Magnesium in PDB 6m0e: Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose

All present enzymatic activity of Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose:
2.4.1.10;

The structure of Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose, PDB code: 6m0e was solved by T.Tonozuka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.20 / 1.35
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.377, 81.521, 99.081, 90.00, 90.00, 90.00
R / Rfree (%)	15 / 16.6

The binding sites of Magnesium atom in the Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose (pdb code 6m0e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose, PDB code: 6m0e:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:18.6 occ:1.00 O A:HOH804 2.1 16.6 1.0 O A:HOH867 2.1 20.4 1.0 O A:HOH1219 2.2 28.4 1.0 O A:HOH940 2.3 23.6 1.0 O A:HOH1170 4.0 29.4 1.0 O A:HOH817 4.1 37.0 1.0 O A:ARG396 4.2 10.9 1.0 OD1 A:ASP404 4.3 15.0 1.0 O A:HOH1069 4.4 22.6 1.0 O A:VAL403 4.4 15.8 1.0 O A:TYR399 4.5 12.5 1.0 CB A:TYR399 4.7 10.3 1.0 CG A:ASP404 4.7 16.2 1.0

Magnesium binding site 2 out of 3 in the Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:22.7 occ:1.00 ND2 A:ASN451 2.2 19.4 1.0 O A:HOH1095 2.4 45.9 1.0 O A:HOH1153 2.4 39.6 1.0 O A:HOH1075 2.5 26.6 1.0 CG A:ASN451 3.5 17.8 1.0 CA A:ASN451 4.1 16.1 1.0 CB A:ASN451 4.1 16.8 1.0 OD1 A:ASN451 4.4 20.3 1.0 O A:PRO450 4.9 18.0 1.0 N A:ASN451 5.0 15.2 1.0

Magnesium binding site 3 out of 3 in the Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Beijerinckia Indica Beta-Fructosyltransferase Complexed with Fructose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:32.9 occ:1.00 OD2 A:ASP171 2.3 22.4 1.0 O A:HOH945 2.8 18.3 1.0 O A:HOH1096 3.1 28.7 1.0 CG A:ASP171 3.4 18.8 1.0 OD1 A:ASP171 3.7 18.4 1.0 O A:HOH1008 4.1 20.0 1.0 CB A:ASP171 4.6 16.5 1.0

T.Tonozuka, J.Kitamura, M.Nagaya, R.Kawai, A.Nishikawa, K.Hirano, K.Tamura, T.Fujii, T.Tochio. Crystal Structure of A Glycoside Hydrolase Family 68 Beta-Fructosyltransferase From Beijerinckia Indica Subsp. Indica in Complex with Fructose. Biosci.Biotechnol.Biochem. V. 84 2508 2020.
ISSN: ISSN 0916-8451
PubMed: 32752982
DOI: 10.1080/09168451.2020.1804317