Magnesium in PDB 6n98: Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1

All present enzymatic activity of Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1:
5.3.1.5;

The structure of Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1, PDB code: 6n98 was solved by R.Y.Miyamoto, P.S.Vieira, M.T.Murakami, L.M.Zanphorlin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.77 / 1.55
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	87.100, 92.900, 99.300, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 21.4

The binding sites of Magnesium atom in the Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1 (pdb code 6n98). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1, PDB code: 6n98:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg407 b:27.5 occ:1.00 OE1 A:GLU215 1.9 36.6 1.0 OD2 A:ASP285 2.1 35.3 1.0 OD2 A:ASP243 2.4 34.1 1.0 OE2 A:GLU179 2.6 33.4 1.0 CD A:GLU179 3.1 28.2 1.0 CD A:GLU215 3.1 27.5 1.0 CG A:ASP285 3.2 27.4 1.0 OE1 A:GLU179 3.2 25.8 1.0 O A:HOH528 3.3 40.1 1.0 O A:HOH635 3.3 39.7 1.0 CG A:ASP243 3.5 30.4 1.0 CB A:ASP285 3.7 20.5 1.0 OE2 A:GLU215 3.8 33.4 1.0 CB A:ASP243 4.0 22.9 1.0 O A:HOH578 4.0 44.0 1.0 O A:HOH699 4.1 27.2 1.0 OD1 A:ASP285 4.2 25.9 1.0 CG A:GLU215 4.3 19.9 1.0 CE1 A:HIS218 4.3 19.9 1.0 CG A:GLU179 4.3 22.7 1.0 CB A:GLU215 4.5 15.2 1.0 OD1 A:ASP243 4.5 35.1 1.0 ND2 A:ASN213 4.6 24.4 1.0 NE2 A:HIS218 4.6 18.8 1.0 O A:HOH710 4.9 31.1 1.0

Magnesium binding site 2 out of 2 in the Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg408 b:40.6 occ:1.00 OD2 A:ASP253 1.8 49.1 1.0 O A:HOH578 2.1 44.0 1.0 CG A:ASP253 2.5 41.7 1.0 OE2 A:GLU215 2.6 33.4 1.0 OD1 A:ASP253 2.7 42.8 1.0 OD1 A:ASP255 2.7 27.6 1.0 NE2 A:HIS218 2.9 18.8 1.0 O A:HOH710 3.3 31.1 1.0 CD2 A:HIS218 3.5 22.4 1.0 CG A:ASP255 3.5 26.6 1.0 OD2 A:ASP255 3.6 31.0 1.0 CD A:GLU215 3.7 27.5 1.0 CB A:ASP253 4.0 28.4 1.0 CE1 A:HIS218 4.1 19.9 1.0 ND2 A:ASN245 4.2 19.6 1.0 NZ A:LYS181 4.2 23.6 1.0 OE1 A:GLU215 4.3 36.6 1.0 CE A:LYS181 4.5 19.2 1.0 O A:HOH579 4.6 26.3 1.0 OD2 A:ASP285 4.6 35.3 1.0 CG A:HIS218 4.8 19.4 1.0 O A:HOH635 4.8 39.7 1.0 CG A:GLU215 4.8 19.9 1.0 CB A:ASP255 4.9 23.0 1.0

R.Y.Miyamoto, A.S.De Souza, P.S.Vieira, R.R.De Melo, J.A.Scarpassa, C.H.I.Ramos, M.T.Murakami, R.Ruller, L.M.Zanphorlin. Xylose Isomerase 1F1 Variant From Streptomyces Sp. F-1 To Be Published.