Magnesium in PDB 6flp: Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa

Enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa:
2.7.7.6;

Other elements in 6flp:

The structure of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa (pdb code 6flp). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa, PDB code: 6flp:

Magnesium binding site 1 out of 1 in 6flp

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Magnesium Binding Sites List in 6flp

Magnesium binding site 1 out of 1 in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
D:Mg1501 b:0.8 occ:1.00	OD2	D:ASP462	2.6	0.9	1.0
	OD1	D:ASP464	3.0	0.4	1.0
	OD1	D:ASP462	3.0	0.9	1.0
	OD2	D:ASP464	3.1	0.4	1.0
	C5'	R:U29	3.1	0.6	1.0
	CG	D:ASP462	3.2	0.9	1.0
	CG	D:ASP464	3.4	0.4	1.0
	OP1	R:U29	3.7	0.6	1.0
	C3'	R:U29	3.8	0.6	1.0
	C4'	R:U29	3.9	0.6	1.0
	O5'	R:U29	4.2	0.6	1.0
	O3'	R:U29	4.6	0.6	1.0
	P	R:U29	4.7	0.6	1.0
	CB	D:ASP462	4.7	0.9	1.0
	NH2	D:ARG425	4.8	0.9	1.0
	C2'	R:U29	4.9	0.6	1.0
	CB	D:ASP464	4.9	0.4	1.0
	N	D:ASP460	5.0	0.3	1.0

Reference:

X.Guo, A.G.Myasnikov, J.Chen, C.Crucifix, G.Papai, M.Takacs, P.Schultz, A.Weixlbaumer. Structural Basis For Nusa Stabilized Transcriptional Pausing. Mol. Cell V. 69 816 2018.
ISSN: ISSN 1097-4164
PubMed: 29499136
DOI: 10.1016/J.MOLCEL.2018.02.008

Page generated: Wed Aug 13 06:09:12 2025

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