Magnesium in PDB 6ff8: Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution

The structure of Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution, PDB code: 6ff8 was solved by A.R.Khan, T.Kecman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.35 / 2.13
Space group	P 43
Cell size a, b, c (Å), α, β, γ (°)	53.658, 53.658, 129.784, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 22.3

The binding sites of Magnesium atom in the Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution (pdb code 6ff8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution, PDB code: 6ff8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg901 b:25.2 occ:1.00 O1G A:GTP900 2.0 27.0 1.0 O1B A:GTP900 2.0 25.0 1.0 OG1 A:THR39 2.2 30.4 1.0 O A:HOH1003 2.2 26.3 1.0 OG1 A:THR57 2.2 36.2 1.0 O A:HOH1001 2.2 29.3 1.0 CB A:THR39 3.1 25.7 1.0 PB A:GTP900 3.2 26.2 1.0 PG A:GTP900 3.2 28.2 1.0 CB A:THR57 3.2 31.6 1.0 O3B A:GTP900 3.4 27.2 1.0 N A:THR57 3.8 26.2 1.0 O2G A:GTP900 3.9 29.9 1.0 O2A A:GTP900 3.9 25.6 1.0 OD2 A:ASP81 4.0 27.6 1.0 N A:THR39 4.0 21.6 1.0 O3A A:GTP900 4.1 25.4 1.0 CG2 A:THR39 4.1 23.7 1.0 CA A:THR39 4.1 23.0 1.0 CA A:THR57 4.1 28.7 1.0 OD1 A:ASP81 4.3 27.8 1.0 O3G A:GTP900 4.3 27.8 1.0 O2B A:GTP900 4.4 26.5 1.0 PA A:GTP900 4.4 26.3 1.0 O A:ARG55 4.4 23.2 1.0 CG2 A:THR57 4.4 29.9 1.0 CG A:ASP81 4.6 26.6 1.0 O1A A:GTP900 4.6 29.1 1.0 C A:ALA56 4.8 25.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Uncomplexed RAB32 in the Active Gtp-Bound State at 2.13 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg901 b:36.8 occ:1.00 O3G B:GTP900 1.8 35.0 1.0 O B:HOH1008 2.2 37.0 1.0 OG1 B:THR39 2.2 23.2 1.0 O2B B:GTP900 2.2 25.8 1.0 O B:HOH1002 2.3 46.0 1.0 OG1 B:THR57 2.4 32.1 1.0 PG B:GTP900 3.0 34.0 1.0 PB B:GTP900 3.1 28.8 1.0 CB B:THR39 3.1 24.4 1.0 CB B:THR57 3.2 29.5 1.0 O3B B:GTP900 3.2 30.6 1.0 N B:THR57 3.7 31.4 1.0 O2G B:GTP900 3.8 32.8 1.0 O1A B:GTP900 3.9 29.9 1.0 OD2 B:ASP81 4.0 34.6 1.0 N B:THR39 4.0 25.1 1.0 CA B:THR57 4.0 31.4 1.0 CG2 B:THR39 4.1 24.1 1.0 O3A B:GTP900 4.2 27.4 1.0 CA B:THR39 4.2 23.8 1.0 OD1 B:ASP81 4.2 36.6 1.0 O1G B:GTP900 4.3 37.0 1.0 O1B B:GTP900 4.3 28.0 1.0 CG2 B:THR57 4.4 25.3 1.0 PA B:GTP900 4.4 28.2 1.0 O B:ARG55 4.5 34.4 1.0 CG B:ASP81 4.5 32.9 1.0 C B:ALA56 4.7 33.0 1.0 O2A B:GTP900 4.8 27.7 1.0

E.Mcgrath, D.Waschbusch, B.M.Baker, A.R.Khan. LRRK2 Binds to the RAB32 Subfamily in A Gtp-Dependent Mannerviaits Armadillo Domain. Small Gtpases 1 2019.
ISSN: ISSN 2154-1256
PubMed: 31552791
DOI: 10.1080/21541248.2019.1666623