Magnesium in PDB 6n53: Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate

All present enzymatic activity of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate:
2.7.1.48;

The structure of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate, PDB code: 6n53 was solved by B.J.Cuthbert, S.Nainar, R.C.Spitale, C.W.Goulding, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.82 / 2.70
Space group	F 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	89.896, 142.404, 248.566, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 24.4

The binding sites of Magnesium atom in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate (pdb code 6n53). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate, PDB code: 6n53:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:99.6 occ:1.00 OE1 A:GLU135 3.1 89.2 1.0 OD2 A:ASP62 3.4 0.5 1.0 OP1 A:UZR304 3.5 0.1 0.8 OE2 A:GLU135 3.6 80.3 1.0 CD A:GLU135 3.7 86.5 1.0 O4 A:PO4301 3.8 55.0 1.0 OP3 A:UZR304 3.9 79.0 0.8 P A:UZR304 4.3 94.0 0.8 O3 A:PO4301 4.3 59.8 1.0 CG A:ASP62 4.5 0.8 1.0 NH1 A:ARG169 4.7 82.6 1.0 P A:PO4301 4.7 64.9 1.0 CB A:SER34 4.8 50.3 1.0 OP2 A:UZR304 4.9 0.8 0.8 OG A:SER34 5.0 63.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidouridine Monophosphate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:82.5 occ:1.00 O3 B:PO4301 2.3 52.2 1.0 O B:HOH423 2.8 76.6 1.0 OE1 B:GLU135 3.1 81.3 1.0 OE2 B:GLU135 3.3 70.5 1.0 CD B:GLU135 3.5 87.9 1.0 P B:PO4301 3.5 64.0 1.0 O4 B:PO4301 3.5 60.2 1.0 OG B:SER34 3.6 91.4 1.0 CB B:SER34 3.7 53.9 1.0 O B:HOH404 4.0 69.8 1.0 N B:SER34 4.2 58.0 1.0 O2 B:PO4301 4.3 56.2 1.0 OP3 B:UZR307 4.3 73.6 0.7 CA B:SER34 4.5 57.9 1.0 O1 B:PO4301 4.6 50.9 1.0 NZ B:LYS33 4.7 56.6 1.0 NH1 B:ARG169 4.7 70.4 1.0 CG B:GLU135 4.7 54.8 1.0 O B:HOH401 4.8 59.2 1.0 CE B:LYS33 4.8 53.9 1.0 CB B:LYS33 4.8 61.3 1.0 OP1 B:UZR307 4.9 92.6 0.7

S.Nainar, B.J.Cuthbert, C.W.Goulding, R.C.Spitale. Incorporation of Novel Azido-Nucleotides Into Rna To Be Published.