Atomistry » Magnesium » PDB 6mxd-6n6n » 6n60
Atomistry »
  Magnesium »
    PDB 6mxd-6n6n »
      6n60 »

Magnesium in PDB 6n60: Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)

Enzymatic activity of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)

All present enzymatic activity of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25):
2.7.7.6;

Protein crystallography data

The structure of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25), PDB code: 6n60 was solved by N.Braffman, J.Hauver, E.A.Campbell, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.55 / 3.68
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 172.906, 172.906, 387.263, 90.00, 90.00, 90.00
R / Rfree (%) 26.3 / 30.6

Other elements in 6n60:

The structure of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) (pdb code 6n60). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25), PDB code: 6n60:

Magnesium binding site 1 out of 1 in 6n60

Go back to Magnesium Binding Sites List in 6n60
Magnesium binding site 1 out of 1 in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg1501

b:83.6
occ:1.00
OD2 D:ASP462 2.4 0.3 1.0
OD1 D:ASP462 2.5 0.2 1.0
OD2 D:ASP464 2.6 0.4 1.0
OD1 D:ASP464 2.7 0.4 1.0
OD2 D:ASP460 2.8 0.0 1.0
CG D:ASP462 2.8 0.9 1.0
CG D:ASP464 3.0 0.9 1.0
CB D:ASP460 3.2 0.6 1.0
CG D:ASP460 3.4 0.3 1.0
N D:ASP460 4.1 0.6 1.0
CA D:ASP460 4.1 0.6 1.0
NH1 D:ARG425 4.2 0.4 1.0
CB D:ASP462 4.3 0.5 1.0
NH2 D:ARG425 4.4 0.9 1.0
CB D:ASP464 4.5 0.4 1.0
OD1 D:ASP460 4.6 0.5 1.0
C D:ASP460 4.6 0.8 1.0
O D:ASP460 4.8 0.7 1.0
CZ D:ARG425 4.8 0.1 1.0
N D:ASP462 4.9 0.3 1.0

Reference:

N.R.Braffman, F.J.Piscotta, J.Hauver, E.A.Campbell, A.J.Link, S.A.Darst. Structural Mechanism of Transcription Inhibition By Lasso Peptides Microcin J25 and Capistruin. Proc. Natl. Acad. Sci. V. 116 1273 2019U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30626643
DOI: 10.1073/PNAS.1817352116
Page generated: Tue Oct 1 12:30:04 2024

Last articles

Mn in 1V00
Mn in 1UZZ
Mn in 1V1M
Mn in 1V1F
Mn in 1V16
Mn in 1UVK
Mn in 1UVL
Mn in 1V11
Mn in 1UVM
Mn in 1UVJ
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy