Magnesium in PDB 6jt9: Crystal Structure of D464A Mutant of Fgam Synthetase

All present enzymatic activity of Crystal Structure of D464A Mutant of Fgam Synthetase:
6.3.5.3;

The structure of Crystal Structure of D464A Mutant of Fgam Synthetase, PDB code: 6jt9 was solved by N.Sharma, N.Ahalawat, P.Sandhu, J.Mondal, R.Anand, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.52 / 2.10
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	146.461, 146.461, 141.540, 90.00, 90.00, 120.00
R / Rfree (%)	14.5 / 18.9

The structure of Crystal Structure of D464A Mutant of Fgam Synthetase also contains other interesting chemical elements:

Chlorine

(Cl)

3 atoms

The binding sites of Magnesium atom in the Crystal Structure of D464A Mutant of Fgam Synthetase (pdb code 6jt9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of D464A Mutant of Fgam Synthetase, PDB code: 6jt9:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of D464A Mutant of Fgam Synthetase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of D464A Mutant of Fgam Synthetase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1301 b:13.3 occ:1.00 O3B A:ADP1316 2.0 14.5 1.0 O A:HOH1598 2.0 14.3 1.0 OD1 A:ASP884 2.0 15.9 1.0 OD1 A:ASP679 2.1 12.7 1.0 OD1 A:ASN722 2.1 14.2 1.0 O A:HOH1610 2.2 11.4 1.0 CG A:ASP884 3.0 15.5 1.0 CG A:ASP679 3.1 14.2 1.0 CG A:ASN722 3.2 15.2 1.0 PB A:ADP1316 3.2 15.3 1.0 OD2 A:ASP884 3.3 15.6 1.0 ND2 A:ASN722 3.6 14.8 1.0 O2B A:ADP1316 3.7 16.1 1.0 O1B A:ADP1316 3.8 15.6 1.0 CB A:ASP679 3.8 13.6 1.0 OD2 A:ASP679 3.9 14.9 1.0 O A:HOH1722 3.9 12.0 1.0 OG A:SER886 4.1 17.3 1.0 O A:HOH1962 4.2 22.0 1.0 CA A:ASP679 4.3 13.4 1.0 NE2 A:HIS883 4.3 14.1 1.0 CD2 A:HIS883 4.4 12.7 1.0 CB A:ASP884 4.4 15.4 1.0 O A:ASP884 4.4 18.8 1.0 O A:HOH1508 4.4 17.3 1.0 O3A A:ADP1316 4.5 14.4 1.0 CB A:ASN722 4.5 14.9 1.0 OE2 A:GLU718 4.6 16.5 1.0 N A:ASP884 4.7 14.3 1.0 CA A:ASN722 4.7 15.1 1.0 C A:ASP884 4.8 18.1 1.0 CA A:ASP884 4.8 15.0 1.0 O A:ASP679 5.0 14.7 1.0 CB A:SER886 5.0 17.0 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of D464A Mutant of Fgam Synthetase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of D464A Mutant of Fgam Synthetase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1302 b:17.2 occ:1.00 OE2 A:GLU718 2.0 16.5 1.0 O A:HOH1655 2.1 18.8 1.0 O A:HOH1784 2.1 14.8 1.0 O2B A:ADP1316 2.1 16.1 1.0 O A:HOH1477 2.1 20.5 1.0 O A:HOH1696 2.2 14.1 1.0 CD A:GLU718 3.0 18.7 1.0 PB A:ADP1316 3.3 15.3 1.0 OE1 A:GLU718 3.4 17.7 1.0 O1B A:ADP1316 3.7 15.6 1.0 O A:HOH1610 3.9 11.4 1.0 O A:HOH1629 4.0 17.4 1.0 ND2 A:ASN722 4.1 14.8 1.0 O3A A:ADP1316 4.1 14.4 1.0 O A:HOH1523 4.2 24.4 1.0 O1A A:ADP1316 4.2 15.1 1.0 OE1 A:GLU699 4.3 25.9 1.0 CG A:GLU718 4.3 16.7 1.0 OD2 A:ASP887 4.4 20.8 1.0 O A:ILE697 4.4 17.0 1.0 OG A:SER886 4.5 17.3 1.0 O3B A:ADP1316 4.5 14.5 1.0 CB A:SER886 4.7 17.0 1.0 O A:GLU699 4.8 16.5 1.0 PA A:ADP1316 4.9 14.9 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of D464A Mutant of Fgam Synthetase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of D464A Mutant of Fgam Synthetase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1303 b:16.8 occ:1.00 O2A A:ADP1316 2.0 13.8 1.0 O1B A:ADP1316 2.0 15.6 1.0 O A:HOH1457 2.1 17.1 1.0 O A:HOH1508 2.1 17.3 1.0 O A:HOH1857 2.2 15.1 1.0 O A:HOH1745 2.2 14.3 1.0 PA A:ADP1316 3.1 14.9 1.0 PB A:ADP1316 3.3 15.3 1.0 O3A A:ADP1316 3.5 14.4 1.0 O1A A:ADP1316 3.9 15.1 1.0 O A:HOH1641 4.0 19.0 1.0 O A:HOH1696 4.0 14.1 1.0 OE1 A:GLU896 4.1 16.9 1.0 OE2 A:GLU896 4.1 14.9 1.0 O3B A:ADP1316 4.2 14.5 1.0 OG A:SER886 4.2 17.3 1.0 O A:HOH1598 4.2 14.3 1.0 O A:HOH1722 4.3 12.0 1.0 O5' A:ADP1316 4.4 15.2 1.0 O2B A:ADP1316 4.5 16.1 1.0 O A:THR645 4.6 16.4 1.0 O A:VAL646 4.6 16.0 1.0 O A:HOH2102 4.6 18.9 1.0 OD2 A:ASP887 4.6 20.8 1.0 CD A:GLU896 4.6 16.1 1.0 C5' A:ADP1316 4.7 16.9 1.0 O A:ASP887 4.7 17.5 1.0 CB A:ASP887 5.0 20.7 1.0

N.Sharma, N.Ahalawat, P.Sandhu, J.Mondal, R.Anand. Crystal Structure of D464A Mutant of Fgam Synthetase To Be Published.