Magnesium in PDB 6gwc: Tubulin:IE5 Alpharep Complex

The structure of Tubulin:IE5 Alpharep Complex, PDB code: 6gwc was solved by B.Gigant, V.Campanacci, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.25 / 2.60
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	102.346, 102.346, 216.207, 90.00, 90.00, 120.00
R / Rfree (%)	17.3 / 22.3

The binding sites of Magnesium atom in the Tubulin:IE5 Alpharep Complex (pdb code 6gwc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Tubulin:IE5 Alpharep Complex, PDB code: 6gwc:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Tubulin:IE5 Alpharep Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tubulin:IE5 Alpharep Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:44.1 occ:1.00 O A:HOH702 2.0 53.1 1.0 O A:HOH722 2.0 42.0 1.0 O A:HOH750 2.1 51.1 1.0 O3G A:GTP600 2.1 48.0 1.0 O A:HOH749 2.2 47.4 1.0 O2B A:GTP600 2.2 39.1 1.0 PG A:GTP600 3.2 48.6 1.0 PB A:GTP600 3.4 43.8 1.0 O3B A:GTP600 3.6 46.6 1.0 O2G A:GTP600 3.6 50.3 1.0 NZ B:LYS254 3.8 65.1 1.0 OE2 A:GLU71 3.9 62.8 1.0 O3A A:GTP600 4.0 45.8 1.0 OD2 A:ASP69 4.1 55.1 1.0 CB A:GLN11 4.2 46.4 1.0 OD1 A:ASP69 4.3 55.9 1.0 CB A:ASP98 4.5 49.4 1.0 O1G A:GTP600 4.5 46.5 1.0 N A:GLN11 4.5 45.7 1.0 O2A A:GTP600 4.6 46.7 1.0 O1B A:GTP600 4.6 40.1 1.0 CG A:ASP69 4.6 55.6 1.0 OD2 A:ASP98 4.7 76.8 1.0 CG A:ASP98 4.7 61.9 1.0 OE1 A:GLN11 4.7 56.0 1.0 PA A:GTP600 4.9 46.0 1.0 CD A:GLU71 5.0 79.1 1.0 CA A:GLN11 5.0 44.9 1.0 CE B:LYS254 5.0 53.8 1.0

Magnesium binding site 2 out of 2 in the Tubulin:IE5 Alpharep Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Tubulin:IE5 Alpharep Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:86.1 occ:1.00 O B:HOH704 2.1 58.1 1.0 O3G B:GTP600 2.7 88.1 1.0 O B:HOH703 2.8 54.9 1.0 O2B B:GTP600 3.1 72.5 1.0 NE2 B:GLN11 3.3 0.5 1.0 CB B:GLN11 3.5 67.3 1.0 O2A B:GTP600 3.6 68.1 1.0 PG B:GTP600 3.9 84.9 1.0 PB B:GTP600 4.0 72.2 1.0 O3A B:GTP600 4.0 70.6 1.0 CD B:GLN11 4.1 0.0 1.0 O2G B:GTP600 4.2 85.4 1.0 CG B:GLN11 4.3 82.4 1.0 O3B B:GTP600 4.4 77.3 1.0 PA B:GTP600 4.4 64.6 1.0 N B:GLN11 4.5 63.8 1.0 CA B:GLN11 4.6 64.7 1.0 OD2 B:ASP69 4.9 85.0 1.0 O1A B:GTP600 5.0 62.9 1.0

V.Campanacci, A.Urvoas, T.Consolati, S.Cantos-Fernandes, M.Aumont-Nicaise, M.Valerio-Lepiniec, T.Surrey, P.Minard, B.Gigant. Selection and Characterization of Artificial Proteins Targeting the Tubulin Alpha Subunit. Structure V. 27 497 2019.
ISSN: ISSN 1878-4186
PubMed: 30661854
DOI: 10.1016/J.STR.2018.12.001