Magnesium in PDB 6n54: Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate

All present enzymatic activity of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate:
2.7.1.48;

The structure of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate, PDB code: 6n54 was solved by B.J.Cuthbert, S.Nainar, R.C.Spitale, C.W.Goulding, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.58 / 2.42
Space group	F 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	90.329, 141.263, 247.887, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 22.7

The binding sites of Magnesium atom in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate (pdb code 6n54). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate, PDB code: 6n54:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:86.5 occ:1.00 O A:HOH403 2.1 87.0 1.0 O A:HOH450 2.4 75.2 1.0 O3 A:PO4303 2.8 50.9 1.0 OE2 A:GLU135 3.2 78.2 1.0 OE1 A:GLU135 3.5 67.0 1.0 O A:HOH411 3.6 72.2 1.0 O1 A:PO4303 3.7 53.0 1.0 O08 A:KEA304 3.7 43.8 0.9 CD A:GLU135 3.7 66.3 1.0 P A:PO4303 3.8 51.0 1.0 OG A:SER34 4.2 59.8 1.0 O09 A:KEA304 4.2 82.9 0.9 CB A:SER34 4.3 42.8 1.0 OD1 A:ASP62 4.4 87.9 1.0 P06 A:KEA304 4.5 74.8 0.9 OG A:SER60 4.7 90.1 1.0 O05 A:KEA304 4.7 96.0 0.9 CE A:LYS33 4.8 35.4 1.0 O2 A:PO4303 4.8 48.2 1.0 NZ A:LYS33 4.8 40.3 1.0 NH1 A:ARG169 4.8 66.5 1.0 O4 A:PO4303 4.8 43.7 1.0 N A:SER34 4.9 46.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Uridine-Cytidine Kinase 2 Complexed with 2'-Azidocytidine Monophosphate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg301 b:73.3 occ:1.00 O B:HOH426 2.3 81.7 1.0 O1 B:PO4308 2.6 45.0 1.0 O B:HOH451 2.6 64.3 1.0 OE2 B:GLU135 3.0 75.7 1.0 OE1 B:GLU135 3.0 65.2 1.0 O B:HOH446 3.1 59.6 1.0 CD B:GLU135 3.4 59.5 1.0 O4 B:PO4308 3.7 54.4 1.0 P B:PO4308 3.8 49.4 1.0 O07 B:KEA309 3.8 46.4 0.7 CB B:SER34 4.2 53.8 1.0 OG B:SER34 4.3 83.6 1.0 O08 B:KEA309 4.4 0.0 0.7 N B:SER34 4.6 46.1 1.0 O2 B:PO4308 4.7 41.5 1.0 CE B:LYS33 4.7 34.0 1.0 P06 B:KEA309 4.7 87.5 0.7 OD2 B:ASP62 4.7 94.0 1.0 CG B:GLU135 4.7 52.8 1.0 NZ B:LYS33 4.8 36.4 1.0 O3 B:PO4308 4.8 44.1 1.0 OG B:SER60 4.9 71.6 1.0 CA B:SER34 5.0 56.8 1.0 NH1 B:ARG169 5.0 57.6 1.0

S.Nainar, B.J.Cuthbert, C.W.Goulding, R.C.Spitale. Incorporation of Novel Azido-Nucleotides Into Rna To Be Published.