Magnesium in PDB 6gmo: Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm)

All present enzymatic activity of Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm):
6.3.2.2;

The structure of Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm), PDB code: 6gmo was solved by E.D.Lenherr, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.72 / 1.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	58.770, 109.860, 84.750, 90.00, 98.72, 90.00
R / Rfree (%)	16.3 / 19.8

The binding sites of Magnesium atom in the Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm) (pdb code 6gmo). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm), PDB code: 6gmo:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:18.2 occ:1.00 OE1 A:GLU165 2.2 27.3 1.0 OE2 A:GLU107 2.2 16.2 1.0 O A:HOH999 2.2 20.5 1.0 OE2 A:GLU159 2.3 24.9 1.0 O A:HOH937 2.3 30.4 1.0 O A:HOH1042 2.3 33.7 1.0 H1 A:HOH1042 2.5 35.8 0.0 H2 A:HOH1042 2.6 35.8 0.0 H1 A:HOH999 2.8 20.9 0.0 HG11 A:GSM602 3.0 30.7 0.0 H1 A:HOH937 3.0 33.4 0.0 H2 A:HOH999 3.0 20.9 0.0 H2 A:HOH937 3.1 33.4 0.0 CD A:GLU165 3.2 31.0 1.0 CD A:GLU159 3.2 24.1 1.0 CD A:GLU107 3.2 16.9 1.0 H2 A:HOH912 3.5 28.6 0.0 HG2 A:GLU107 3.6 12.1 0.0 HG3 A:GLU107 3.6 12.1 0.0 HN3 A:GSM602 3.7 34.5 0.0 CG A:GLU107 3.7 10.3 1.0 OE2 A:GLU165 3.8 22.9 1.0 HG2 A:GLU159 3.8 12.4 0.0 HG3 A:GLU159 3.8 12.4 0.0 CG A:GLU159 3.8 10.5 1.0 CG1 A:GSM602 4.0 30.5 1.0 OE1 A:GLU159 4.0 28.6 1.0 HB2 A:GLU165 4.1 12.7 0.0 H1 A:HOH912 4.1 28.6 0.0 O A:HOH912 4.1 26.3 1.0 O A:HOH726 4.2 23.3 1.0 H1 A:HOH832 4.2 27.8 0.0 CG A:GLU165 4.3 16.2 1.0 HB12 A:GSM602 4.3 28.1 0.0 OE1 A:GLU107 4.3 13.8 1.0 HG3 A:GLU165 4.3 16.4 0.0 H1 A:HOH726 4.4 24.6 0.0 N3 A:GSM602 4.4 34.3 1.0 HG12 A:GSM602 4.4 30.7 0.0 CD1 A:GSM602 4.4 34.0 1.0 HN2 A:GSM602 4.4 26.9 0.0 H1 A:HOH955 4.4 34.5 0.0 H2 A:HOH955 4.4 34.5 0.0 O32 A:GSM602 4.5 70.3 1.0 HG22 A:THR244 4.5 16.0 0.0 H2 A:HOH726 4.6 24.6 0.0 OE1 A:GLU105 4.6 22.2 1.0 N2 A:GSM602 4.6 24.0 1.0 CB A:GLU165 4.6 11.2 1.0 HA31 A:GSM602 4.6 20.4 0.0 CB1 A:GSM602 4.7 29.6 1.0 O A:HOH832 4.8 28.3 1.0 HB3 A:GLU165 4.8 12.7 0.0 O A:HOH955 4.9 32.1 1.0 HG21 A:THR244 4.9 16.0 0.0 CA3 A:GSM602 4.9 44.8 1.0

Magnesium binding site 2 out of 2 in the Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Plant Glutamate Cysteine Ligase (Gcl) in Complex with Non-Reducing Gsh (Gsm) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:29.9 occ:1.00 OE2 B:GLU107 2.2 29.8 1.0 OE1 B:GLU165 2.3 24.2 0.5 O B:HOH932 2.3 31.2 1.0 OE2 B:GLU159 2.5 26.5 1.0 HG11 B:GSM602 2.6 34.6 0.0 HG3 B:GLU165 2.9 14.9 0.0 H1 B:HOH932 3.0 31.6 0.0 H2 B:HOH744 3.0 26.4 0.0 H2 B:HOH932 3.1 31.6 0.0 CD B:GLU165 3.2 23.2 0.5 CD B:GLU107 3.2 19.0 1.0 HG2 B:GLU107 3.4 18.2 0.0 CG1 B:GSM602 3.4 38.7 1.0 CD B:GLU159 3.5 25.4 1.0 HG12 B:GSM602 3.5 34.6 0.0 H1 B:HOH996 3.6 32.1 0.0 CG B:GLU107 3.7 17.0 1.0 HG3 B:GLU107 3.7 18.2 0.0 OE2 B:GLU165 3.7 21.5 0.5 HN3 B:GSM602 3.8 32.0 0.0 CG B:GLU165 3.8 13.1 0.5 O B:HOH744 3.9 30.5 1.0 HG3 B:GLU159 4.0 14.7 0.0 HN2 B:GSM602 4.1 36.2 0.0 OE1 B:GLU165 4.1 19.0 0.5 HB12 B:GSM602 4.1 33.3 0.0 HG2 B:GLU159 4.1 14.7 0.0 HG3 B:GLU165 4.1 14.9 0.0 CG B:GLU159 4.1 12.9 1.0 CG B:GLU165 4.2 13.2 0.5 HB2 B:GLU165 4.2 13.7 0.0 HG2 B:GLU165 4.2 14.9 0.0 CD1 B:GSM602 4.2 42.9 1.0 OE1 B:GLU107 4.3 14.9 1.0 OE1 B:GLU159 4.3 20.9 1.0 CD B:GLU165 4.3 17.6 0.5 O B:HOH778 4.4 35.0 1.0 HG22 B:THR244 4.4 21.3 0.0 CB1 B:GSM602 4.4 34.0 1.0 H1 B:HOH778 4.4 32.7 0.0 N2 B:GSM602 4.4 37.5 1.0 OE1 B:GLU105 4.4 15.7 1.0 HB2 B:GLU165 4.4 13.7 0.0 H1 B:HOH744 4.5 26.4 0.0 O B:HOH996 4.5 33.4 1.0 HG21 B:THR244 4.6 21.3 0.0 N3 B:GSM602 4.7 29.7 1.0 CB B:GLU165 4.7 12.2 0.5 CB B:GLU165 4.7 12.2 0.5 H2 B:HOH996 4.8 32.1 0.0 CG2 B:THR244 4.8 20.1 1.0 HG23 B:THR244 4.8 21.3 0.0 HB3 B:GLU105 4.9 12.7 0.0 HB11 B:GSM602 4.9 33.3 0.0 HB3 B:GLU165 5.0 13.7 0.0

Y.Yang, E.Lenherr, R.Gromes, S.Wang, M.Wirtz, R.Hell, K.Scheffzek, T.Rausch. Regulation of Plant Glutamylcysteine Ligase: Redox-Mediated Enzyme Activation Does Not Require Homodimerization To Be Published.