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Magnesium in PDB 6dp0: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp0 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.88 / 1.45
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.478, 37.774, 61.811, 90.00, 96.13, 90.00
R / Rfree (%) 19 / 23.6

Other elements in 6dp0:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Potassium (K) 2 atoms
Iodine (I) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C (pdb code 6dp0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp0:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dp0

Go back to Magnesium Binding Sites List in 6dp0
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:26.3
occ:1.00
OD1 A:ASP192 1.9 35.1 1.0
OD2 A:ASP71 2.0 28.1 1.0
O A:HOH335 2.1 26.3 1.0
OP1 b:C5 2.1 22.4 0.9
O A:HOH342 2.2 29.0 1.0
O A:HOH322 2.3 35.0 0.9
CG A:ASP71 3.0 18.2 1.0
CG A:ASP192 3.1 31.3 1.0
HA A:ASP192 3.3 30.8 1.0
OD1 A:ASP71 3.3 20.0 1.0
P b:C5 3.3 22.4 0.9
H A:TYR193 3.5 36.3 1.0
OP2 b:C5 3.8 22.9 0.9
HB2 A:ASP192 3.8 32.3 1.0
MG A:MG202 3.8 27.9 1.0
CB A:ASP192 3.9 26.9 1.0
K A:K203 4.0 47.5 0.3
CA A:ASP192 4.0 25.6 1.0
OP2 b:G6 4.1 28.3 1.0
OD2 A:ASP192 4.1 33.6 1.0
O A:VAL72 4.1 22.9 1.0
O5' b:C5 4.2 23.8 1.0
HG22 A:ILE189 4.2 30.8 1.0
N A:TYR193 4.3 30.3 1.0
OE2 A:GLU188 4.4 74.1 1.0
CB A:ASP71 4.4 20.8 1.0
OD2 A:ASP132 4.4 30.7 1.0
O A:HOH310 4.4 37.6 0.6
O3' B:U4 4.5 24.2 0.9
C5' b:C5 4.5 24.3 1.0
HB3 A:ASP71 4.5 25.0 1.0
HD1 A:TYR193 4.6 32.4 1.0
HB2 A:ASP71 4.7 25.0 1.0
HB2 A:TYR193 4.7 42.4 1.0
HG21 A:ILE189 4.7 30.8 1.0
C A:ASP192 4.7 28.0 1.0
OE1 A:GLU188 4.8 73.0 1.0
CG2 A:ILE189 4.8 25.7 1.0
HB3 A:ASP192 4.8 32.3 1.0
HG23 A:ILE189 4.8 30.8 1.0
O b:HOH103 4.8 43.7 1.0
H A:VAL72 4.9 26.1 1.0
CD A:GLU188 4.9 64.2 1.0

Magnesium binding site 2 out of 2 in 6dp0

Go back to Magnesium Binding Sites List in 6dp0
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:27.9
occ:1.00
OD1 A:ASP71 2.1 20.0 1.0
OE1 A:GLU109 2.4 25.5 1.0
OP1 b:C5 2.4 22.4 0.9
OD1 A:ASP132 2.4 18.1 1.0
O3' B:U4 2.7 24.2 0.9
OD2 A:ASP132 2.9 30.7 1.0
CG A:ASP132 3.0 17.8 1.0
O A:HOH370 3.1 20.2 1.0
CG A:ASP71 3.1 18.2 1.0
P b:C5 3.2 22.4 0.9
CD A:GLU109 3.2 43.6 1.0
OE2 A:GLU109 3.3 22.8 1.0
HG12 A:VAL72 3.5 27.7 1.0
OD2 A:ASP71 3.6 28.1 1.0
H A:VAL72 3.7 26.1 1.0
O A:HOH342 3.8 29.0 1.0
MG A:MG201 3.8 26.3 1.0
C3' B:U4 4.0 20.6 0.9
C5' b:C5 4.0 24.3 1.0
HA A:ASP71 4.0 17.8 1.0
O2' B:U4 4.1 19.4 1.0
O5' b:C5 4.1 23.8 1.0
C4' B:U4 4.3 26.2 1.0
H A:ASP132 4.3 19.8 1.0
OP2 b:C5 4.3 22.9 0.9
CB A:ASP71 4.4 20.8 1.0
HB2 A:SER133 4.4 19.2 1.0
HG21 A:ILE189 4.4 30.8 1.0
CG1 A:VAL72 4.4 23.1 1.0
N A:VAL72 4.5 21.7 1.0
CB A:ASP132 4.5 17.7 1.0
HB2 A:ASP71 4.6 25.0 1.0
C2' B:U4 4.6 16.4 1.0
HG13 A:VAL72 4.6 27.7 1.0
CG A:GLU109 4.6 19.7 1.0
CA A:ASP71 4.6 14.8 1.0
O A:HOH335 4.7 26.3 1.0
HG11 A:VAL72 4.8 27.7 1.0
HG3 A:GLU109 4.9 23.7 1.0
HB3 A:ASP132 4.9 21.3 1.0
O A:VAL72 4.9 22.9 1.0
HG22 A:ILE189 4.9 30.8 1.0
HG2 A:GLU109 4.9 23.7 1.0
C5' B:U4 5.0 25.1 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:23:23 2024

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