Magnesium in PDB 6nw9: Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120

The structure of Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120, PDB code: 6nw9 was solved by J.Y.Greenfield, O.Herzberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.16 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	99.439, 66.770, 161.600, 90.00, 103.58, 90.00
R / Rfree (%)	15.1 / 18.4

The structure of Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120 also contains other interesting chemical elements:

Chlorine	(Cl)	6 atoms
Sodium	(Na)	3 atoms

The binding sites of Magnesium atom in the Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120 (pdb code 6nw9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120, PDB code: 6nw9:

Magnesium binding site 1 out of 1 in the Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of A Tailspike Protein 3 (TSP3, ORF212) From Escherichia Coli O157:H7 Bacteriophage CBA120 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg704 b:25.8 occ:0.62 O A:HOH846 2.1 34.7 1.0 O A:HOH844 2.1 34.6 1.0 O B:HOH967 2.1 37.4 1.0 OD1 A:ASN24 2.1 30.6 1.0 OD1 B:ASN24 2.2 29.1 1.0 OD1 C:ASN24 2.2 33.6 1.0 CG A:ASN24 3.0 33.2 1.0 CG C:ASN24 3.1 31.2 1.0 CG B:ASN24 3.1 32.5 1.0 ND2 A:ASN24 3.3 33.7 1.0 O B:HOH818 3.4 42.9 1.0 ND2 C:ASN24 3.4 34.7 1.0 ND2 B:ASN24 3.4 32.0 1.0 O A:SER20 3.8 27.3 1.0 O C:SER20 3.9 22.9 1.0 O B:SER20 4.0 32.6 1.0 OG A:SER20 4.2 37.6 1.0 CB A:ASN24 4.5 23.4 1.0 CB C:ASN24 4.5 20.0 1.0 CB B:ASN24 4.5 32.6 1.0 CB B:SER20 4.6 37.9 1.0 CB C:SER20 4.7 34.9 1.0 C C:SER20 4.7 25.9 1.0 C A:SER20 4.8 28.9 1.0 C B:SER20 4.8 32.0 1.0 N A:ASN24 4.8 27.1 1.0 CA A:ASN24 4.8 27.6 1.0 N B:ASN24 4.9 29.7 1.0 CA B:ASN24 4.9 36.5 1.0 CA C:ASN24 4.9 30.6 1.0 N C:ASN24 4.9 25.7 1.0

J.Greenfield, X.Shang, H.Luo, Y.Zhou, R.D.Heselpoth, D.C.Nelson, O.Herzberg. Structure and Tailspike Glycosidase Machinery of ORF212 From E. Coli O157:H7 Phage CBA120 (TSP3). Sci Rep V. 9 7349 2019.
ISSN: ESSN 2045-2322
PubMed: 31089181
DOI: 10.1038/S41598-019-43748-9