Magnesium in PDB 6n60: Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)

Enzymatic activity of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)

All present enzymatic activity of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25):
2.7.7.6;

Protein crystallography data

The structure of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25), PDB code: 6n60 was solved by N.Braffman, J.Hauver, E.A.Campbell, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	49.55 / 3.68
*Space group*	P 4₁ 2₁ 2
*Cell size a, b, c (Å), α, β, γ (°)*	172.906, 172.906, 387.263, 90.00, 90.00, 90.00
*R / R_free (%)*	26.3 / 30.6

Other elements in 6n60:

The structure of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) (pdb code 6n60). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25), PDB code: 6n60:

Magnesium binding site 1 out of 1 in 6n60

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Magnesium Binding Sites List in 6n60

Magnesium binding site 1 out of 1 in the Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25)

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Rna Polymerase SIGMA70-Holoenzyme Bound to Upstream Fork Promoter Dna and Microcin J25 (MCCJ25) within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
D:Mg1501 b:83.6 occ:1.00	OD2	D:ASP462	2.4	0.3	1.0
	OD1	D:ASP462	2.5	0.2	1.0
	OD2	D:ASP464	2.6	0.4	1.0
	OD1	D:ASP464	2.7	0.4	1.0
	OD2	D:ASP460	2.8	0.0	1.0
	CG	D:ASP462	2.8	0.9	1.0
	CG	D:ASP464	3.0	0.9	1.0
	CB	D:ASP460	3.2	0.6	1.0
	CG	D:ASP460	3.4	0.3	1.0
	N	D:ASP460	4.1	0.6	1.0
	CA	D:ASP460	4.1	0.6	1.0
	NH1	D:ARG425	4.2	0.4	1.0
	CB	D:ASP462	4.3	0.5	1.0
	NH2	D:ARG425	4.4	0.9	1.0
	CB	D:ASP464	4.5	0.4	1.0
	OD1	D:ASP460	4.6	0.5	1.0
	C	D:ASP460	4.6	0.8	1.0
	O	D:ASP460	4.8	0.7	1.0
	CZ	D:ARG425	4.8	0.1	1.0
	N	D:ASP462	4.9	0.3	1.0

Reference:

N.R.Braffman, F.J.Piscotta, J.Hauver, E.A.Campbell, A.J.Link, S.A.Darst. Structural Mechanism of Transcription Inhibition By Lasso Peptides Microcin J25 and Capistruin. Proc. Natl. Acad. Sci. V. 116 1273 2019U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30626643
DOI: 10.1073/PNAS.1817352116

Page generated: Tue Oct 1 12:30:04 2024

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