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Magnesium in PDB 6dpm: Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C, PDB code: 6dpm was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.09 / 1.68
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.843, 37.454, 62.728, 90.00, 97.69, 90.00
R / Rfree (%) 16 / 19.9

Other elements in 6dpm:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C also contains other interesting chemical elements:

Rubidium (Rb) 6 atoms
Chlorine (Cl) 3 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C (pdb code 6dpm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C, PDB code: 6dpm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 6dpm

Go back to Magnesium Binding Sites List in 6dpm
Magnesium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg201

b:20.5
occ:1.00
OD2 A:ASP71 2.1 17.5 1.0
OD1 A:ASP192 2.1 21.2 1.0
O A:HOH314 2.1 16.9 1.0
O A:HOH318 2.1 19.7 1.0
OP1 b:C5 2.2 20.9 1.0
O b:HOH105 2.2 20.1 1.0
CG A:ASP71 3.1 20.3 1.0
CG A:ASP192 3.1 24.9 1.0
HA A:ASP192 3.3 23.3 1.0
P b:C5 3.3 21.6 1.0
OD1 A:ASP71 3.5 18.1 1.0
H A:TYR193 3.6 20.9 1.0
HB2 A:ASP192 3.6 26.9 1.0
OP2 b:C5 3.7 20.5 1.0
MG A:MG202 3.8 16.7 1.0
CB A:ASP192 3.8 22.4 1.0
RB A:RB203 3.9 25.9 0.2
OP2 b:G6 3.9 21.2 1.0
CA A:ASP192 4.0 19.4 1.0
O5' b:C5 4.0 21.2 1.0
O A:VAL72 4.1 17.8 1.0
OD2 A:ASP192 4.1 24.4 1.0
OD2 A:ASP132 4.1 22.3 1.0
OE1 A:GLU188 4.1 24.7 1.0
HG22 A:ILE189 4.2 22.6 1.0
N A:TYR193 4.3 17.5 1.0
RB A:RB205 4.4 37.0 0.2
CB A:ASP71 4.4 18.3 1.0
C5' b:C5 4.4 23.2 1.0
OP3 b:C5 4.4 22.7 1.0
HB3 A:ASP71 4.5 22.0 1.0
HB3 A:ASP192 4.7 26.9 1.0
HD1 A:TYR193 4.7 25.8 1.0
HG21 A:ILE189 4.7 22.6 1.0
C A:ASP192 4.7 19.8 1.0
HB2 A:ASP71 4.8 22.0 1.0
CG2 A:ILE189 4.8 18.8 1.0
HG23 A:ILE189 4.9 22.6 1.0
CG A:ASP132 5.0 16.0 1.0

Magnesium binding site 2 out of 2 in 6dpm

Go back to Magnesium Binding Sites List in 6dpm
Magnesium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 10 Mm MG2+ and 200 Mm Rb+ For 1800 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg202

b:16.7
occ:1.00
OD1 A:ASP132 2.0 16.4 1.0
OD1 A:ASP71 2.0 18.1 1.0
OE1 A:GLU109 2.0 16.8 1.0
OP1 b:C5 2.1 20.9 1.0
OP3 b:C5 2.3 22.7 1.0
P b:C5 2.7 21.6 1.0
CG A:ASP132 2.9 16.0 1.0
CD A:GLU109 3.1 16.8 1.0
CG A:ASP71 3.1 20.3 1.0
OD2 A:ASP132 3.1 22.3 1.0
OE2 A:GLU109 3.4 19.1 1.0
OD2 A:ASP71 3.5 17.5 1.0
HG12 A:VAL72 3.5 21.9 1.0
O A:HOH342 3.7 17.4 1.0
H A:VAL72 3.8 20.5 1.0
MG A:MG201 3.8 20.5 1.0
O5' b:C5 3.8 21.2 1.0
C5' b:C5 3.8 23.2 1.0
OP2 b:C5 3.9 20.5 1.0
O A:HOH314 4.0 16.9 1.0
HA A:ASP71 4.1 16.7 1.0
HB2 A:SER133 4.1 19.3 1.0
O b:HOH102 4.2 21.6 1.0
H A:ASP132 4.3 16.9 1.0
O b:HOH103 4.3 20.2 1.0
CB A:ASP132 4.4 16.7 1.0
CG A:GLU109 4.4 13.2 1.0
CB A:ASP71 4.4 18.3 1.0
HG3 A:GLU109 4.5 15.8 1.0
CG1 A:VAL72 4.5 18.2 1.0
N A:VAL72 4.5 17.1 1.0
HG21 A:ILE189 4.6 22.6 1.0
HB2 A:ASP71 4.7 22.0 1.0
C A:ASP132 4.7 19.1 1.0
HG2 A:GLU109 4.7 15.8 1.0
CA A:ASP71 4.7 13.9 1.0
O A:HOH318 4.8 19.7 1.0
HG13 A:VAL72 4.8 21.9 1.0
HB2 A:ASP132 4.8 20.0 1.0
HG11 A:VAL72 4.9 21.9 1.0
HB3 A:ASP132 4.9 20.0 1.0
O A:VAL72 4.9 17.8 1.0
CA A:ASP132 4.9 13.9 1.0
N A:SER133 4.9 15.1 1.0
O A:ASP132 4.9 20.4 1.0
O b:HOH105 4.9 20.1 1.0
N A:ASP132 4.9 14.1 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Sep 30 23:25:15 2024

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