Magnesium in PDB 6njt: Mouse Endonuclease G Mutant - H97A

The structure of Mouse Endonuclease G Mutant - H97A, PDB code: 6njt was solved by C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.71 / 2.07
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	109.609, 109.609, 118.062, 90.00, 90.00, 120.00
R / Rfree (%)	18.4 / 21.3

The structure of Mouse Endonuclease G Mutant - H97A also contains other interesting chemical elements:

Chlorine

(Cl)

11 atoms

The binding sites of Magnesium atom in the Mouse Endonuclease G Mutant - H97A (pdb code 6njt). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Mouse Endonuclease G Mutant - H97A, PDB code: 6njt:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:20.6 occ:1.00 O A:HOH436 2.0 18.5 1.0 O A:HOH407 2.0 19.2 1.0 OD1 A:ASN128 2.1 21.4 1.0 O A:HOH524 2.1 16.0 1.0 O A:HOH522 2.2 21.9 1.0 O A:HOH429 2.2 18.8 1.0 CG A:ASN128 3.2 23.5 1.0 ND2 A:ASN128 3.9 18.0 1.0 OE2 A:GLU136 3.9 22.4 1.0 O A:HOH498 4.0 21.7 1.0 OE1 A:GLU136 4.0 26.4 1.0 N A:ALA97 4.1 22.0 1.0 O A:HOH563 4.2 33.2 1.0 O A:ASN128 4.2 20.4 1.0 OE1 A:GLN123 4.3 22.2 1.0 O A:HOH458 4.3 26.2 1.0 O A:ALA97 4.4 25.1 1.0 CD A:GLU136 4.4 28.7 1.0 O A:HOH556 4.4 29.3 1.0 O A:HOH601 4.4 22.3 1.0 CB A:ASN128 4.5 19.7 1.0 O A:HOH596 4.5 21.3 1.0 CD1 A:TRP132 4.6 19.9 1.0 CB A:ALA97 4.6 20.0 1.0 CA A:GLY96 4.7 20.3 1.0 CA A:ASN128 4.7 19.5 1.0 C A:ASN128 4.8 26.1 1.0 C A:GLY96 4.8 25.7 1.0 CA A:ALA97 4.8 18.3 1.0

Magnesium binding site 2 out of 2 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg301 b:21.7 occ:1.00 OD1 B:ASN128 2.0 17.9 1.0 O B:HOH422 2.0 21.9 1.0 O B:HOH437 2.1 17.4 1.0 O B:HOH561 2.1 22.6 1.0 O B:HOH435 2.2 23.7 1.0 O B:HOH550 2.3 22.7 1.0 CG B:ASN128 3.1 28.0 1.0 ND2 B:ASN128 3.8 18.1 1.0 OE2 B:GLU136 3.9 23.0 1.0 N B:ALA97 4.0 17.2 1.0 OE1 B:GLN123 4.1 25.8 1.0 OE1 B:GLU136 4.1 21.8 1.0 O B:HOH532 4.1 22.4 1.0 O B:ASN128 4.1 22.9 1.0 O B:HOH601 4.2 38.3 1.0 O B:HOH476 4.2 26.2 1.0 O B:ALA97 4.3 20.7 1.0 CB B:ASN128 4.4 19.1 1.0 CD B:GLU136 4.4 30.0 1.0 O B:HOH560 4.5 29.5 1.0 O B:HOH618 4.5 21.7 1.0 O B:HOH614 4.5 21.0 1.0 CA B:ASN128 4.6 25.5 1.0 CA B:GLY96 4.6 17.7 1.0 CD1 B:TRP132 4.7 20.6 1.0 C B:ASN128 4.7 26.6 1.0 CB B:ALA97 4.7 19.9 1.0 C B:GLY96 4.7 26.5 1.0 CA B:ALA97 4.8 23.7 1.0 C B:ALA97 5.0 25.8 1.0

C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho. Structural Adaptation of Vertebrate Endonuclease G For 5-Hydroxymethylcytosine Recognition and Function To Be Published.