Magnesium in PDB 6jgj: Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A

The structure of Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A, PDB code: 6jgj was solved by K.Takaba, Y.Tai, Y.Hanazono, K.Miki, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.14 / 0.78
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.870, 62.340, 68.850, 90.00, 90.00, 90.00
R / Rfree (%)	10.8 / 12.5

The binding sites of Magnesium atom in the Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A (pdb code 6jgj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A, PDB code: 6jgj:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:22.8 occ:0.30 O A:HOH644 1.8 28.7 0.4 O A:HOH898 2.1 27.1 0.6 OD2 A:ASP197 2.5 15.9 1.0 O A:HOH898 2.7 33.1 0.4 OD1 A:ASP197 3.0 18.3 1.0 NH1 A:ARG80 3.1 20.8 1.0 CG A:ASP197 3.1 12.2 1.0 NH2 A:ARG80 3.6 17.9 1.0 CZ A:ARG80 3.8 13.8 1.0 HE1 A:HIS81 3.8 11.7 1.0 O A:HOH403 4.1 23.1 0.2 O A:HOH477 4.3 27.2 0.8 O A:HOH601 4.4 10.1 0.4 CB A:ASP197 4.6 9.9 1.0 O A:HOH555 4.6 31.6 1.0 CE1 A:HIS81 4.8 9.8 1.0 HB2 A:ASP197 4.9 11.8 1.0 O A:HOH427 4.9 21.1 0.5 O A:HOH1015 4.9 32.6 0.4

Magnesium binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the F99S/M153T/V163A/E222Q Variant of Gfp at 0.78 A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:7.3 occ:0.53 O A:HOH740 2.0 7.7 0.6 O A:HOH523 2.1 18.3 0.8 O A:HOH879 2.2 8.9 0.6 O A:HOH553 3.4 22.4 0.2 O A:HOH693 3.6 19.5 0.4 O A:HOH989 4.0 20.5 0.7 O A:HOH843 4.1 23.8 0.5 OG A:SER30 4.1 8.2 1.0 O A:HOH478 4.2 11.6 0.9 HB3 A:SER30 4.3 8.5 1.0 O A:HOH855 4.5 18.0 0.4 OE2 A:GLU17 4.6 8.2 0.6 O A:HOH693 4.6 41.3 0.6 CB A:SER30 4.7 7.1 1.0 OE2 A:GLU17 4.7 8.6 0.4 HB2 A:SER30 4.8 8.5 1.0 HG21 A:THR49 4.9 13.9 1.0

K.Takaba, Y.Tai, H.Eki, H.A.Dao, Y.Hanazono, K.Hasegawa, K.Miki, K.Takeda. Subatomic Resolution X-Ray Structures of Green Fluorescent Protein. Iucrj V. 6 387 2019.
ISSN: ESSN 2052-2525
PubMed: 31098020
DOI: 10.1107/S205225251900246X