Magnesium in PDB 6eg8: Structure of the Gdp-Bound Gs Heterotrimer

The structure of Structure of the Gdp-Bound Gs Heterotrimer, PDB code: 6eg8 was solved by D.Hilger, X.Liu, P.Aschauer, B.K.Kobilka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.43 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	100.860, 100.810, 179.330, 90.00, 106.18, 90.00
R / Rfree (%)	21.7 / 26.9

The binding sites of Magnesium atom in the Structure of the Gdp-Bound Gs Heterotrimer (pdb code 6eg8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of the Gdp-Bound Gs Heterotrimer, PDB code: 6eg8:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Structure of the Gdp-Bound Gs Heterotrimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Gdp-Bound Gs Heterotrimer within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg402 b:22.3 occ:1.00 O I:HOH504 2.1 24.2 1.0 O3B I:GDP401 2.2 33.2 1.0 OG I:SER54 2.4 23.3 1.0 O I:HOH503 2.5 27.0 1.0 OG1 I:THR204 2.7 42.8 1.0 PB I:GDP401 3.4 32.0 1.0 CB I:THR204 3.4 43.6 1.0 O1B I:GDP401 3.4 31.2 1.0 CB I:SER54 3.5 24.4 1.0 O2A I:GDP401 3.7 19.3 1.0 OD2 I:ASP223 3.9 52.5 1.0 N I:THR204 3.9 43.7 1.0 NH1 I:ARG201 4.1 25.9 1.0 O I:VAL202 4.2 47.1 1.0 CA I:THR204 4.3 43.2 1.0 O2B I:GDP401 4.4 31.6 1.0 N I:SER54 4.4 30.4 1.0 O3A I:GDP401 4.5 30.4 1.0 PA I:GDP401 4.5 23.6 1.0 CG2 I:THR204 4.5 44.1 1.0 CA I:SER54 4.6 26.5 1.0 O1A I:GDP401 4.7 22.5 1.0 C I:LEU203 5.0 46.5 1.0 CZ I:ARG201 5.0 24.1 1.0

Magnesium binding site 2 out of 4 in the Structure of the Gdp-Bound Gs Heterotrimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Gdp-Bound Gs Heterotrimer within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg402 b:29.6 occ:1.00 O J:HOH507 2.1 30.1 1.0 O3B J:GDP401 2.1 28.7 1.0 OG J:SER54 2.2 29.4 1.0 O J:HOH515 2.2 40.6 1.0 O J:HOH513 2.3 27.7 1.0 OG1 J:THR204 2.4 33.2 1.0 CB J:THR204 3.1 33.7 1.0 PB J:GDP401 3.5 28.7 1.0 CB J:SER54 3.5 30.8 1.0 O2B J:GDP401 3.8 29.2 1.0 N J:THR204 3.8 34.8 1.0 OD2 J:ASP223 3.9 46.4 1.0 CA J:THR204 4.1 34.8 1.0 O2A J:GDP401 4.1 24.8 1.0 O1B J:GDP401 4.2 29.2 1.0 N J:SER54 4.3 33.0 1.0 CG2 J:THR204 4.3 33.9 1.0 OD1 J:ASP223 4.3 47.2 1.0 O J:VAL202 4.4 45.6 1.0 CA J:SER54 4.4 32.9 1.0 CG J:ASP223 4.5 44.9 1.0 O3A J:GDP401 4.6 28.6 1.0 NH1 J:ARG201 4.6 37.0 1.0 PA J:GDP401 4.8 25.8 1.0 O1A J:GDP401 4.9 26.1 1.0 NZ J:LYS53 4.9 37.8 1.0 C J:LEU203 5.0 37.0 1.0 CE J:LYS53 5.0 37.7 1.0

Magnesium binding site 3 out of 4 in the Structure of the Gdp-Bound Gs Heterotrimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of the Gdp-Bound Gs Heterotrimer within 5.0Å range: probe atom residue distance (Å) B Occ K:Mg402 b:26.2 occ:1.00 OG K:SER54 2.0 25.9 1.0 O1B K:GDP401 2.3 25.7 1.0 OG1 K:THR204 2.5 44.0 1.0 O K:HOH505 2.6 19.5 1.0 CB K:SER54 2.8 25.2 1.0 O K:HOH503 3.0 24.3 1.0 O2A K:GDP401 3.1 22.9 1.0 O K:VAL202 3.3 41.3 1.0 NH1 K:ARG201 3.5 28.1 1.0 PB K:GDP401 3.6 27.0 1.0 CB K:THR204 3.8 46.0 1.0 N K:THR204 3.9 42.1 1.0 PA K:GDP401 4.0 22.3 1.0 CA K:SER54 4.1 26.0 1.0 O3A K:GDP401 4.1 25.8 1.0 O1A K:GDP401 4.2 22.1 1.0 N K:SER54 4.3 25.9 1.0 O2B K:GDP401 4.3 29.7 1.0 CA K:THR204 4.4 45.7 1.0 C K:VAL202 4.4 38.2 1.0 C K:LEU203 4.6 41.5 1.0 OD2 K:ASP223 4.6 50.6 1.0 CA K:LEU203 4.6 42.3 1.0 O3B K:GDP401 4.7 27.7 1.0 CZ K:ARG201 4.8 25.4 1.0 CG2 K:THR204 4.9 46.5 1.0

Magnesium binding site 4 out of 4 in the Structure of the Gdp-Bound Gs Heterotrimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of the Gdp-Bound Gs Heterotrimer within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg402 b:19.6 occ:1.00 O3B L:GDP401 2.0 19.2 1.0 OG L:SER54 2.1 22.8 1.0 O L:HOH508 2.1 20.8 1.0 O L:HOH507 2.4 19.1 1.0 OG1 L:THR204 2.4 33.3 1.0 CB L:THR204 3.2 33.9 1.0 CB L:SER54 3.2 26.4 1.0 PB L:GDP401 3.3 19.2 1.0 O1B L:GDP401 3.4 21.4 1.0 O1A L:GDP401 3.7 19.3 1.0 O L:HOH510 3.9 45.9 1.0 N L:THR204 3.9 33.0 1.0 NH1 L:ARG201 3.9 33.8 1.0 N L:SER54 4.0 30.3 1.0 OD2 L:ASP223 4.1 51.4 1.0 O L:VAL202 4.2 39.8 1.0 CA L:THR204 4.2 34.6 1.0 CA L:SER54 4.2 29.0 1.0 O2B L:GDP401 4.2 20.2 1.0 O3A L:GDP401 4.3 21.4 1.0 CG2 L:THR204 4.3 34.1 1.0 PA L:GDP401 4.4 19.3 1.0 O2A L:GDP401 4.6 19.4 1.0 C L:LEU203 5.0 34.7 1.0

X.Liu, X.Xu, D.Hilger, P.Aschauer, J.K.S.Tiemann, Y.Du, H.Liu, K.Hirata, X.Sun, R.Guixa-Gonzalez, J.M.Mathiesen, P.W.Hildebrand, B.K.Kobilka. Structural Insights Into the Process of Gpcr-G Protein Complex Formation. Cell V. 177 1243 2019.
ISSN: ISSN 1097-4172
PubMed: 31080070
DOI: 10.1016/J.CELL.2019.04.021