Magnesium in PDB 6dpl: Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C, PDB code: 6dpl was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.50 / 1.45
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.856, 37.521, 62.706, 90.00, 97.31, 90.00
R / Rfree (%) 15.1 / 17.9

Other elements in 6dpl:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C also contains other interesting chemical elements:

Rubidium (Rb) 6 atoms
Chlorine (Cl) 3 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C (pdb code 6dpl). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C, PDB code: 6dpl:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dpl

Go back to Magnesium Binding Sites List in 6dpl
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:19.5
occ:1.00
OP1 b:C5 1.9 38.0 0.6
O A:HOH326 2.0 19.3 1.0
OD2 A:ASP71 2.0 20.4 1.0
OD1 A:ASP192 2.0 25.8 1.0
O b:HOH101 2.1 26.6 1.0
O A:HOH317 2.1 21.4 1.0
OP1 b:C5 2.2 18.0 0.5
CG A:ASP71 3.1 19.5 1.0
P b:C5 3.1 45.2 0.6
CG A:ASP192 3.1 25.2 1.0
HA A:ASP192 3.3 25.9 1.0
P b:C5 3.3 16.9 0.5
OD1 A:ASP71 3.4 19.6 1.0
OP2 b:C5 3.5 37.4 0.6
H A:TYR193 3.6 27.4 1.0
OP2 b:C5 3.7 17.3 0.5
HB2 A:ASP192 3.7 29.2 1.0
CB A:ASP192 3.8 24.4 1.0
MG A:MG202 3.8 18.1 1.0
O5' b:C5 3.9 36.5 0.6
RB A:RB203 4.0 28.1 0.2
CA A:ASP192 4.0 21.6 1.0
OP2 b:G6 4.0 20.0 1.0
OE1 A:GLU188 4.1 35.7 1.0
O5' b:C5 4.1 16.4 0.5
O A:VAL72 4.1 20.1 1.0
OD2 A:ASP192 4.1 30.1 1.0
OD2 A:ASP132 4.2 23.8 1.0
HG22 A:ILE189 4.2 22.1 1.0
N A:TYR193 4.3 22.8 1.0
O3' B:U4 4.3 43.0 0.6
RB A:RB205 4.3 39.0 0.2
CB A:ASP71 4.4 17.2 1.0
OP3 b:C5 4.4 16.5 0.5
C5' b:C5 4.4 23.8 1.0
HB3 A:ASP71 4.5 20.7 1.0
HB2 A:ASP71 4.7 20.7 1.0
HD1 A:TYR193 4.7 28.8 1.0
HG21 A:ILE189 4.7 22.1 1.0
HB3 A:ASP192 4.8 29.2 1.0
C A:ASP192 4.8 22.7 1.0
CG2 A:ILE189 4.8 18.4 1.0
CG A:GLU188 4.9 64.7 1.0
CD A:GLU188 4.9 78.9 1.0
HB2 A:TYR193 4.9 30.8 1.0
HG23 A:ILE189 5.0 22.1 1.0

Magnesium binding site 2 out of 2 in 6dpl

Go back to Magnesium Binding Sites List in 6dpl
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 720 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:18.1
occ:1.00
OD1 A:ASP71 2.0 19.6 1.0
OD1 A:ASP132 2.1 20.7 1.0
OE1 A:GLU109 2.1 20.5 1.0
OP1 b:C5 2.2 18.0 0.5
OP1 b:C5 2.4 38.0 0.6
OP3 b:C5 2.5 16.5 0.5
O3' B:U4 2.5 43.0 0.6
CG A:ASP132 2.9 20.4 1.0
P b:C5 2.9 16.9 0.5
P b:C5 3.0 45.2 0.6
CD A:GLU109 3.0 29.6 1.0
OD2 A:ASP132 3.0 23.8 1.0
CG A:ASP71 3.1 19.5 1.0
OE2 A:GLU109 3.3 23.4 1.0
O A:HOH334 3.4 21.9 1.0
HG12 A:VAL72 3.6 21.2 1.0
OD2 A:ASP71 3.6 20.4 1.0
H A:VAL72 3.7 19.0 1.0
C3' B:U4 3.7 30.9 0.6
MG A:MG201 3.8 19.5 1.0
O A:HOH326 3.9 19.3 1.0
HA A:ASP71 3.9 16.8 1.0
C5' b:C5 4.0 23.8 1.0
O5' b:C5 4.0 16.4 0.5
O5' b:C5 4.0 36.5 0.6
C4' B:U4 4.1 16.1 0.6
OP2 b:C5 4.1 17.3 0.5
HB2 A:SER133 4.2 20.1 1.0
OP2 b:C5 4.2 37.4 0.6
O2' B:U4 4.2 15.6 0.6
H A:ASP132 4.3 17.6 1.0
CB A:ASP132 4.3 18.8 1.0
CB A:ASP71 4.4 17.2 1.0
CG A:GLU109 4.4 15.2 1.0
C5' B:U4 4.5 16.9 0.6
N A:VAL72 4.5 15.8 1.0
CG1 A:VAL72 4.5 17.7 1.0
HG21 A:ILE189 4.5 22.1 1.0
HG3 A:GLU109 4.6 18.2 1.0
C2' B:U4 4.6 17.9 0.6
CA A:ASP71 4.6 14.0 1.0
HB2 A:ASP71 4.6 20.7 1.0
HG2 A:GLU109 4.7 18.2 1.0
O A:HOH317 4.8 21.4 1.0
HG13 A:VAL72 4.8 21.2 1.0
C A:ASP132 4.8 17.6 1.0
HB2 A:ASP132 4.8 22.6 1.0
HB3 A:ASP132 4.8 22.6 1.0
O A:VAL72 4.9 20.1 1.0
CA A:ASP132 4.9 16.0 1.0
HG11 A:VAL72 4.9 21.2 1.0
N A:ASP132 4.9 14.6 1.0
N A:SER133 5.0 15.4 1.0
O b:HOH101 5.0 26.6 1.0
RB A:RB204 5.0 22.5 0.5

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Dec 14 22:36:24 2020

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