 0.5
CB A:ASP192 3.8 27.2 1.0
O b:HOH302 3.9 27.5 0.4
CA A:ASP192 3.9 24.0 1.0
OP2 b:G6 4.0 30.8 1.0
OP2 b:C5 4.0 22.8 0.1
O5' b:C5 4.0 22.4 0.1
O A:VAL72 4.0 21.7 1.0
O5' b:C5 4.1 22.2 0.4
HG22 A:ILE189 4.2 25.6 1.0
OD2 A:ASP192 4.2 29.0 1.0
OD2 A:ASP132 4.2 33.4 1.0
N A:TYR193 4.3 26.7 1.0
O3' B:U4 4.3 30.8 0.1
C5' b:C5 4.4 28.1 1.0
CB A:ASP71 4.4 18.2 1.0
O3' B:U4 4.5 21.1 0.4
OE2 A:GLU188 4.5 49.4 1.0
O A:HOH317 4.5 31.3 0.6
HD1 A:TYR193 4.6 30.6 1.0
HG21 A:ILE189 4.7 25.6 1.0
C A:ASP192 4.7 25.5 1.0
HB3 A:ASP192 4.7 32.6 1.0
CG2 A:ILE189 4.8 21.3 1.0
HB2 A:TYR193 4.8 40.7 1.0
K A:K203 4.9 25.0 0.2
HG23 A:ILE189 4.9 25.6 1.0
H A:VAL72 5.0 27.5 1.0
O A:HOH356 5.0 31.8 0.6
H A:GLY194 5.0 42.4 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:21:02 2024

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