Magnesium in PDB 6jup: Mutant Poliv-Dna Incoming Nucleotide Complex
Enzymatic activity of Mutant Poliv-Dna Incoming Nucleotide Complex
All present enzymatic activity of Mutant Poliv-Dna Incoming Nucleotide Complex:
2.7.7.7;
Protein crystallography data
The structure of Mutant Poliv-Dna Incoming Nucleotide Complex, PDB code: 6jup
was solved by
D.T.Nair,
M.K.Johnson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
69.71 /
2.44
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
86.184,
56.974,
111.136,
90.00,
92.90,
90.00
|
R / Rfree (%)
|
22.1 /
24.5
|
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Mutant Poliv-Dna Incoming Nucleotide Complex
(pdb code 6jup). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the
Mutant Poliv-Dna Incoming Nucleotide Complex, PDB code: 6jup:
Jump to Magnesium binding site number:
1;
2;
3;
4;
Magnesium binding site 1 out
of 4 in 6jup
Go back to
Magnesium Binding Sites List in 6jup
Magnesium binding site 1 out
of 4 in the Mutant Poliv-Dna Incoming Nucleotide Complex
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Mutant Poliv-Dna Incoming Nucleotide Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Mg402
b:58.6
occ:1.00
|
O2B
|
F:0KX401
|
1.7
|
97.2
|
1.0
|
OD1
|
F:ASP8
|
2.0
|
0.8
|
1.0
|
O
|
F:MET9
|
2.0
|
72.4
|
1.0
|
O1G
|
F:0KX401
|
2.3
|
0.5
|
1.0
|
O2A
|
F:0KX401
|
2.3
|
85.1
|
1.0
|
OD2
|
F:ASP103
|
2.3
|
81.4
|
1.0
|
PB
|
F:0KX401
|
3.1
|
68.7
|
1.0
|
CG
|
F:ASP8
|
3.1
|
81.2
|
1.0
|
C
|
F:MET9
|
3.2
|
76.2
|
1.0
|
MG
|
F:MG403
|
3.3
|
56.8
|
1.0
|
PA
|
F:0KX401
|
3.5
|
69.3
|
1.0
|
PG
|
F:0KX401
|
3.5
|
77.0
|
1.0
|
CG
|
F:ASP103
|
3.6
|
65.2
|
1.0
|
N3A
|
F:0KX401
|
3.7
|
75.6
|
1.0
|
O3B
|
F:0KX401
|
3.7
|
77.4
|
1.0
|
OD2
|
F:ASP8
|
3.8
|
89.2
|
1.0
|
C5'
|
F:0KX401
|
3.8
|
64.7
|
1.0
|
N
|
F:MET9
|
3.8
|
66.9
|
1.0
|
CA
|
F:MET9
|
4.0
|
81.1
|
1.0
|
O5'
|
F:0KX401
|
4.1
|
61.2
|
1.0
|
O3G
|
F:0KX401
|
4.1
|
80.7
|
1.0
|
O1B
|
F:0KX401
|
4.2
|
79.4
|
1.0
|
N
|
F:ASP10
|
4.2
|
81.0
|
1.0
|
C
|
F:ASP8
|
4.2
|
71.0
|
1.0
|
CB
|
F:ASP8
|
4.3
|
81.8
|
1.0
|
CA
|
F:ASP10
|
4.4
|
84.0
|
1.0
|
CB
|
F:ASP103
|
4.4
|
69.0
|
1.0
|
OD1
|
F:ASP103
|
4.4
|
0.2
|
1.0
|
NZ
|
F:LYS157
|
4.5
|
81.8
|
1.0
|
CB
|
F:MET9
|
4.5
|
78.9
|
1.0
|
N
|
F:CYS11
|
4.6
|
79.2
|
1.0
|
CA
|
F:ASP8
|
4.6
|
83.7
|
1.0
|
O2G
|
F:0KX401
|
4.7
|
66.6
|
1.0
|
C
|
F:ASP10
|
4.7
|
72.6
|
1.0
|
O1A
|
F:0KX401
|
4.7
|
60.1
|
1.0
|
O
|
F:ASP8
|
4.8
|
78.2
|
1.0
|
N
|
F:PHE12
|
4.8
|
76.9
|
1.0
|
CB
|
F:PHE12
|
4.8
|
77.8
|
1.0
|
O
|
F:ASP103
|
5.0
|
73.8
|
1.0
|
|
Magnesium binding site 2 out
of 4 in 6jup
Go back to
Magnesium Binding Sites List in 6jup
Magnesium binding site 2 out
of 4 in the Mutant Poliv-Dna Incoming Nucleotide Complex
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Mutant Poliv-Dna Incoming Nucleotide Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Mg403
b:56.8
occ:1.00
|
OE1
|
F:GLU104
|
1.9
|
98.2
|
1.0
|
OD2
|
F:ASP103
|
2.4
|
81.4
|
1.0
|
O2A
|
F:0KX401
|
2.5
|
85.1
|
1.0
|
OD1
|
F:ASP103
|
2.6
|
0.2
|
1.0
|
OD2
|
F:ASP8
|
2.6
|
89.2
|
1.0
|
OD1
|
F:ASP8
|
2.7
|
0.8
|
1.0
|
CG
|
F:ASP103
|
2.8
|
65.2
|
1.0
|
CG
|
F:ASP8
|
2.9
|
81.2
|
1.0
|
CD
|
F:GLU104
|
3.1
|
81.4
|
1.0
|
O3'
|
H:DC873
|
3.3
|
83.7
|
1.0
|
MG
|
F:MG402
|
3.3
|
58.6
|
1.0
|
C3'
|
H:DC873
|
3.4
|
73.7
|
1.0
|
PA
|
F:0KX401
|
3.6
|
69.3
|
1.0
|
O5'
|
F:0KX401
|
3.7
|
61.2
|
1.0
|
CB
|
F:GLU104
|
3.8
|
71.8
|
1.0
|
CG
|
F:GLU104
|
3.9
|
70.5
|
1.0
|
OE2
|
F:GLU104
|
4.0
|
82.4
|
1.0
|
O1A
|
F:0KX401
|
4.2
|
60.1
|
1.0
|
C5'
|
F:0KX401
|
4.2
|
64.7
|
1.0
|
OG
|
F:SER101
|
4.3
|
80.2
|
1.0
|
CB
|
F:ASP103
|
4.3
|
69.0
|
1.0
|
CB
|
F:ASP8
|
4.3
|
81.8
|
1.0
|
C4'
|
H:DC873
|
4.3
|
81.7
|
1.0
|
O
|
F:ASP103
|
4.3
|
73.8
|
1.0
|
O2B
|
F:0KX401
|
4.4
|
97.2
|
1.0
|
C2'
|
H:DC873
|
4.5
|
92.7
|
1.0
|
C
|
F:ASP103
|
4.5
|
81.4
|
1.0
|
C5'
|
H:DC873
|
4.6
|
75.8
|
1.0
|
CA
|
F:GLU104
|
4.7
|
76.5
|
1.0
|
O1G
|
F:0KX401
|
4.7
|
0.5
|
1.0
|
CA
|
F:ASP8
|
4.8
|
83.7
|
1.0
|
N
|
F:GLU104
|
4.9
|
77.2
|
1.0
|
O
|
F:MET9
|
5.0
|
72.4
|
1.0
|
O5'
|
H:DC873
|
5.0
|
84.4
|
1.0
|
|
Magnesium binding site 3 out
of 4 in 6jup
Go back to
Magnesium Binding Sites List in 6jup
Magnesium binding site 3 out
of 4 in the Mutant Poliv-Dna Incoming Nucleotide Complex
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Mutant Poliv-Dna Incoming Nucleotide Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg402
b:85.0
occ:1.00
|
O2B
|
A:0KX401
|
1.8
|
94.8
|
1.0
|
OD1
|
A:ASP8
|
1.9
|
1.0
|
1.0
|
OD2
|
A:ASP103
|
2.0
|
0.7
|
1.0
|
O
|
A:MET9
|
2.1
|
93.9
|
1.0
|
O1A
|
A:0KX401
|
2.1
|
97.9
|
1.0
|
O1G
|
A:0KX401
|
2.2
|
0.1
|
1.0
|
CG
|
A:ASP8
|
3.0
|
0.9
|
1.0
|
MG
|
A:MG403
|
3.1
|
0.4
|
1.0
|
PB
|
A:0KX401
|
3.1
|
91.5
|
1.0
|
C
|
A:MET9
|
3.2
|
97.1
|
1.0
|
CG
|
A:ASP103
|
3.3
|
0.1
|
1.0
|
PA
|
A:0KX401
|
3.3
|
92.6
|
1.0
|
OD2
|
A:ASP8
|
3.4
|
0.4
|
1.0
|
PG
|
A:0KX401
|
3.5
|
91.8
|
1.0
|
N
|
A:MET9
|
3.7
|
1.0
|
1.0
|
N3A
|
A:0KX401
|
3.7
|
93.4
|
1.0
|
O3B
|
A:0KX401
|
3.8
|
95.8
|
1.0
|
C5'
|
A:0KX401
|
3.8
|
93.5
|
1.0
|
OD1
|
A:ASP103
|
3.9
|
98.2
|
1.0
|
O5'
|
A:0KX401
|
4.0
|
92.9
|
1.0
|
CA
|
A:MET9
|
4.0
|
0.1
|
1.0
|
O2G
|
A:0KX401
|
4.1
|
0.7
|
1.0
|
CB
|
A:ASP8
|
4.2
|
0.1
|
1.0
|
O1B
|
A:0KX401
|
4.3
|
93.9
|
1.0
|
N
|
A:ASP10
|
4.3
|
0.2
|
1.0
|
C
|
A:ASP8
|
4.3
|
99.1
|
1.0
|
CB
|
A:ASP103
|
4.3
|
0.2
|
1.0
|
N
|
A:CYS11
|
4.4
|
0.3
|
1.0
|
CA
|
A:ASP10
|
4.5
|
0.1
|
1.0
|
O2A
|
A:0KX401
|
4.5
|
89.5
|
1.0
|
N
|
A:PHE12
|
4.6
|
0.5
|
1.0
|
CB
|
A:MET9
|
4.6
|
0.5
|
1.0
|
CA
|
A:ASP8
|
4.6
|
0.3
|
1.0
|
O3G
|
A:0KX401
|
4.7
|
97.4
|
1.0
|
CB
|
A:PHE12
|
4.7
|
0.1
|
1.0
|
C
|
A:ASP10
|
4.8
|
0.3
|
1.0
|
NZ
|
A:LYS157
|
4.8
|
0.1
|
1.0
|
OE1
|
A:GLU104
|
4.9
|
0.7
|
1.0
|
O
|
A:ASP103
|
4.9
|
0.4
|
1.0
|
|
Magnesium binding site 4 out
of 4 in 6jup
Go back to
Magnesium Binding Sites List in 6jup
Magnesium binding site 4 out
of 4 in the Mutant Poliv-Dna Incoming Nucleotide Complex
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Mutant Poliv-Dna Incoming Nucleotide Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg403
b:0.4
occ:1.00
|
OE1
|
A:GLU104
|
2.1
|
0.7
|
1.0
|
OD2
|
A:ASP8
|
2.3
|
0.4
|
1.0
|
OD1
|
A:ASP103
|
2.3
|
98.2
|
1.0
|
O1A
|
A:0KX401
|
2.3
|
97.9
|
1.0
|
CG
|
A:GLU104
|
2.5
|
0.4
|
1.0
|
OD1
|
A:ASP8
|
2.5
|
1.0
|
1.0
|
CG
|
A:ASP8
|
2.6
|
0.9
|
1.0
|
OD2
|
A:ASP103
|
2.6
|
0.7
|
1.0
|
CD
|
A:GLU104
|
2.6
|
0.4
|
1.0
|
CG
|
A:ASP103
|
2.8
|
0.1
|
1.0
|
MG
|
A:MG402
|
3.1
|
85.0
|
1.0
|
O3'
|
C:DC873
|
3.2
|
98.2
|
1.0
|
PA
|
A:0KX401
|
3.4
|
92.6
|
1.0
|
C3'
|
C:DC873
|
3.6
|
97.7
|
1.0
|
O5'
|
A:0KX401
|
3.7
|
92.9
|
1.0
|
OE2
|
A:GLU104
|
3.9
|
1.0
|
1.0
|
O2A
|
A:0KX401
|
3.9
|
89.5
|
1.0
|
CB
|
A:GLU104
|
3.9
|
98.5
|
1.0
|
CB
|
A:ASP8
|
3.9
|
0.1
|
1.0
|
O
|
A:ASP103
|
4.1
|
0.4
|
1.0
|
C5'
|
A:0KX401
|
4.2
|
93.5
|
1.0
|
C
|
A:ASP103
|
4.2
|
0.9
|
1.0
|
CB
|
A:ASP103
|
4.2
|
0.2
|
1.0
|
O2B
|
A:0KX401
|
4.4
|
94.8
|
1.0
|
O1G
|
A:0KX401
|
4.4
|
0.1
|
1.0
|
CA
|
A:ASP8
|
4.4
|
0.3
|
1.0
|
N
|
A:GLU104
|
4.5
|
0.3
|
1.0
|
CA
|
A:GLU104
|
4.6
|
1.0
|
1.0
|
C2'
|
C:DC873
|
4.6
|
98.4
|
1.0
|
N
|
A:MET9
|
4.8
|
1.0
|
1.0
|
C4'
|
C:DC873
|
4.8
|
99.5
|
1.0
|
O
|
A:MET9
|
4.8
|
93.9
|
1.0
|
CA
|
A:ASP103
|
4.8
|
0.9
|
1.0
|
OG
|
A:SER101
|
4.9
|
99.2
|
1.0
|
N3A
|
A:0KX401
|
4.9
|
93.4
|
1.0
|
|
Reference:
M.K.Johnson,
J.Kottur,
D.T.Nair.
A Polar Filter in Dna Polymerases Prevents Ribonucleotide Incorporation. Nucleic Acids Res. V. 47 10693 2019.
ISSN: ESSN 1362-4962
PubMed: 31544946
DOI: 10.1093/NAR/GKZ792
Page generated: Tue Oct 1 05:48:00 2024
|