Magnesium in PDB 6m0v: Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam

The structure of Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam, PDB code: 6m0v was solved by Y.Zhang, H.Zhang, X.Xu, Y.Wang, W.Chen, Y.Wang, Z.Wu, N.Tang, Y.Wang, S.Zhao, J.Gan, Q.Ji, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.82 / 3.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	321.839, 75.078, 70.109, 90.00, 93.21, 90.00
R / Rfree (%)	21.3 / 25.7

The structure of Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam also contains other interesting chemical elements:

Barium

(Ba)

10 atoms

The binding sites of Magnesium atom in the Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam (pdb code 6m0v). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam, PDB code: 6m0v:

Magnesium binding site 1 out of 1 in the Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crsytal Structure of Streptococcus Thermophilus CAS9 in Complex with the Ggaa Pam within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1206 b:41.0 occ:1.00 OD2 A:ASP598 2.2 62.3 1.0 OD1 A:ASN622 2.3 41.5 1.0 OP1 C:DC12 2.4 52.0 1.0 O3' C:DC11 2.6 41.7 1.0 P C:DC12 3.0 36.0 1.0 CG A:ASP598 3.2 52.0 1.0 CG A:ASN622 3.5 42.0 1.0 CB A:ASP598 3.7 43.7 1.0 C3' C:DC11 3.8 35.5 1.0 O A:ALA599 3.9 43.6 1.0 O5' C:DC12 3.9 37.1 1.0 O A:ASN622 4.0 47.5 1.0 ND2 A:ASN622 4.0 49.2 1.0 CA A:ASP598 4.1 43.3 1.0 C4' C:DC11 4.1 34.7 1.0 N A:ALA599 4.1 44.3 1.0 O A:GLY626 4.1 50.1 1.0 NZ A:LYS625 4.2 44.2 1.0 OP2 C:DC12 4.3 35.8 1.0 OD1 A:ASP598 4.3 54.7 1.0 C A:ASP598 4.5 44.3 1.0 C5' C:DC11 4.6 36.3 1.0 CD A:LYS625 4.6 43.9 1.0 C2' C:DC11 4.6 37.9 1.0 CB A:ASN622 4.7 44.2 1.0 C A:ASN622 4.7 44.8 1.0 C A:ALA599 4.8 47.0 1.0 CA A:ASN622 4.8 45.6 1.0 CG A:LYS625 4.9 43.1 1.0 CB A:LYS625 4.9 44.6 1.0 CA A:ALA599 4.9 45.5 1.0

Y.Zhang, H.Zhang, X.Xu, Y.Wang, W.Chen, Y.Wang, Z.Wu, N.Tang, Y.Wang, S.Zhao, J.Gan, Q.Ji. Catalytic-State Structure and Engineering of Streptococcus Thermophilus CAS9 Nat Catal 2020.
ISSN: ESSN 2520-1158
DOI: 10.1038/S41929-020-00506-9