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Magnesium in PDB 6dpi: Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C, PDB code: 6dpi was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.98 / 1.35
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.620, 37.290, 62.382, 90.00, 96.98, 90.00
R / Rfree (%) 14.5 / 17.4

Other elements in 6dpi:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C also contains other interesting chemical elements:

Rubidium (Rb) 3 atoms
Chlorine (Cl) 3 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C (pdb code 6dpi). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C, PDB code: 6dpi:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dpi

Go back to Magnesium Binding Sites List in 6dpi
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:16.7
occ:1.00
O A:HOH331 2.0 19.4 1.0
OD1 A:ASP192 2.0 24.2 1.0
OD2 A:ASP71 2.0 19.0 1.0
O A:HOH375 2.1 24.2 1.0
O A:HOH318 2.1 25.0 1.0
OP1 b:C5 2.1 16.6 1.0
CG A:ASP71 3.1 15.6 1.0
CG A:ASP192 3.2 24.3 1.0
HA A:ASP192 3.3 22.8 1.0
P b:C5 3.3 15.5 0.9
OD1 A:ASP71 3.4 17.2 1.0
H A:TYR193 3.6 29.3 1.0
OP2 b:C5 3.8 19.4 1.0
CB A:ASP192 4.0 21.9 1.0
HB2 A:ASP192 4.0 26.2 1.0
MG A:MG202 4.0 14.6 1.0
OP2 b:G6 4.0 19.3 1.0
CA A:ASP192 4.0 19.0 1.0
RB A:RB203 4.1 27.2 0.3
OD2 A:ASP192 4.1 29.0 1.0
HG22 A:ILE189 4.1 25.1 1.0
O5' b:C5 4.1 15.9 1.0
O A:VAL72 4.2 15.9 1.0
OE1 A:GLU188 4.3 46.5 1.0
OD2 A:ASP132 4.3 22.0 1.0
N A:TYR193 4.3 24.4 1.0
O A:HOH343 4.4 32.8 1.0
CB A:ASP71 4.4 15.6 1.0
C5' b:C5 4.5 15.4 1.0
O3' B:U4 4.5 15.9 1.0
HB3 A:ASP71 4.5 18.7 1.0
O A:TYR193 4.6 71.9 1.0
OXT A:ALA196 4.6 60.5 0.6
HB2 A:ASP71 4.7 18.7 1.0
HG21 A:ILE189 4.7 25.1 1.0
HD1 A:TYR193 4.7 26.8 1.0
O A:HOH344 4.7 33.8 1.0
CG2 A:ILE189 4.8 20.9 1.0
C A:ASP192 4.8 22.3 1.0
HB3 A:ASP192 4.9 26.2 1.0
HG23 A:ILE189 4.9 25.1 1.0
HB2 A:TYR193 5.0 34.8 1.0
H A:VAL72 5.0 17.5 1.0

Magnesium binding site 2 out of 2 in 6dpi

Go back to Magnesium Binding Sites List in 6dpi
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:14.6
occ:1.00
OD1 A:ASP71 2.2 17.2 1.0
OD1 A:ASP132 2.2 17.5 1.0
OE1 A:GLU109 2.3 17.6 1.0
OP1 b:C5 2.6 16.6 1.0
OD2 A:ASP132 2.7 22.0 1.0
CG A:ASP132 2.8 18.0 1.0
O3' B:U4 2.8 15.9 1.0
O A:HOH373 2.9 15.8 1.0
OE2 A:GLU109 3.0 17.3 1.0
CD A:GLU109 3.0 17.8 1.0
CG A:ASP71 3.2 15.6 1.0
P b:C5 3.3 15.5 0.9
OD2 A:ASP71 3.7 19.0 1.0
HG12 A:VAL72 3.7 17.7 1.0
H A:VAL72 3.8 17.5 1.0
HA A:ASP71 3.9 16.2 1.0
MG A:MG201 4.0 16.7 1.0
O A:HOH375 4.0 24.2 1.0
C3' B:U4 4.0 15.9 1.0
H A:ASP132 4.1 16.3 1.0
C5' b:C5 4.2 15.4 1.0
O2' B:U4 4.2 18.1 1.0
HB2 A:SER133 4.2 15.8 1.0
CB A:ASP132 4.2 16.6 1.0
O5' b:C5 4.3 15.9 1.0
HG21 A:ILE189 4.3 25.1 1.0
C4' B:U4 4.4 18.2 1.0
CB A:ASP71 4.4 15.6 1.0
OP2 b:C5 4.5 19.4 1.0
CG A:GLU109 4.5 16.8 1.0
HB2 A:ASP71 4.6 18.7 1.0
CA A:ASP71 4.6 13.5 1.0
N A:VAL72 4.6 14.6 1.0
CG1 A:VAL72 4.7 14.8 1.0
HB3 A:ASP132 4.7 20.0 1.0
HB2 A:ASP132 4.8 20.0 1.0
C2' B:U4 4.8 15.1 1.0
N A:ASP132 4.8 13.6 1.0
HG3 A:GLU109 4.8 20.1 1.0
HG22 A:ILE189 4.8 25.1 1.0
HG2 A:GLU109 4.9 20.1 1.0
CA A:ASP132 4.9 14.6 1.0
O A:HOH331 4.9 19.4 1.0
C A:ASP132 4.9 14.3 1.0
C5' B:U4 5.0 18.2 1.0
HG13 A:VAL72 5.0 17.7 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:25:15 2024

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